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12. Activation of ULK1 Kinase Mediates Clearance of Free Alpha-Globin in Human Beta-Thalassemic Erythroblasts

Author

Lechauve, Christophe, primary, Keith, Julia, additional, Khandros, Eugene, additional, Fowler, Stephanie, additional, Mayberry, Kalin, additional, Freiwan, Abdullah, additional, Thom, Christopher, additional, Zhang, Jingjing, additional, Tillman, Heather, additional, Kundu, Mondira, additional, Motta, Irene, additional, Cappellini, Maria Domenica, additional, and Weiss, Mitchell J., additional

Published
2018
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13. Utility of Whole Exome Sequencing in Diagnosis of Pediatric Platelet Disorders: A Subanalysis of the Pediseq Study

Author

Romasko, Edward J, primary, Biswas, Sawona, additional, Devkota, Batsal, additional, Vijayakumar, Jayaraman, additional, Jairam, Sowmyra, additional, Thom, Christopher S, additional, Dulik, Matthew C, additional, Conlin, Laura K, additional, Spinner, Nancy B., additional, Krantz, Ian D, additional, and Lambert, Michele P., additional

Published
2016
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16. Manipulation of Tropomysin 1in iPSCs to Enhance in Vitro Blood Cell Production

Author

Thom, Christopher, Jobaliya, Chintan, Voight, Benjamin F, Chou, Stella P, and French, Deborah L

Abstract

Donor-derived blood transfusions are critical to our healthcare system, but do not fully meet the needs of patients with multiple alloantibodies, rare blood types, or HLA-sensitization. These needs have fueled the concept of ex vivoblood cell production, which might address issues related to demographic aging, infectious outbreaks transmitted by transfusions, and rare blood types. Blood cells produced in vitrocould be used for transfusions, and could also be used as blood bank testing reagents (Coleman et al, Transfusion2019). One major challenge in deploying this system is efficiently scaling up cell production.

Published
2020
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17. Tropomyosin 1Genetically Constrains in VitroMegakaryopoiesis

Author

Thom, Christopher, Jobaliya, Chintan, Lorenz, Kimberly, Maguire, Jean Ann, Gagne, Alyssa, Gadue, Paul, French, Deborah L, and Voight, Benjamin F

Abstract

Platelet size and count are heritable, quantifiable outcomes of hematopoiesis. A better understanding of genetic mechanisms that regulate hematopoiesis could yield insights into blood disorders and augment clinical translational efforts to generate platelets in vitro. For example, induced pluripotent stem cell-derived blood cells could someday ameliorate adverse effects and increase transfusable platelet supplies. However, these methods remain inefficient.

Published
2019
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18. The secreted lymphangiogenic factor CCBE1 is essential for fetal liver erythropoiesis.

Author

Zhiying Zou, Enis, David R., Hung Bui, Khandros, Eugene, Kumar, Vinayak, Jakus, Zoltan, Thom, Christopher, Yiqing Yang, Dhillon, Veerpal, Mei Chen, MinMin Lu, Weiss, Mitchell J., and Kahn, Mark L.

Subjects
*ERYTHROPOIESIS, *HEMATOPOIETIC system, *LYMPH circulation disorders, *STEM cell treatment, *CELL death
Abstract

The secreted protein CCBE1 is required for lymphatic vessel growth in fish and mice, and mutations in the CCBE1 gene cause Hennekam syndrome, a primary human lymphedema. Here we show that loss of CCBE1 also confers severe anemia in midgestation mouse embryos due to defective definitive erythropoiesis. Fetal liver erythroid precursors of Ccbe1 null mice exhibit reduced proliferation and increased apoptosis. Colony-forming assays and hematopoietic reconstitution studies suggest that CCBE1 promotes fetal liver erythropoiesis cell nonautonomously. Consistent with these findings, Ccbe1lacZ reporter expression is not detected in hematopoietic cells and conditional deletion of Ccbe1 in hematopoietic cells does not confer anemia. The expression of the erythropoietic factors erythropoietin and stem cell factor is preserved in CCBE1 null embryos, but erythroblastic island (EBI) formation is reduced due to abnormal macrophage function. In contrast to the profound effects on fetal liver erythropoiesis, postnatal deletion of Ccbe1 does not confer anemia, even under conditions of erythropoietic stress, and EBI formation is normal in the bone marrow of adult CCBE1 knockout mice. Our findings reveal that CCBE1 plays an essential role in regulating the fetal liver erythropoietic environment and suggest that EBI formation is regulated differently in the fetal liver and bone marrow. [ABSTRACT FROM AUTHOR]

Published
2013
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