1. Removal of HLA-A,B antigens from platelets
- Author
-
Paul Karl Horan, Debra Masel, Neil Blumberg, Joanna M. Heal, and Theodor K. Mayer
- Subjects
Blood Platelets ,Immunology ,Antibodies, Monoclonal ,Chloroquine ,Human leukocyte antigen ,Cell Biology ,Hematology ,Biology ,Biochemistry ,HLA-A ,Antigens present ,Antigen ,HLA Antigens ,HLA-B Antigens ,HLA-A2 Antigen ,medicine ,Tonicity ,Humans ,Platelet ,Lymphocytes ,medicine.drug - Abstract
Recent evidence suggests that HLA-A,B antigens present on human platelets, are acquired from plasma. Using hypertonic acid chloroquine (200 mg/ml, pH 5.0, 1 hr, 4 degrees C), we demonstrate elution of most or all HLA-A2 and HLA-B7,40 immunoreactive material from platelets. Corresponding antigens on human lymphocytes were not affected by this treatment. These findings are consistent with the hypothesis that HLA- A,B antigens are adsorbed onto platelet surfaces.
- Published
- 1984