1. Methylation Status of Immune Response Genes Promoters in Cell-Free DNA Differs in Hemodialyzed Patients with Diabetic Nephropathy According to the Intensity of Anemia Therapy
- Author
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Magdalena Mokrejsova, Marie Korabecna, Eva Pazourkova, Ales Horinek, and Vladimir Tesar
- Subjects
Male ,Anemia ,Biology ,Diabetic nephropathy ,Immune system ,Renal Dialysis ,medicine ,Cluster Analysis ,Humans ,Diabetic Nephropathies ,Promoter Regions, Genetic ,Erythropoietin ,Gene Expression Profiling ,Immunity ,Promoter ,Hematology ,General Medicine ,Methylation ,DNA Methylation ,medicine.disease ,Cell-free fetal DNA ,Nephrology ,DNA methylation ,Immunology ,Cytokines ,Erythropoiesis ,Female - Abstract
Background: Anemia is a major complication of end-stage renal disease. Hemodialysis itself is regarded as a stimulus activating inflammation. Pro-inflammatory cytokines are able to suppress erythropoiesis. In this pilot study, we analyzed the changes in methylation status of promoters of immune response genes in cell-free DNA to detect the differences between diabetic subjects (n = 18) with different therapeutic doses of recombinant erythropoietin. Methods: The extent of promoter methylation of 24 genes in plasma cell-free DNA was examined before and after hemodialysis using EpiTect Methyl qPCR Array Inflammatory Response and Autoimmunity (Qiagen). Results: The patients with higher methylation status of gene sequences IL13RA1, IL15, EDG3 and INHA in interdialytic interval were significantly overrepresented in the group with none or mild anemia therapy. Conclusion: The results are in agreement with the fact that IL13 and IL15 are known inhibitors of erythropoiesis and with considered immunomodulatory role of cell-free DNA.
- Published
- 2013
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