1. Effects of protein concentration and detergent on endotoxin reduction by ultrafiltration
- Author
-
Seung-Cheol Moon, Hyo-Seung Kim, Hyun Zo Youn, Young-Rae Lee, Hyun M. Jang, Jong-Suk Kim, Kang-Yeoul Yu, Byeong-Kil Lee, Young Ju Jeong, Sung Ho Lee, and Byeong-Soo Kim
- Subjects
Lipopolysaccharides ,Gram-negative bacteria ,Low protein ,Lipopolysaccharide ,Detergents ,Ultrafiltration ,Polysaccharide ,Biochemistry ,Water Purification ,Lipid A ,Industrial Microbiology ,chemistry.chemical_compound ,Molecular Biology ,chemistry.chemical_classification ,biology ,Osmolar Concentration ,Proteins ,General Medicine ,biology.organism_classification ,Endotoxins ,Molecular Weight ,Monomer ,chemistry ,lipids (amino acids, peptides, and proteins) ,Bacterial outer membrane - Abstract
Lipopolysaccharide (LPS), found in the outer membrane of Gram negative bacteria, only exerts its toxic effects when in free form. LPS has three major parts, lipid A, the toxic compo-nent, along with a core polysaccharide and O-specific polysaccharide. LPS monomers are known to have molecular masses between 10 to 30 kDa. Under physiological con-ditions, LPS exists in equilibrium between monomer and vesi-cle forms. LPS removal by 100 kDa ultrafiltration was more ef-ficient (99.6% of LPS removed) with a low concentration of protein (2.0 mg/ml) compared to a high concentration (20.1 mg/ml). In the presence of different detergents (0.5% Tween 20, 1.0% taurodeoxycholate and 1.0% Triton X-100), LPS re-moval was more efficient at low protein concentrations (2.0 mg/ml) compared to high protein concentrations (20.1 mg/ml). [BMB reports 2009; 42(7): 462-466]
- Published
- 2009
- Full Text
- View/download PDF