1. The overexpression of p16 is not a surrogate marker for high-risk human papilloma virus genotypes and predicts clinical outcomes for vulvar cancer
- Author
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Anton Żawrocki, Jacek J. Sznurkowski, and Wojciech Biernat
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,Vulvar Squamous Cell Carcinoma ,p16 ,Kaplan-Meier Estimate ,vSCC ,0302 clinical medicine ,Surgical oncology ,Stage (cooking) ,Papillomaviridae ,Aged, 80 and over ,Univariate analysis ,Vulvar cancer ,Vulvar Neoplasms ,Age Factors ,Middle Aged ,Prognosis ,Primary tumor ,Immunohistochemistry ,030220 oncology & carcinogenesis ,Lymphatic Metastasis ,Carcinoma, Squamous Cell ,Female ,Research Article ,Adult ,medicine.medical_specialty ,HPV ,Genotype ,03 medical and health sciences ,Internal medicine ,medicine ,Genetics ,Biomarkers, Tumor ,Cyclin-Dependent Kinase Inhibitor p18 ,Humans ,neoplasms ,Cyclin-Dependent Kinase Inhibitor p16 ,Aged ,Neoplasm Staging ,Retrospective Studies ,Surrogate endpoint ,Proportional hazards model ,business.industry ,Papillomavirus Infections ,medicine.disease ,030104 developmental biology ,DNA, Viral ,Radiotherapy, Adjuvant ,Neoplasm Grading ,business ,Follow-Up Studies - Abstract
Background We aimed to evaluate the correlation between p16ink4a-overexpression and high risk (hr)HPV-DNA in vulvar squamous cell carcinoma (vSCC) tumors as well as the impact of both biomarkers on the prognosis of vSCC patients. Methods PCR-detection of (hr)HPV-DNA and immunohistochemical staining for p16ink4a were conducted in 85 vSCC tumors. Survival analyses included the Kaplan–Meier method, log-rank test and Cox proportional hazards model. Results p16ink4a-overexpression and (hr)HPV-DNA were detected in 35 and 37 of the 85 tumors, respectively. Among the 35 p16ink4a-positive tumors, 10 lacked (hr)HPV-DNA (29 %). Among the 50 p16ink4a-negative tumors, (hr)HPV-DNA was detected in 12 cases (24 %). The median follow-up was 89.20 months (range 1.7–189.5 months). P16ink4a-overexpression, but not (hr)HPV-DNA positivity of the primary tumor, was correlated with prolonged overall survival (OS) (p = 0.009). P16ink4a-overexpression predicted a better response to radiotherapy (p
- Published
- 2016