1. Suppression of TNF-alpha-induced MMP-9 expression by a cell-permeable superoxide dismutase in keratinocytes
- Author
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Soo Young Choi, Ha Yong Song, Ah Ra Goh, Sung Mi Ju, Jinseu Park, and Dong-Joo Kwon
- Subjects
Keratinocytes ,Cell Membrane Permeability ,Recombinant Fusion Proteins ,Inflammation ,medicine.disease_cause ,Biochemistry ,Cell Line ,Superoxide dismutase ,medicine ,Humans ,Molecular Biology ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Superoxide Dismutase ,Tumor Necrosis Factor-alpha ,NF-kappa B ,DNA ,General Medicine ,Molecular biology ,HaCaT ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,chemistry ,Cell culture ,biology.protein ,tat Gene Products, Human Immunodeficiency Virus ,Tumor necrosis factor alpha ,medicine.symptom ,Reactive Oxygen Species ,Keratinocyte ,Oxidative stress ,Protein Binding - Abstract
Up-regulation of selected matrix metalloproteinases (MMPs) such as MMP-9 contributes to inflammatory processes during the development of various skin diseases, such as atopic dermatitis. In this study, we examined the effect of a cell-permeable superoxide dismutase (Tat-SOD) on TNF-α-induced MMP-9 expression in human keratinocyte cells (HaCaT). When Tat-SOD was added to the culture medium of HaCaT cells, it rapidly entered the cells in dose- and time-dependent manners. Tat-SOD decreased TNF-α-induced reactive oxygen species (ROS) generation. Tat-SOD also inhibited TNF-α-induced NF-κB DNA binding activity. Treatment of HaCaT cells with Tat-SOD significantly inhibited TNF-α-induced mRNA and protein expression of MMP-9, as measured by RT-PCR and Western blot analysis. In addition, Tat-SOD suppressed TNF-α-induced gelatinolytic activity of MMP-9. Taken together, our results indicate that Tat-SOD can suppress TNF-α-induced MMP-9 expression via ROS-NF-κB-dependent mechanisms in keratinocytes, and therefore can be used as an immunomodulatory agent against inflammatory skin diseases related to oxidative stress. [BMB reports 2011; 44(7): 462-467]
- Published
- 2011