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2 results on '"Dong-Hee Suk"'

1. Anti-oxidizing effect of the dichloromethane and hexane fractions from Orostachys japonicus in LPS-stimulated RAW 264.7 cells via upregulation of Nrf2 expression and activation of MAPK signaling pathway

Author

Hyeong-Seon Lee, Seon-Hee Kim, Dong-Hee Suk, Dong-Seok Lee, Hyun-Kyung Kim, and Gyeong-Seon Lee

Subjects
Lipopolysaccharides, MAPK/ERK pathway, Cell Survival, NF-E2-Related Factor 2, p38 mitogen-activated protein kinases, HO-1, Orostachys japonicus, Biology, Crassulaceae, medicine.disease_cause, p38 Mitogen-Activated Protein Kinases, Biochemistry, Antioxidants, Cell Line, Mice, Downregulation and upregulation, Anti-oxidizing effect, medicine, Animals, Hexanes, Nrf2/Keap1, Molecular Biology, Research Articles, RAW 264.7 Cells, Adaptor Proteins, Signal Transducing, Mitogen-Activated Protein Kinase Kinases, Methylene Chloride, Kelch-Like ECH-Associated Protein 1, Plant Extracts, General Medicine, MAPK, KEAP1, Up-Regulation, Cell biology, Cytoskeletal Proteins, Cell culture, Signal transduction, Heme Oxygenase-1, Oxidative stress, Signal Transduction
Abstract

Orostachys japonicus shows various biological activities. However, the molecular mechanisms remain unknown in LPS-stimulated macrophages. Here, we investigated the anti-oxidizing effect of the dichloromethane (DCM) and hexane fractions from O. japonicus (OJD and OJH) against oxidative stress in RAW 264.7 cells stimulated by LPS. OJD and OJH significantly increased the expression of heme oxygenase-1 (HO-1) in a dose- and time-dependent manner. Additionally, it was found that the expression of HO-1 was stimulated by Nrf2 activated via degradation of Keap1. ERK and p38 inhibitors repressed HO-1 induced by OJD and OJH in LPS-stimulated cells, respectively. In conclusion, these results suggest that OJD and OJH may block oxidative damage stimulated by LPS, via increasing the expression of HO-1 and Nrf2, and MAPK signaling pathway. [BMB Reports 2014; 47(2): 98-103]

Published
2014
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2. Anti-inflammatory effects of ethanol extract from Orostachys japonicus on modulation of signal pathways in LPS-stimulated RAW 264.7 cells

Author

Dong-Seok Lee, Jae Han Jeong, Deok Seon Ryu, and Dong Hee Suk

Subjects
Lipopolysaccharides, Anti-Inflammatory Agents, Transcription factor complex, Nitric Oxide Synthase Type II, Crassulaceae, Nitric Oxide, Biochemistry, Nitric oxide, Cell Line, chemistry.chemical_compound, Mice, Animals, Molecular Biology, Protein kinase B, PI3K/AKT/mTOR pathway, RAW 264.7 Cells, chemistry.chemical_classification, Inflammation, Reactive oxygen species, biology, Ethanol, Plant Extracts, Macrophages, General Medicine, Nitric oxide synthase, chemistry, biology.protein, Signal transduction, Reactive Oxygen Species, Signal Transduction
Abstract

In this study, powder of Orostachys japonicus A. Berger (O. japonicus) was extracted with 95% ethyl alcohol and fractionated using a series of organic solvents, including n-hexane (hexane), dichloromethane (DCM), ethylacetate (EtOAc), n-butanol (BuOH), and water (H2O). We investigated the anti-inflammatory effects of these O. japonicus extracts on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Their effects on the expression of inflammatory mediators and transcription factors were analyzed by Western blotting. DCM fraction significantly inhibited formation of reactive oxygen species (ROS) as well as nitric oxide (NO) in LPS-stimulated RAW 264.7 cells. Phosphorylation of the pro-inflammatory transcription factor complex nuclear factor-kappa B (NF-κB) p65 and expression of inducible nitric oxide synthase (iNOS), one of its downstream proteins, were also suppressed by DCM fraction. These effects were regulated by upsteam proteins in the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase/Akt (PI3K/Akt) signaling pathways. Taken together, our data suggest that O. japonicus could be used as a potential source for anti-inflammatory agents. [BMB reports 2011; 44(6): 399-404]

Published
2011

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