320 results on '"Mediavilla, A"'
Search Results
2. Targeting Inflammatory Pathways to Reverse Immunosuppressive Tumor Microenvironment in Chronic Lymphocytic Leukemia
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Uriepero, Angimar, primary, Mediavilla-Varela, Melanie, additional, Maharaj, Kamira, additional, Gamal, Wael, additional, Marquez, Maria Elena, additional, Powers, John J., additional, Kunta, Vishaal, additional, Sahakian, Eva, additional, Oppezzo, Pablo, additional, and Pinilla Ibarz, Javier, additional
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- 2022
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3. Metabolic Abnormalities Associated with T-Cell Exhaustion in CLL Eµ-TCL1 Murine Model
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Gamal, Wael, primary, Mediavilla-Varela, Melanie, additional, Maharaj, Kamira, additional, Uriepero, Angimar, additional, Kunta, Vishaal, additional, Sahakian, Eva, additional, and Pinilla Ibarz, Javier, additional
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- 2022
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4. Efficacy of Combinatorial Treatment Approaches in CTCL
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Mediavilla-Varela, Melanie, primary, Sahakian, Eva, additional, Harro, Carly, additional, Powers, John J., additional, Conejo-Garcia, Jose, additional, Sokol, Lubomir, additional, and Pinilla Ibarz, Javier, additional
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- 2022
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5. Pooled Fecal Allogenic Microbiotherapy for Refractory Gastrointestinal Acute Graft-Versus-Host Disease: Results from the Early Access Program in France
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Malard, Florent, primary, Loschi, Michael, additional, Cluzeau, Thomas, additional, Legrand, Faezeh, additional, Mear, Jean-Baptiste, additional, Lhomme, Faustine, additional, Guenounou, Sarah, additional, Huynh, Anne, additional, Borel, Cecile, additional, Desmier, Deborah, additional, Moya, Niels, additional, Charbonnier, Amandine, additional, Lebon, Delphine, additional, Labussière-Wallet, Hélène, additional, Orvain, Corentin, additional, Chantepie, Sylvain, additional, Bulabois, Claude-Eric, additional, Camus, Vincent, additional, Couturier, Marie-Anne, additional, Cornillon, Jérôme, additional, Chevallier, Patrice, additional, Mediavilla, Clémence, additional, Ceballos, Patrice, additional, Beauvais, David, additional, Bruelle, Marion, additional, Plantamura, Emilie, additional, and Mohty, Mohamad, additional
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- 2022
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6. Efficacy of Combinatorial Treatment Approaches in CTCL
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Melanie Mediavilla-Varela, Eva Sahakian, Carly Harro, John J. Powers, Jose Conejo-Garcia, Lubomir Sokol, and Javier Pinilla Ibarz
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Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
7. Targeting Inflammatory Pathways to Reverse Immunosuppressive Tumor Microenvironment in Chronic Lymphocytic Leukemia
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Angimar Uriepero, Melanie Mediavilla-Varela, Kamira Maharaj, Wael Gamal, Maria Elena Marquez, John J. Powers, Vishaal Kunta, Eva Sahakian, Pablo Oppezzo, and Javier Pinilla Ibarz
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Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
8. Metabolic Abnormalities Associated with T-Cell Exhaustion in CLL Eµ-TCL1 Murine Model
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Wael Gamal, Melanie Mediavilla-Varela, Kamira Maharaj, Angimar Uriepero, Vishaal Kunta, Eva Sahakian, and Javier Pinilla Ibarz
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Immunology ,Cell Biology ,Hematology ,Biochemistry - Published
- 2022
9. Superiority of bortezomib, thalidomide, and dexamethasone (VTD) as induction pretransplantation therapy in multiple myeloma: a randomized phase 3 PETHEMA/GEM study
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Rosiñol, Laura, Oriol, Albert, Teruel, Ana Isabel, Hernández, Dolores, López-Jiménez, Javier, de la Rubia, Javier, Granell, Miquel, Besalduch, Joan, Palomera, Luis, González, Yolanda, Etxebeste, Mª Asunción, Díaz-Mediavilla, Joaquín, Hernández, Miguel T., de Arriba, Felipe, Gutiérrez, Norma C., Martín-Ramos, Mª Luisa, Cibeira, Mª Teresa, Mateos, Mª Victoria, Martínez, Joaquín, Alegre, Adrián, Lahuerta, Juan José, San Miguel, Jesús, and Bladé, Joan
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- 2012
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10. Efficacy of Vecabrutinib Treatment in a Murine Model of Sclerodermatous Graft-Versus-Host-Disease
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Maharaj, Kamira, primary, Mediavilla-Varela, Melanie, additional, Uriepero, Angimar, additional, Fox, Judith A., additional, Taverna, Pietro, additional, Gamal, Wael, additional, Sahakian, Eva, additional, and Pinilla Ibarz, Javier, additional
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- 2021
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11. Second Treatment Free Remission after Combination Therapy with Ruxolitinib Plus Tyrosine Kinase Inhibitors in Chronic Phase Chronic Myeloid Leukemia (CML)
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Sweet, Kendra, primary, Atallah, Ehab L., additional, Radich, Jerry P., additional, Zhang, Mei-Jie, additional, Sahakian, Eva, additional, Mediavilla-Varela, Melanie, additional, Vistocky, Alexis, additional, Heinrich, Michael C., additional, Thompson, James E., additional, Mauro, Michael J., additional, Flynn, Kathryn E, additional, Baim, Arielle, additional, and Pinilla Ibarz, Javier, additional
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- 2021
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12. Risk-adapted treatment of acute promyelocytic leukemia based on all-trans retinoic acid and anthracycline with addition of cytarabine in consolidation therapy for high-risk patients: further improvements in treatment outcome
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Sanz, Miguel A., Montesinos, Pau, Rayón, Chelo, Holowiecka, Alexandra, de la Serna, Javier, Milone, Gustavo, de Lisa, Elena, Brunet, Salut, Rubio, Vicente, Ribera, José M., Rivas, Concha, Krsnik, Isabel, Bergua, Juan, González, José, Díaz-Mediavilla, Joaquín, Rojas, Rafael, Manso, Félix, Ossenkoppele, Gert, González, José D., and Lowenberg, Bob
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- 2010
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13. Differentiation syndrome in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and anthracycline chemotherapy: characteristics, outcome, and prognostic factors
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Montesinos, Pau, Bergua, Juan M., Vellenga, Edo, Rayón, Chelo, Parody, Ricardo, de la Serna, Javier, León, Angel, Esteve, Jordi, Milone, Gustavo, Debén, Guillermo, Rivas, Concha, González, Marcos, Tormo, Mar, Díaz-Mediavilla, Joaquín, González, Jose D., Negri, Silvia, Amutio, Elena, Brunet, Salut, Lowenberg, Bob, and Sanz, Miguel A.
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- 2009
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14. Association between the proliferative rate of neoplastic B cells, their maturation stage, and underlying cytogenetic abnormalities in B-cell chronic lymphoproliferative disorders: analysis of a series of 432 patients
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Quijano, Sandra, López, Antonio, Rasillo, Ana, Barrena, Susana, Luz Sánchez, Maria, Flores, Juan, Fernández, Carlos, Sayagués, José María, Osuna, Carlos Salvador, Fernández, Nuria, González, Marcos, Giraldo, Pilar, Giralt, Manuel, Pérez, Maria Carmen, Martin-Antoran, José Manuel, Gutiérrez, Oliver, Perdiguer, Luis, Díaz Mediavilla, Joaquín, González Silva, Manuel, Asensio del Rio, Agustín, Cerveró, Carlos, Guerra, José Luis, Butrón, Rosario, del Carmen García, Maria, Almeida, Julia, and Orfao, Alberto
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- 2008
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15. Causes and prognostic factors of remission induction failure in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and idarubicin
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de la Serna, Javier, Montesinos, Pau, Vellenga, Edo, Rayón, Chelo, Parody, Ricardo, León, Angel, Esteve, Jordi, Bergua, Juan M., Milone, Gustavo, Debén, Guillermo, Rivas, Concha, González, Marcos, Tormo, Mar, Díaz-Mediavilla, Joaquín, González, Jose D., Negri, Silvia, Amutio, Elena, Brunet, Salut, Lowenberg, Bob, and Sanz, Miguel A.
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- 2008
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16. Second Treatment Free Remission after Combination Therapy with Ruxolitinib Plus Tyrosine Kinase Inhibitors in Chronic Phase Chronic Myeloid Leukemia (CML)
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Melanie Mediavilla-Varela, Alexis M Vistocky, Ehab Atallah, Michael J. Mauro, Jerry Radich, Arielle Baim, Kendra Sweet, Michael Heinrich, James E. Thompson, Kathryn E. Flynn, Javier Pinilla Ibarz, Eva Sahakian, and Mei-Jie Zhang
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Ruxolitinib ,Combination therapy ,business.industry ,Immunology ,Cancer research ,Medicine ,Cell Biology ,Hematology ,Chronic phase chronic myeloid leukemia ,business ,Biochemistry ,Tyrosine kinase ,medicine.drug - Abstract
Background: Discontinuation of tyrosine kinase inhibitors (TKIs) is feasible in a subset of CML patients who have maintained a deep molecular response for at least two years. Numerous discontinuation trials have been performed and consistently show approximately 50% of patients relapse after stopping TKIs. A recent study examining rates of treatment free remission (TFR) after a second attempt at stopping TKIs found, with a median follow up time of 38.3 months, 64.3% of patients had a molecular relapse (defined as a loss of major molecular response (MMR)). At 12, 24 and 36 months, TFR rates were 48%, 42% and 35%, respectively. These data suggest some patients with a history of molecular relapse upon TKI cessation could successfully stop treatment on a subsequent attempt, yet the majority will relapse a second time. 'Complete eradication' of CML remains elusive in most patients likely as a result of minimal residual disease (MRD), which is the result of BCR-ABL independent drug resistance. More specifically, CML cells that reside in sanctuary sites such as the bone marrow adhere to fibronectin and demonstrate cell adhesion mediated drug resistance (CAM-DR). The bone marrow microenvironment contains many cytokines and growth factors capable of inducing STAT3-Y705 phosphorylation via the JAK-STAT pathway leading to protection against TKI-induced cell death. Inhibiting JAK2 and TYK2 leads to complete inhibition of pSTAT3-Y705, thereby implicating the role of activation of JAK2 and TYK2 in STAT3-Y705 phosphorylation and resistance towards BCR-ABL TKI-induced cell death. A phase I clinical trial combined ruxolitinib, which inhibits JAK2 and TYK2, plus nilotinib in chronic phase (CP) CML patients and found that ruxolitinib 15mg PO BID was safe and well tolerated with 4/10 patients achieving undetectable BCR-ABL1 transcripts by PCR. Study Design and Methods: This single arm phase II study (NCT03610971) will enroll 41 subjects from the H Jean Khoury Cure CML Consortium. Eligible subjects must have a confirmed diagnosis of CP-CML and have previously attempted to discontinue TKI therapy per NCCN guidelines and had molecular recurrence, defined as loss of MMR, and were restarted on TKI. This trial combines ruxolitinib 15mg BID plus BCR-ABL TKI (imatinib, dasatinib, nilotinib or bosutinib) for 12 28-day cycles in the combination treatment phase (CTP). RQ-PCR to measure BCR-ABL transcripts will be checked at screening and every three months during the CTP. In the event that a subject experiences intolerance to a TKI, has confirmed loss of MMR, or loss of MR4.5 (>0.0032% IS) on two central PCR results, or discontinues ruxolitinib, the subject will be removed from CTP and enter into long term follow-up (LTFU). CTP phase will be followed by further RQ-PCR screening for the concurrent TFR phase. At this time ruxolitinib will be discontinued and any subject who has met the criteria for the TFR phase will be enrolled. During the TFR phase, subjects will discontinue their TKI and be monitored off treatment with RQ-PCR checked monthly for the first year, every six weeks for year two, and every 12 weeks during year three. Upon molecular recurrence, defined as loss of MMR, TKIs will be restarted. The primary endpoint is the 12-month TFR rate subsequent to completion of 12 cycles of combination therapy; however, subjects will remain in the TFR phase for three years. Therefore, the total duration of the trial will be approximately five years (one year on CTP + three years in the TFR phase + one-year LTFU). Study statistical design was calculated to yield a one-sided type I error rate of 0.025 and power of 65% when the true one-year relapse rate is 35%. This study will additionally assess patient-reported outcomes in conjunction with RQ-PCR testing. PROMIS and other measures will be self-administered through REDCap. Correlative studies will include comparing changes in pSTAT3 in K562 and KU812 cell lines using plasma from CML patients being treated with TKIs plus ruxolitinib, using the plasma inhibitory assay technique. Changes in pSTAT3 and pSTAT5 will be correlated with clinical response and rate of TFR. Additional correlatives include multiparameter flow-based assessment of the T-cell compartment (activity/polarization) as well as natural killer cell fractions in CML patients at various time points (TKIs alone, TKIs plus ruxolitinib and during TFR). Thus far, 14 patients have been enrolled. Disclosures Sweet: Astellas: Consultancy, Membership on an entity's Board of Directors or advisory committees; Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees; AROG: Membership on an entity's Board of Directors or advisory committees; Gilead: Membership on an entity's Board of Directors or advisory committees; Bristol Meyers Squibb: Honoraria, Membership on an entity's Board of Directors or advisory committees. Atallah: Amgen: Consultancy; BMS: Honoraria, Speakers Bureau; Novartis: Consultancy, Honoraria, Research Funding; Pfizer: Consultancy, Research Funding; Takeda: Consultancy, Research Funding; Abbvie: Consultancy, Speakers Bureau. Radich: Amgen: Membership on an entity's Board of Directors or advisory committees; Novartis: Membership on an entity's Board of Directors or advisory committees; Genentech: Membership on an entity's Board of Directors or advisory committees; BMS: Membership on an entity's Board of Directors or advisory committees. Thompson: Novartis/ Bristol-Myers Squibb: Research Funding. Mauro: Pfizer: Consultancy; Takeda: Consultancy; Bristol Myers Squibb: Consultancy, Research Funding; Novartis: Consultancy, Research Funding; Sun Pharma / SPARC: Research Funding. Pinilla Ibarz: AbbVie, Janssen, AstraZeneca, Novartis, TG Therapeutics, Takeda: Consultancy, Other: Advisory; Sellas: Other: ), patents/royalties/other intellectual property; MEI, Sunesis: Research Funding; AbbVie, Janssen, AstraZeneca, Takeda: Speakers Bureau. OffLabel Disclosure: Ruxolitinib is being used off-label in chronic myeloid leukemia
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- 2021
17. Efficacy of Vecabrutinib Treatment in a Murine Model of Sclerodermatous Graft-Versus-Host-Disease
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Pietro Taverna, Melanie Mediavilla-Varela, Wael Gamal, Eva Sahakian, Judith A. Fox, Angimar Uriepero, Javier Pinilla Ibarz, and Kamira Maharaj
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Graft-versus-host disease ,business.industry ,Murine model ,Immunology ,medicine ,Cell Biology ,Hematology ,medicine.disease ,business ,Biochemistry - Abstract
Introduction. Chronic graft-versus-host disease (cGVHD) can manifest as a complication in patients following allogeneic hematopoietic stem cell transplant resulting in morbidity and mortality. Effective treatment strategies for cGVHD are currently lacking. Ibrutinib, an irreversible BTK inhibitor with activity against several other Tec-family kinases, has been clinically developed for cGVHD treatment due to regulation of pathogenetic B cells and T-cell subsets. Vecabrutinib is a more selective, reversible inhibitor of BTK with a distinct kinase domain interaction, which could result in differentiated safety and activity profiles compared to ibrutinib. Vecabrutinib has the capacity to overcome the C481S mutation that mediates resistance to ibrutinib. Vecabrutinib also also demonstrates activity against ITK, which is expressed in T cells. In this study, we investigated the activity and immune modulation of vecabrutinib treatment in a murine model of sclerodermatous cGVHD. Ibrutinib was utilized for comparison. Methods. A murine model of sclerodermatous cGVHD was initiated by adoptive transfer of T-cell depleted bone marrow plus whole splenocytes from B10.D2 donors into BALB/c recipients that were previously subjected to sub-lethal irradiation. Total bone marrow and irradiation only groups were included as controls. Mice with established cGVHD characterized by weight loss and skin irritation symptoms were treated with vecabrutinib once daily at 50mg/kg by oral gavage or ibrutinib at 30mg/kg in drinking water 5 days per week for approximately 3 weeks beginning on day 27 post-adoptive transfer and ending on day 45 (n=10 mice per group). Body weight and clinical symptoms (appearance, activity, skin symptoms, diarrhea, conjunctivitis) were measured throughout the study. Immunophenotyping for B cells and T cells was performed on spleens collected from euthanized mice by flow cytometry at two timepoints (day 40 during treatment and on day 76 post-treatment). Levels of circulating immunoglobulins were measured by multiplex cytokine assay at both timepoints. Results. Clinical symptoms, including appearance, activity, skin irritation, redness, alopecia and diarrhea were significantly reduced in vecabrutinib- and ibrutinib-treated groups. Furthermore, there was a trend toward a more favorable clinical score overall for the vecabrutinib-treated group, however no statistical difference was observed compared to ibrutinib possibly due to small sample size. Weight loss was slightly elevated during vecabrutinib and ibrutinib treatment compared to vehicle (trend), however mice recovered body weight post-treatment and continued to maintain benefit. On day 40 (during treatment) and day 76 (post-treatment) groups of mice were euthanized for immunophenotyping analysis utilizing a 22-color flow cytometry panel. During treatment, both vecabrutinib and ibrutinib-treated mice retained total B cell numbers but exhibited reduced B-cell activation, proliferation, and number of B220+ CD138+ plasma cells. In addition, B cells secreted less IL-10, and IL-4/5. Expression of antigen-presentation molecules CD80 and CD86 on B cells were unchanged. Total CD3+ T cells, activated and proliferating CD4+ and CD8+ T cells, and cytotoxic granzyme-B+ CD8+ T cells were reduced in treated mice. Interestingly, CD4+ CD25+ FoxP3+ Treg number, expression of PD-1 on Tregs and CD4+ CXCR5+ PD-1+ T follicular helper cells were also reduced in both treatment groups. In addition, there were globally reduced numbers of cytokine-secreting CD4+ cells but no differences in Th1/Th2 or Th17/Treg ratios were observed. Post-treatment, proliferation and cytokine secretion of B cells and T cells were still lowered but less impaired than during treatment. Tregs and PD-1 expression were still reduced post-treatment. Finally, circulating levels of IgA were reduced during and post treatment in vecabrutinib-treated mice compared to vehicle, while IgG1, IgG2b were reduced in both treated groups. No changes in IgM levels were observed in either treatment group. In conclusion, vecabrutinib treatment demonstrated efficacy and beneficially regulated B cell and T cell immune subsets in a preclinical murine model of sclerodermatous cGVHD. Studies to further evaluate differences between vecabrutinib and ibrutinib treatment are ongoing. Figure 1 Figure 1. Disclosures Fox: Sunesis Pharmaceuticals: Current Employment. Taverna: Sunesis Pharmaceuticals: Current Employment. Pinilla Ibarz: Sellas: Other: ), patents/royalties/other intellectual property; MEI, Sunesis: Research Funding; AbbVie, Janssen, AstraZeneca, Takeda: Speakers Bureau; AbbVie, Janssen, AstraZeneca, Novartis, TG Therapeutics, Takeda: Consultancy, Other: Advisory.
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- 2021
18. TEAM Conditioning (Thiotepa, Etoposide, Cytarabine, Melphalan) Prior to Autologous Hematopoietic Stem Cell Transplantation for Hodgkin and Non-Hodgkin Lymphoma: Final Results from a Prospective Multicenter Study
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Duléry, Rémy, primary, Lebras, Laure, primary, Choquet, Sylvain, primary, Di Blasi, Roberta, primary, AL Jijakli, Ahmad Kanj, primary, Heuberger, Laurence, primary, Brissot, Eolia, primary, Battipaglia, Giorgia, primary, Malard, Florent, primary, Mediavilla, Clemence, primary, Vekhoff, Anne, primary, Coppo, Paul, primary, Ledraa, Tounes, primary, Marjanovic, Zora, primary, Corre, Elise, primary, Lapusan, Simona, primary, Aoudjhane, Malek, primary, Belhocine, Ramdane, primary, and Mohty, Mohamad, primary
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- 2019
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19. Investigating Antibiotic Exposure and Risk of Severe Acute Graft Versus Host Disease in Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation
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Mohty, Razan, primary, Dulery, Remy, additional, Battipaglia, Giorgia, additional, Brissot, Eolia, additional, Mediavilla, Clemence, additional, Sestili, Simona, additional, Ledraa, Tounes, additional, Gaugler, Béatrice, additional, Bonnin, Agnès, additional, Mohty, Mohamad, additional, and Malard, Florent, additional
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- 2019
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20. All-trans retinoic acid and anthracycline monochemotherapy for the treatment of elderly patients with acute promyelocytic leukemia
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Sanz, Miguel A., Vellenga, Edo, Rayón, Chelo, Díaz-Mediavilla, Joaquín, Rivas, Concha, Amutio, Elena, Arias, Jesús, Debén, Guillermo, Novo, Andrés, Bergua, Juan, de la Serna, Javier, Bueno, Javier, Negri, Silvia, Beltrán de Heredia, José M., and Martín, Guillermo
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- 2004
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21. TEAM Conditioning (Thiotepa, Etoposide, Cytarabine, Melphalan) Prior to Autologous Hematopoietic Stem Cell Transplantation for Hodgkin and Non-Hodgkin Lymphoma: Final Results from a Prospective Multicenter Study
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Malek Aoudjhane, Elise Corre, Laurence Heuberger, Paul Coppo, Roberta Di Blasi, Remy Dulery, Tounes Ledraa, Mohamad Mohty, Anne Vekhoff, Laure Lebras, Ahmad Al Jijakli, Zora Marjanovic, Eolia Brissot, Florent Malard, Giorgia Battipaglia, Clemence Mediavilla, Simona Lapusan, Sylvain Choquet, and Ramdane Belhocine
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Melphalan ,medicine.medical_specialty ,business.operation ,business.industry ,Immunology ,Mallinckrodt ,Cell Biology ,Hematology ,ThioTEPA ,medicine.disease ,Biochemistry ,Regimen ,International Prognostic Index ,Internal medicine ,Cytarabine ,Medicine ,business ,Diffuse large B-cell lymphoma ,Etoposide ,medicine.drug - Abstract
Introduction Although a large variety of conditioning regimens are used in autologous hematopoietic stem cell transplantation (autoSCT), including the widely used BEAM (carmustine, etoposide, cytarabine, melphalan), there is no consensus regarding a standard approach. In the context of a carmustine shortage, we replaced it with thiotepa. However, clinical data on the TEAM (thiotepa, etoposide, cytarabine, melphalan) conditioning regimen are sparse and only retrospective. Thus, we designed a multicenter prospective study (NCT02504190) to assess the efficacy and toxicity of a TEAM conditioning regimen. Patients and methods The TEAM regimen consisted of a total dose of thiotepa of 8 mg/kg on day -6; etoposide 100 mg/m2/12h and cytarabine 200 mg/m2/12h (day -5 to -2); melphalan 200 mg/m2 on day-1. Inclusion criteria were the following: age between 18 and 65 years, biopsy-proven Hodgkin or non-Hodgkin lymphoma, HIV seronegative, and first autoSCT. Results Seventy-four male and eighteen female patients with a median age of 53 years (range, 19-65) were included. Karnofsky score was Median time to neutrophil recovery was 12 days (range, 9-48) and to platelet recovery >20 G/L was 13 days (range, 7-197). The most significant regimen-related toxicities were mucositis in 100% of patients (median grade=3, range, 1-4) and diarrhea in 98% of patients (median grade=1, range, 0-3). Other non-hematologic grade 3 adverse events occurred in 17 patients (18%). Blood cultures were positive for Staphyloccocus sp. in 27% patents, other Gram- positive bacteria in 5% and Gram negative in 6%. Central line-associated bloodstream infection occurred in 22 patients (24%). Invasive fungal infection occurred in 3 patients. Four patients required transfer to the intensive care unit. The median length of stay in hospital was 27 days (range, 16-62). At day+100, 89 patients were evaluable for response and all were in CR. Deaths directly attributed to disease progression or relapse occurred in 5 patients. After a median follow-up of 31 months (range, 15-48), the non-relapse mortality (NRM) was 3.3%. Two patients died of infection during aplasia and one patient died 67 days after autoSCT of necrotizing fasciitis. At last follow-up, 17 patients (19%) relapsed, 9 died and 83 were alive. The estimated 3-year overall survival (OS) and progression-free survival (PFS) were 90.2% and 77.2%, respectively. In patients with double expressor diffuse large B-cell lymphoma (n=15), the estimated 3-year OS and PFS were 93% and 73%, respectively. None of the 31 patients with intermediate or high-risk CNS international prognostic index experienced CNS relapse. Conclusion The TEAM conditioning regimen is a safe and valid platform in autoSCT for patients with high-risk or relapsed/refractory lymphoma. Although mucositis and diarrhea were frequent, the NRM was similar to that reported for BEAM conditioning. Most notably, no CNS relapse occurred in patients at intermediate or high-risk of CNS relapse. Disclosures Duléry: Keocyt: Honoraria. Choquet:Keocyt: Honoraria. Di Blasi:Novartis: Honoraria. Malard:Therakos/Mallinckrodt: Honoraria; Janssen: Honoraria; Keocyte: Honoraria; Sanofi: Honoraria; JAZZ pharmaceutical: Honoraria; Astellas: Honoraria. Coppo:Ablynx/Sanofi: Consultancy; Shire: Consultancy; Alexion: Consultancy. Mohty:Jazz Pharmaceuticals: Honoraria, Research Funding.
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- 2019
22. Investigating Antibiotic Exposure and Risk of Severe Acute Graft Versus Host Disease in Patients Undergoing Allogeneic Hematopoietic Stem Cell Transplantation
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Tounes Ledraa, Giorgia Battipaglia, Clemence Mediavilla, Razan Mohty, Florent Malard, Agnès Bonnin, Simona Sestili, Mohamad Mohty, Eolia Brissot, Béatrice Gaugler, and Remy Dulery
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Cyclophosphamide ,Umbilical Cord Blood Transplantation ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,medicine.disease ,Biochemistry ,Transplantation ,Graft-versus-host disease ,medicine.anatomical_structure ,medicine ,Anaerobic bacteria ,Bone marrow ,Stem cell ,business ,medicine.drug - Abstract
Several studies have shown that alteration of the microbiota, particularly in the gastrointestinal tract, can be associated with graft-versus-host disease (GvHD). Patients undergoing allogeneic hematopoietic stem cell transplantation (allo-SCT) usually get exposed to antibiotics (ATB), mainly in the peri-transplant period. Moreover, ATB, specifically those that target anaerobic bacteria, can alter the microbiota in a variety of body organs. This, in turn, renders several organs vulnerable to injury making them more prone to developing diseases such as GvHD. In this study, we evaluate whether the use of ATB, characterized by duration, timing and type, in the peri-transplant period, is associated with an increased incidence of acute GvHD (aGvHD) and aGvHD-related mortality. In this retrospective study, we included 318 consecutive patients who underwent allo-SCT including haploidentical and cord blood transplantation between December 2012 and June 2018 at a single center. ATB exposure was collected and classified into 3 groups according to the date of initiation of ATB: from the start of conditioning to day - 1 of allo-SCT (early ATB exposure), from day 0 to neutrophil engraftment (late ATB exposure) and a third group of patients who did not receive ATB (no ATB exposure). ATB were only initiated if a patient develop fever or show signs of infection. Patients did not receive any prophylactic ATB. Exposure was further categorized as primary or adjunct. Primary exposure included the use of 4 classes of ATB: carbapenems, anti-pseudomonal penicillin, 4thand 5thgeneration cephalosporins and fluoroquinolones. Adjunct ATB comprised other ATB and was further divided into 2 groups according to anaerobic coverage. The median age at transplant was 55 years. The stem cell source was peripheral blood in 85%, bone marrow in 10% and cord blood in 5% of the patients. Ninety-nine percent of the patients received cyclosporine A as GvHD prophylaxis and 80% and 7% of the patients received (along with cyclosporine A), mycophenolate mofetil and methotrexate, respectively. In addition, 35% and 89% of the patients received post-transplant cyclophosphamide and anti-thymocyte globulin, respectively, as GvHD prophylaxis. The median time to neutrophil engraftment was 16 days post-transplant. The median follow-up was 85 months. 93.7% of the patients received ATB in the peri-transplant period with 64.5% of them in the early ATB exposure group, and 29.2% of them in the late ATB exposure group. The 2-year overall survival and progression free survival were 74.3% and 63.6% in patients with early ATB exposure, compared to 79.5% and 70.8% in patients with late ATB exposure (p=0.11 and p=0.07 respectively). The 2-year cumulative incidence of non-relapse mortality was 16.5% in patients with early ATB exposure, compared to 15.1% in patients with late ATB exposure (p=0.63). The 180-days cumulative incidence of grade 2-4 and 3-4 aGvHD were 23.8% and 12.2% in patients with early ATB exposure, compared to 27.2% and 5.4% in patients with late ATB exposure (p=0.64 and p=0.06 respectively). In multivariate analysis, including the most important parameters associated with GvHD (stem cell source and donors, conditioning regimen, sex mismatch and patients age), early ATB initiation was the only parameter associated with a significantly higher risk of severe grade 3-4 aGvHD [HR 0.51 (0.28-0.90); p=0.02]. In conclusion, in the absence of any ATB prophylaxis, early initiation of ATB, before graft infusion,is associated with a significantly higher risk of severe grade 3-4 aGvHD. Weighing risk of morbidity and mortality associated with infections versus later on risk of developing aGvHD is essential. Hence, new strategies should be developed to risk stratify patients with fever and thus to avoid early non necessary ATB exposure especially in those who develop fever during anti-thymocyte globulin infusion. Studies evaluating such strategies will be necessary in the next years. Disclosures Mohty: Jazz Pharmaceuticals: Honoraria, Research Funding. Malard:Astellas: Honoraria; JAZZ pharmaceutical: Honoraria; Sanofi: Honoraria; Keocyte: Honoraria; Janssen: Honoraria; Therakos/Mallinckrodt: Honoraria.
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- 2019
23. Extracorporeal Photopheresis for First Line Treatment of Acute Graft Versus Host Disease
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Malard, Florent, primary, Sestili, Simona, additional, Eder, Sandra, additional, Belhocine, Ramdane, additional, Ruggeri, Annalisa, additional, Battipaglia, Giorgia, additional, Brissot, Eolia, additional, Mediavilla, Clemence, additional, Dulery, Remy, additional, and Mohty, Mohamad, additional
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- 2018
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24. Acute Myeloblastic Leukemia Relapse after Allogeneic Stem Cell Transplantation
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Clémence, Mediavilla, primary, Vigouroux, Stéphane, additional, Tabrizi, Reza, additional, Clement, Laurence, additional, Banos, Anne, additional, Turlure, Pascal, additional, Lafarge, Xavier, additional, Dumas, Pierre-Yves, additional, Leguay, Thibaut, additional, Pigneux, Arnaud, additional, Milpied, Noel, additional, and Forcade, Edouard, additional
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- 2018
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25. A Delayed Innate T Cell Reconstitution Is Associated with Epstein-Barr Virus Reactivation after Haploidentical Hematopoietic Stem Cell Transplantation Using Anti-Thymoglobulin and High-Dose Post-Transplant Cyclophosphamide
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Stocker, Nicolas, primary, Farge, Agathe, additional, Ricard, Laure, additional, Jachiet, Vincent, additional, Mediavilla, Clemence, additional, Brissot, Eolia, additional, Battipaglia, Giorgia, additional, Duléry, Remy, additional, Sestili, Simona, additional, Ruggeri, Annalisa, additional, Mohty, Razan, additional, Mohty, Mohamad, additional, Gaugler, Béatrice, additional, and Malard, Florent, additional
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- 2018
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26. Achievement of High Concentration of Cyclosporine-a Is Associated with a Low Incidence of Acute Graft-Versus-Host Disease after Haploidentical Hematopoietic Cell Transplantation Using Post-Transplant Cyclophosphamide and Peripheral Blood Stem Cell Graft
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Stocker, Nicolas, primary, Duléry, Remy, additional, Mediavilla, Clemence, additional, Brissot, Eolia, additional, Battipaglia, Giorgia, additional, Sestili, Simona, additional, Ruggeri, Annalisa, additional, Mohty, Razan, additional, Mohty, Mohamad, additional, and Malard, Florent, additional
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- 2018
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27. Hematopoietic Recovery and Transfusion Needs after Haploidentical Stem Cell Transplantation with Post-Transplant Cyclophosphamide in Adult Patients with Hematologic Malignancies
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Mohty, Razan, primary, Malard, Florent, additional, Ruggeri, Annalisa, additional, Brissot, Eolia, additional, Dulery, Remy, additional, Battipaglia, Giorgia, additional, Giannotti, Frederica, additional, Sestili, Simona, additional, Mediavilla, Clemence, additional, and Mohty, Mohamad, additional
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- 2018
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28. Early immunophenotypical evaluation of minimal residual disease in acute myeloid leukemia identifies different patient risk groups and may contribute to postinduction treatment stratification
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San Miguel, Jesús F., Vidriales, Marı́a B., López-Berges, Consuelo, Dı́az-Mediavilla, Joaquı́n, Gutiérrez, Norma, Cañizo, Consuelo, Ramos, Fernando, Calmuntia, Marı́a J., Pérez, José J., González, Marcos, and Orfao, Alberto
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- 2001
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29. Definition of relapse risk and role of nonanthracycline drugs for consolidation in patients with acute promyelocytic leukemia: a joint study of the PETHEMA and GIMEMA cooperative groups: Presented in part at the 41st meeting of the American Society of Hematology, New Orleans, LA, December 3-7, 1999.
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Sanz, Miguel A., Coco, Francesco Lo, Martı́n, Guillermo, Avvisati, Giuseppe, Rayón, Consuelo, Barbui, Tiziano, Dı́az-Mediavilla, Joaquı́n, Fioritoni, Giuseppe, González, José David, Liso, Vincenzo, Esteve, Jordi, Ferrara, Felicetto, Bolufer, Pascual, Bernasconi, Carlo, Gonzalez, Marcos, Rodeghiero, Francesco, Colomer, Dolors, Petti, Maria C., Ribera, José M., and Mandelli, Franco
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- 2000
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30. Hematopoietic Recovery and Transfusion Needs after Haploidentical Stem Cell Transplantation with Post-Transplant Cyclophosphamide in Adult Patients with Hematologic Malignancies
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Razan Mohty, Clemence Mediavilla, Eolia Brissot, Annalisa Ruggeri, Florent Malard, Simona Sestili, Mohamad Mohty, Giorgia Battipaglia, Frederica Giannotti, and Remy Dulery
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Oncology ,medicine.medical_specialty ,Blood transfusion ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,medicine.disease ,Biochemistry ,Transplantation ,Haematopoiesis ,medicine.anatomical_structure ,Graft-versus-host disease ,Internal medicine ,medicine ,Bone marrow ,Stem cell ,business ,medicine.drug - Abstract
Introduction: Haploidentical stem cell transplantation (Haplo-SCT) using post-transplant cyclophosphamide is widely used for the treatment of patients with hematologic malignancies especially in patients who lack related or unrelated donors. Several factors were shown to affect hematopoietic recovery after allogeneic hematopoietic stem cell transplantation, including age at transplant, initial diagnosis, stem-cell source, CD34 count in the graft, conditioning regimen intensity, infections, etc. The aim of this study was to evaluate immune reconstitution, neutrophils and platelets recovery, transfusion needs and main transplant outcomes after Haplo-SCT and to identify factors correlated with improved or worsened recovery. Methods: This study included 72 consecutive patients who underwent haploidentical stem cell transplantation between December 2012 and March 2018, in a single center. Graft was either bone marrow (n=21) or peripheral blood stem cells (n=51). Patients were given PT-Cy (50mg/kg/d) for one (n=20) or two days (n=52), cyclosporine, mycophenolate mofetil and ATG for GvHD prophylaxis. Conditioning regimen was reduced toxicity one (RIC) in 69% (n=50) of patients, and a myeloablative one (MAC) in 31% (n=22) of cases . Main transplant outcomes, neutrophil and platelets recovery, and needs for transfusion were evaluated at day +30, day +60 and day +90 after Haplo-SCT. Results: The median CD34+ cells infused was 10.62 x106/kg (range, 1.02-15.06). The median time to neutrophil recovery (>500/µL) was 16 days post-transplant (range, 5-29). The median time to neutrophil count >1000/µL was 17 days post-transplant (range, 5-32). Platelets recovery (>20.000/µL) was observed at a median of 13 days post-transplant (range, 10-193). Platelets recovery (>50.000/µL) was documented at a median of 60 days post-transplant (range, 10-193). Forty-five (63%) and 49 (68%) patients had a platelets count >50.000/µL at days +30 and +60 post-transplant respectively. At day +90 post-transplant, 61 patients were assessable with 56 (92%) of them having a platelets count >50.000/µL. In this series, 61 patients (85%) needed platelets transfusion with a median number of 13 units (range, 2-60), transfused for a median of 26 days (range, 5-198) post-transplant. Similarly, 59 patients (82%) needed RBCs transfusion and received a median of 6 RBCs packs (range, 1-60) for a median duration of 31 days (range, 5-147) post-transplant. 23 patients (32%) had anemia and/or thrombocytopenia requiring the administration of growth factors, either erythropoietin in 14 patients (19%) (Epoetin, n=8 or Darbepoetin, n=6) and/or Thrombopoietin in 15 patients (20%) (Romiplostim, n=13 or Eltrombopag, n=2). In all, 6 patients received unmanipulated CD34+ donor stem cell ("boost" procedure), 5 of them for poor graft function and 1 patient as a consolidation after immunosuppressive therapy for relapse after Haplo-SCT. Such boost proved to be successful in 4 patients while 2patients had an incomplete response with persistent anemia and/or thrombocytopenia. In the multivariable analysis, donor age (>40 years) and CMV reactivation within the first 3 months were shown to meaningfully affect day +90 platelets recovery >100 x109/L (HR 4.86, 95%CI 1.08-21.80, p=0.04 and HR 5.15, 95% CI, 1.22-21.7, p=0.03). Number of CD34, conditioning regimen, stem cell source, donor age and disease status at transplant have no impact on platelet recovery at day +90. Conclusion: In conclusion, our data show that Haplo-SCT is associated with a fast hematopoietic recovery with an acceptable rate of transfusions. While the adverse impact of CMV reactivation on platelet recovery was expected, the relation between donor age (>40 years) and platelets recovery is new. This finding should guide in selection of potential donors for Haplo-SCT. Disclosures Mohty: Celgene: Consultancy, Honoraria; Jazz Pharmaceuticals: Honoraria, Research Funding, Speakers Bureau; Amgen: Consultancy, Honoraria; Takeda: Honoraria, Speakers Bureau; Janssen: Honoraria, Research Funding, Speakers Bureau; Servier: Consultancy; MaaT Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees; Molmed: Consultancy; Bristol Myers: Consultancy, Research Funding; Sanofi: Consultancy, Honoraria, Research Funding, Speakers Bureau.
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- 2018
31. Acute Myeloblastic Leukemia Relapse after Allogeneic Stem Cell Transplantation
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Reza Tabrizi, Thibaut Leguay, Pierre-Yves Dumas, Edouard Forcade, Xavier Lafarge, Anne Banos, Stephane Vigouroux, Mediavilla Clémence, Pascal Turlure, Laurence Clement, Arnaud Pigneux, and Noel Milpied
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Univariate analysis ,medicine.medical_specialty ,Performance status ,business.industry ,medicine.medical_treatment ,Immunology ,Immunosuppression ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,medicine.disease ,Biochemistry ,Chemotherapy regimen ,Donor lymphocyte infusion ,Transplantation ,Graft-versus-host disease ,Internal medicine ,medicine ,business - Abstract
Allogeneic haematopoietic stem cell transplantation (HSCT) is a curative option for acute myeloid leukaemia (AML). A major improvement of conditioning regimen has been realized in the last two decades, offering the opportunity for older patients to undergo HSCT with an acceptable toxicity profile. Unfortunately, relapse remains the main cause of death, and only few studies analyzed the survival of patients presenting with post-HSCT AML relapse and their treatment options. The purpose of this study was to analyze the survival of patients with post-HSCT AML relapse, to describe the treatment options and to search for factors associated with poor prognosis at relapse. In this study, we collected the data of all the patients transplanted between January 2005 and December 2014 at Bordeaux university centre and showing AML relapse after HSCT. Between 2005 and 2014, out of 312 HSCT for AML, one hundred patients relapsed at our center with a median time from transplant of 123.5 days (11-2726 days). Median age was 55 years old (range 17-65), 44 patients were male. Donors were matched related for 49 patients, unrelated for 35 patients, and cord blood units were used in 16 patients. Conditioning regimen was reduced for 70 patients and myeloablative for 30 patients. According to the Disease Risk Index, 4 patients were considered at low risk, 29 patients at intermediate risk, 43 patients at high risk and 11 patients at very high risk. Thirty one per cent of patients had refractory disease. Before relapse, 31 patients developed Acute Graft Versus Host Disease (GVHD) and 9 patients developed Chronic GVHD. At relapse, 62 patients were still on cyclosporine and 22 on steroids. Eighty four patients presented an isolated bone marrow relapse, while 5 patients showed isolated extramedullary relapse, and 11 mixed relapse. With a median follow-up from relapse of 106 days (0-3619 days), the 1 and 2-year overall survival (OS) were 24% and 13%, respectively. At final follow-up 8 patients were still alive. For alive patients, median follow-up from relapse was 1524 days (980-3619 days). Median age was 39 years old (20-57 years old), DRI was considered at intermediate risk for 2 patients, at high risk for 5 patients and at very high risk for 1 patient. No patient was FLT3 mutation. In univariate analysis, factors associated with better OS at relapse were age < 45 years old, male gender, performance status at relapse > 70%, and no initial FLT3 mutation. Male gender, performance status at relapse, early relapse and no initial FLT3 mutation were associated with a better OS in multivariate analysis. Seven patients responded to immunosuppression tapering and 19 patients to first line treatment containing local radiotherapy, chemotherapy and/or Donor Lymphocyte Infusion (DLI). Developing GVHD after immunosuppression tapering or DLI was associated to disease response: Seven patients responded after immunosuppression only of whom 4 after developing GVHD (p=0.0013). Twelve patients responded after DLI of whom 7 after developing GVHD (p=0.05). Patients receiving an association of chemotherapy and DLI showed a better response and a better survival compared to chemotherapy only (p= 0.03). Patients with FLT3 mutation did not respond to any treatment. This study confirms the severity of AML relapse after HSCT with a poor long term OS. The particularly poor impact of FL3 mutation suggests the use of targeted therapy in a prophylactic setting. Immunomodulatory approaches resulted in disease response in some patients and should be evaluated prospectively to identify clinical and biological factors predictive of the response. Disclosures No relevant conflicts of interest to declare.
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- 2018
32. Achievement of High Concentration of Cyclosporine-a Is Associated with a Low Incidence of Acute Graft-Versus-Host Disease after Haploidentical Hematopoietic Cell Transplantation Using Post-Transplant Cyclophosphamide and Peripheral Blood Stem Cell Graft
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Eolia Brissot, Annalisa Ruggeri, Simona Sestili, Florent Malard, Mohamad Mohty, Razan Mohty, Clemence Mediavilla, Giorgia Battipaglia, Remy Dulery, and Nicolas Stocker
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medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,medicine.disease ,Biochemistry ,Gastroenterology ,Granulocyte colony-stimulating factor ,Fludarabine ,Transplantation ,Graft-versus-host disease ,medicine.anatomical_structure ,Internal medicine ,medicine ,Bone marrow ,Stem cell ,business ,Busulfan ,medicine.drug - Abstract
Background: Allogeneic hematopoietic stem cell transplantation using a related haploidentical donor (Haplo-HCT) using post-transplant high-dose cyclophosphamide (PTCy) is increasingly used for patients lacking a matched related or unrelated donor. However, use ofperipheral blood stem cell graft (PBSC) is associated with an increased risk of acute GvHD (aGvHD) compared to bone-marrow graft. Therefore, while cyclosporine A (CsA) and mycophenolate mofetil (MMF) are traditionally initiated after completion of PT-Cy at day +5, we decided to initiate them at day -3 before transplant and to add a low dose of ATG to reinforce GvHD prophylaxis in those patients. With this background, we analyze retrospectively the impact of early initiation of CsA and of CsA concentration on patients' outcome in all patients who underwent Haplo-HCT with PBSC grafts and PTCy. Patients and Methods: Sixty-one consecutive patients who underwent Haplo-HCT for hematological malignancies between October 2013 and August 2017 were included in this retrospective single-center study. All patients received G-CSF mobilized PBSC as grafts and post-transplantation immunosuppression with CsA and MMF. CsA was administered at a dose of 3mg/kg by continuous intravenous infusion starting from D -3 and changed to twice daily oral dosing as soon as tolerated.MMF was administered at a fixed oral dose of 2g per day starting from day 6 without adjustment. In the absence of GvHD, MMF and CsA were tapered over 4 weeks starting from day 30 and day 60, respectively. CsA blood trough concentrations were monitored 3 times per week during the intravenous treatment and at least once per week after switch to oral dosing. CsA doses were adjusted to achieve blood levels between 200 and 300 ng/mL and to prevent renal dysfunction. The primary endpoint was to determine the impact of the CsA concentration on the risk of grade II-IV and III-IV aGvHD. Results: Median age was 53 (range, 15-72) years, with 16 male patients (26%) receiving a graft from a female donor. Diagnoses were myeloid (64%) or lymphoid malignancies (36%). According to the Disease Risk Index, patients were considered as low-risk, intermediate-risk, high-risk or very-high-risk (respectively 8%, 56%, 31% and 5%). Twenty-five patients (41%) with refractory disease received a sequential conditioning regimen while the remaining (n=36, 59%) received a RIC/RTC regimen based on fludarabine, busulfan and thiotepa. 51 patients (83%) received ATG (2.5-5 mg/Kg total dose) as part of the conditioning regimen. All patients received standard PTCy, nine at D+3 (15%) and 52 at D+3 and D+5 (85%). All patients engrafted at a median of 18 (range: 13-35) days after Haplo-HCT and the median follow-up among surviving patients was 21 (range: 13-53) months. The median concentrations of CsA at 1, 2, 3, and 4 weeks after Haplo-HCT were 272 (range: 114-911), 296 (range: 132-516), 251 (range: 111-485), and 246 (range: 36-375) ng/mL, respectively. At d180, the cumulative incidences (CIs) of grade II-IV and grade III-IV aGvHD were 39% and 18%, respectively. The CIs of chronic GvHD (cGvHD), extensive cGvHD and relapse were 41%, 19% and 35% at 18 months after Haplo-HCT, respectively. At 18 months after the transplant, the OS, PFS and GPFS rates were respectively 60%, 55% and 48%. In univariate analysis, patients having the lowest CsA concentration in the first week after Haplo-HCT had a significantly higher risk of grade II-IV aGvHD (49% vs 18%; P= .02), severe grade III-IV aGvHD (26% vs 0%; P = .03), cGvHD (P= .02) and extensive cGvHD (P= .04). We do not find statistically significant correlation between CsA concentration and relapse incidence, NRM, PFS, GPFS or OS. In multivariate logistic regression analysis, higher CsA concentration (> 301 ng/ mL; the cut-off value defined by ROC analysis) during the first week following Haplo-HCT was the only independent parameter significantly associated with a reduced risk of grade II-IV and grade III-IV aGvHD (respectively P = .04; RR .11; 95% CI, 0.05-0.94; and P < .0001; RR < .001; 95% CI, 0.000007-0.00006). There was no association with extensive cGvHD (P = .14; RR .11; 95% CI, 0.06-1.48). Conclusion: We conclude that achievement of high concentration of CsA early after Haplo-HCT using PBSC graft is associated with a low incidence of aGvHD and that CsA should be initiated at time of transplant with adequate monitoring during the engraftment period to reduce the risk of grade II-IV aGvHD. Disclosures Mohty: MaaT Pharma: Consultancy, Honoraria.
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- 2018
33. Extracorporeal Photopheresis for First Line Treatment of Acute Graft Versus Host Disease
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Florent Malard, Simona Sestili, Remy Dulery, Giorgia Battipaglia, Annalisa Ruggeri, Sandra Eder, Mohamad Mohty, Clemence Mediavilla, Ramdane Belhocine, and Eolia Brissot
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medicine.medical_specialty ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,Immunology ,Salvage therapy ,Cell Biology ,Hematology ,medicine.disease ,Single Center ,Biochemistry ,Transplantation ,Regimen ,Graft-versus-host disease ,Photopheresis ,Internal medicine ,medicine ,Cumulative incidence ,business ,medicine.drug - Abstract
Background. Extracorporeal photopheresis (ECP) is increasingly used in the treatment of acute and chronic graft versus host disease (aGVHD). It is recognized as an immunomodulatory therapy that can decrease inflammation and allow for immune tolerance. Interesting results have obtained using ECP as salvage therapy in patients with steroid-refractory GVHD after failure of one or more lines of immunosuppressive therapy. With this background, we hypothesized that incorporation of ECP as part of first line treatment for aGVHD may improve response rate and avoid the development of steroid-refractory or steroid-dependent aGVHD, mainly in cases of aGVHD with predominant skin involvement. Patients and methods. Forty consecutive patients who received ECP as part of their first line treatment of aGVHD with skin involvement between 2013 and 2016 were included in this single center analysis. 36 patients (90%) received granulocyte-colony stimulating factor mobilized PBSC as grafts, 3 patients (7.5%) received a bone marrow graft and one patient (2.5%) received an umbilical cord blood graft. Donor was a matched related one for 10 patients, haploidentical for 8 patients, matched unrelated for 16 patients and mismatched unrelated for 6 patients. 4 patients received a reduced intensity conditioning regimen and 36 a myeloablative reduced toxicity conditioning regimen. All patients received a combination of cyclosporine A (CsA) and mycophenolate mofetil for GVHD prophylaxis, except for patients with a matched related donor who received cyclosporine A alone. Patients transplanted with a haploidentical donor, received post-transplant cyclophosphamide (PTCy; 50mg/kg/d at d+3/d+5). ECP was initiated as soon as possible after the diagnosis of aGVHD with predominant skin involvement. The primary endpoint was to determine the best response achieved through the use of ECP for first line treatment of aGVHD. Results. Median age was 57 (range, 22-66) years, with 28 male patients (49%). Diagnoses were myeloid (n=32) or lymphoid malignancies (n=8) and disease status at transplant was complete remission in 23 patients, partial remission in 8 patients, relapse/progression in 8 patients and untreated in 2 patients. Patients developed aGVHD at a median of 31 days after alloHCT (range, 11-129). 5 patients had late onset aGVHD and two patients developed aGVHD after DLI. None of them had an overlap syndrome. aGVHD grade was I in 13 patients, II in 18 patients and III-IV in 9 patients. All patients had skin involvement and 10 patients had gut (n=8) and/or liver (n=5) involvement. All patients, but one, were still receiving CsA at time of aGVHD diagnosis. Systemic corticosteroids were initiated in 29 patients, while 11 patients received only topical dermo-corticoids. ECP was initiated at a median of 9 days (range, 0-83) after the diagnosis of aGVHD. ECP was performed weekly for 26 patients and twice weekly for 14 patients. Overall response rate (ORR) was 80% after a median of 3 (range, 1-9) weeks of treatment, including 24 complete responses (CR, 60%), 6 very good partial responses (VGPR, 15%) and 2 partial responses (PR, 5%). According to aGVHD grade, in patients with grade I aGVHD, the ORR was 92%, including 11 CR and 1 VGPR. In patients with grade II aGVHD, the ORR was 83%, including 8 CR and 7 VGPR/PR. In patients with grade III-IV aGVHD, ORR was 55%, all of them being in CR. In responding patients, 1 patient presented a recurrence of aGVHD and 15 developed chronic GVHD, including 4 overlap syndromes. Median follow-up was 32 months (range, 22-58) among surviving patients. At 24 months after transplant, the OS and PFS rates were respectively 78% (95% CI, 61%-88%) and 56% (range, 39%-70%). The 2-years cumulative incidence of relapse was 33% (95% CI, 19%-49%) and the 2-years cumulative incidence of non-relapse mortality was 10% (95% CI, 3%-22%). Conclusion. ECP is an effective and well-tolerated option for treatment of acute GVHD with predominant skin involvement with a 2 years cumulative incidence of NRM of 10% and an ORR of 80%. Best responses were seen in patients with grade I-II aGVHD, suggesting that ECP should be started as early as possible after the diagnosis of aGVHD. Incorporation of ECP as part of first line treatment for aGVHD may improve response rate and avoid the development of steroid-refractory aGVHD. Prospective randomized trial are warranted to evaluate ECP as adjuvant treatment for first line treatment of skin aGVHD. Disclosures Mohty: Celgene: Consultancy, Honoraria; Takeda: Honoraria, Speakers Bureau; Janssen: Honoraria, Research Funding, Speakers Bureau; Jazz Pharmaceuticals: Honoraria, Research Funding, Speakers Bureau; MaaT Pharma: Consultancy, Membership on an entity's Board of Directors or advisory committees; Bristol Myers: Consultancy, Research Funding; Sanofi: Consultancy, Honoraria, Research Funding, Speakers Bureau; Molmed: Consultancy; Servier: Consultancy; Amgen: Consultancy, Honoraria.
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- 2018
34. A Modified AIDA Protocol With Anthracycline-Based Consolidation Results in High Antileukemic Efficacy and Reduced Toxicity in Newly Diagnosed PML/RAR∝-Positive Acute Promyelocytic Leukemia: Presented in part at the 40th meeting of the American Society of Hematology, Miami Beach, FL, December 4-8, 1998.
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Sanz, Miguel A., Martín, Guillermo, Rayón, Consuelo, Esteve, Jordi, González, Marcos, Díaz-Mediavilla, Joaquín, Bolufer, Pascual, Barragán, Eva, Terol, María J., González, José D., Colomer, Dolors, Chillón, Carmen, Rivas, Concha, Gómez, Teresa, Ribera, José M., Bornstein, Rafael, Román, José, Calasanz, María J., Arias, Jesus, Álvarez, Carmen, Ramos, Fernando, and Debén, Guillermo
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- 1999
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35. Differentiation syndrome in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and anthracycline chemotherapy
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Bob Löwenberg, Guillermo Deben, Silvia Negri, Miguel A. Sanz, José D. González, Marcos González, Angel Leon, Salut Brunet, Joaquín Díaz-Mediavilla, Javier de la Serna, Mar Tormo, Chelo Rayon, Elena Amutio, Ricardo Parody, Juan Bergua, Jordi Esteve, Edo Vellenga, Gustavo Milone, Pau Montesinos, Concha Rivas, Hematology, Faculteit Medische Wetenschappen/UMCG, Guided Treatment in Optimal Selected Cancer Patients (GUTS), and Stem Cell Aging Leukemia and Lymphoma (SALL)
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Male ,Time Factors ,medicine.medical_treatment ,MULTICENTER ,Biochemistry ,Gastroenterology ,MOLECULES ,Leukemia, Promyelocytic, Acute ,Recurrence ,Risk Factors ,Anthracyclines ,Child ,CONSOLIDATION ,Aged, 80 and over ,RISK ,Syndrome ,Hematology ,Middle Aged ,Prognosis ,CYTARABINE ,Leukemia ,Child, Preschool ,Drug Therapy, Combination ,Female ,medicine.drug ,Adult ,Acute promyelocytic leukemia ,medicine.medical_specialty ,Adolescent ,Immunology ,Tretinoin ,ACUTE MYELOID-LEUKEMIA ,Disease-Free Survival ,Internal medicine ,medicine ,MANAGEMENT ,Humans ,Idarubicin ,Aged ,Chemotherapy ,business.industry ,Cell Biology ,REMISSION ,medicine.disease ,Mercaptopurine ,RANDOMIZED-TRIAL ,Retinoic acid syndrome ,IDARUBICIN ,Cytarabine ,business - Abstract
Differentiation syndrome (DS) can be a life-threatening complication in patients with acute promyelocytic leukemia (APL) undergoing induction therapy with all-trans retinoic acid ( ATRA). Detailed knowledge about DS has remained limited. We present an analysis of the incidence, characteristics, prognostic factors, and outcome of 739 APL patients treated with ATRA plus idarubicin in 2 consecutive trials (Programa Espanol de Tratamientos en Hematologa [PETHEMA] LPA96 and LPA99). Overall, 183 patients (24.8%) experienced DS, 93 with a severe form (12.6%) and 90 with a moderate form (12.2%). Severe but not moderate DS was associated with an increase in mortality. A bimodal incidence of DS was observed, with peaks occurring in the first and third weeks after the start of ATRA therapy. A multivariate analysis indicated that a WBC count greater than 5 x 10(9)/L and an abnormal serum creatinine level correlated with an increased risk of developing severe DS. Patients receiving systematic prednisone prophylaxis (LPA99 trial) in contrast to those receiving selective prophylaxis with dexamethasone (LPA96 trial) had a lower incidence of severe DS. Patients developing severe DS showed a reduced 7-year relapse-free survival in the LPA96 trial (60% vs 85%, P = .003), but this difference was not apparent in the LPA99 trial (86% vs 88%). (Blood. 2009; 113: 775-783)
- Published
- 2009
36. Causes and prognostic factors of remission induction failure in patients with acute promyelocytic leukemia treated with all-trans retinoic acid and idarubicin
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Miguel A. Sanz, Pau Montesinos, Javier de la Serna, Elena Amutio, Ricardo Parody, Silvia Negri, Joaquín Díaz-Mediavilla, Marcos González, Bob Löwenberg, Angel Leon, Chelo Rayon, Salut Brunet, Mar Tormo, Juan Bergua, Concha Rivas, Jordi Esteve, José González, Guillermo Deben, Edo Vellenga, Gustavo Milone, Faculteit Medische Wetenschappen/UMCG, Guided Treatment in Optimal Selected Cancer Patients (GUTS), Stem Cell Aging Leukemia and Lymphoma (SALL), and Hematology
- Subjects
Male ,ANTHRACYCLINE MONOCHEMOTHERAPY ,THERAPY ,Biochemistry ,Gastroenterology ,Leukemia, Promyelocytic, Acute ,Risk Factors ,Induction Death ,Antineoplastic Combined Chemotherapy Protocols ,Treatment Failure ,Child ,Aged, 80 and over ,Hematology ,Remission Induction ,Age Factors ,Syndrome ,Middle Aged ,Survival Rate ,Leukemia ,Child, Preschool ,Creatinine ,Female ,medicine.drug ,Adult ,Acute promyelocytic leukemia ,medicine.medical_specialty ,Adolescent ,Immunology ,Hemorrhage ,Tretinoin ,Infections ,Disease-Free Survival ,Sex Factors ,Internal medicine ,Coagulopathy ,medicine ,Humans ,Idarubicin ,Survival rate ,Aged ,business.industry ,MOLECULAR REMISSION ,Cell Biology ,medicine.disease ,EXPERIENCE ,Blast Crisis ,business - Abstract
An understanding of the prognostic factors associated with the various forms of induction mortality in patients with acute promyelocytic leukemia (APL) has remained remarkably limited. This study reports the incidence, time of occurrence, and prognostic factors of the major categories of induction failure in a series of 732 patients of all ages (range, 2-83 years) with newly diagnosed APL who received all-trans retinoic acid (ATRA) plus idarubicin as induction therapy in 2 consecutive studies of the Programa de Estudio y Tratamiento de las Hemopatias Malignas (PETHEMA) Group. Complete remission was attained in 666 patients (91%). All the 66 induction failures were due to induction death. Hemorrhage was the most common cause of induction death (5%), followed by infection (2.3%) and differentiation syndrome (1.4%). Multivariate analysis identified specific and distinct pretreatment characteristics to correlate with an increased risk of death caused by hemorrhage (abnormal creatinine level, increased peripheral blast counts, and presence of coagulopathy), infection (age >60 years, male sex, and fever at presentation), and differentiation syndrome (Eastern Cooperative Oncology Group [ECOG] score >1 and low albumin levels), respectively. These data furnish clinically relevant information that might be useful for designing more appropriately risk-adapted treatment protocols aimed at reducing the considerable problem of induction mortality in APL.
- Published
- 2008
37. Early Post Transplant Organ Toxicity after Allogeneic One-Haplotype Mismatch (Haplo) Hematopoietic Stem Cell Transplant (HSCT)
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Blaise, Didier, primary, Mediavilla, Clemence, additional, Meskine, Amir, additional, Bramanti, Stéfania, additional, Harbi, Samia, additional, Furst, Sabine, additional, Faucher, Catherine, additional, Granata, Angela, additional, Legrand, Faezeh, additional, Mokart, Djamel, additional, Weiller, Pierre Jean, additional, Chabannon, Christian, additional, Rey, Jérome, additional, Coso, Diane, additional, Castagna, Luca, additional, and Devillier, Raynier, additional
- Published
- 2016
- Full Text
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38. Bortezomib plus melphalan and prednisone in elderly untreated patients with multiple myeloma: results of a multicenter phase 1/2 study
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Norma-C. Gutiérrez, J Hernández, Javier de la Rubia, Dixie-Lee Esseltine, Felipe Prosper, M. Fuertes, Albert Oriol, Miguel-T Hernandez, Paz Ribas, Maria-Victoria Mateos, J.J. Lahuerta, Ramón García-Sanz, Joaquín Díaz-Mediavilla, Luis Palomera, María-José Terol, Joan Bladé, Jesús-F San Miguel, Helgi van de Velde, Adrian Alegre, José García-Laraña, Felipe de Arriba, G. Mateo, Dolores Carrera, Ana Sureda, and Joan Bargay
- Subjects
Melphalan ,medicine.medical_specialty ,Antineoplastic Agents, Hormonal ,Maximum Tolerated Dose ,Immunology ,Antineoplastic Agents ,Biochemistry ,Gastroenterology ,Disease-Free Survival ,Immunophenotyping ,Bortezomib ,Cohort Studies ,Prednisone ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Antineoplastic Agents, Alkylating ,Multiple myeloma ,Aged ,Aged, 80 and over ,business.industry ,Cell Biology ,Hematology ,medicine.disease ,Boronic Acids ,Surgery ,Transplantation ,Clinical trial ,Peripheral neuropathy ,Pyrazines ,Ciencias de la Salud [Materias Investigacion] ,Multiple Myeloma ,business ,medicine.drug - Abstract
Standard first-line treatment for elderly multiple myeloma (MM) patients ineligible for stem cell transplantation is melphalan plus prednisone (MP). However, complete responses (CRs) are rare. Bortezomib is active in patients with relapsed MM, including elderly patients. This phase 1/2 trial in 60 untreated MM patients aged at least 65 years (half older than 75 years) was designed to determine dosing, safety, and efficacy of bortezomib plus MP (VMP). VMP response rate was 89%, including 32% immunofixation-negative CRs, of whom half of the IF– CR patients analyzed achieved immunophenotypic remission (no detectable plasma cells at 10–4 to 10–5 sensitivity). VMP appeared to overcome the poor prognosis conferred by retinoblastoma gene deletion and IgH translocations. Results compare favorably with our historical control data for MP—notably, response rate (89% versus 42%), event-free survival at 16 months (83% versus 51%), and survival at 16 months (90% versus 62%). Side effects were predictable and manageable; principal toxicities were hematologic, gastrointestinal, and peripheral neuropathy and were more evident during early cycles and in patients aged 75 years or more. In conclusion, in elderly patients ineligible for transplantation, the combination of bortezomib plus MP appears significantly superior to MP, producing very high CR rates, including immunophenotypic CRs, even in patients with poor prognostic features.
- Published
- 2006
39. High-dose therapy intensification compared with continued standard chemotherapy in multiple myeloma patients responding to the initial chemotherapy: long-term results from a prospective randomized trial from the Spanish cooperative group PETHEMA
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M. Fontanillas, José García-Laraña, Alfons Soler, Manel Callís, J. Fernández-Calvo, Jesús F. San Miguel, M. Jesús Moro, Laura Rosiñol, Ana Sureda, M. Victoria Mateos, Joan Bladé, Pilar Giraldo, Santiago Gardella, Luis Palomera, Felix Carbonell, Joaquín Díaz-Mediavilla, Josep M. Ribera, Josep Martí, Programa para el Estudio de la Terapéutica en Hemopatía Maligna, Abelardo Bárez, Llorenç Font, and Jesús Trujillo
- Subjects
Male ,Melphalan ,Vincristine ,medicine.medical_specialty ,Cyclophosphamide ,medicine.medical_treatment ,Immunology ,Urology ,Biochemistry ,Dexamethasone ,law.invention ,Randomized controlled trial ,Prednisone ,law ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Multiple myeloma ,Chemotherapy ,Dose-Response Relationship, Drug ,business.industry ,Remission Induction ,Cell Biology ,Hematology ,Total body irradiation ,medicine.disease ,Carmustine ,Survival Analysis ,Surgery ,Treatment Outcome ,Doxorubicin ,Spain ,Female ,Multiple Myeloma ,business ,medicine.drug - Abstract
The aim of the present randomized trial was to compare high-dose therapy (HDT) with continued conventional chemotherapy in patients with multiple myeloma (MM) who responded to the initial treatment. From May 1994 to October 1999, 216 patients (122 men/94 women; stage II or III; Eastern Cooperative Oncology Group [ECOG] score less than 3) entered the study. Initial chemotherapy consisted of 4 cycles of alternating vincristine, BCNU, melphalan, cyclophosphamide, prednisone/vincristine, BCNU, Adriamycin, dexamethasone (VBMCP/VBAD). Responding patients were randomly assigned to receive 8 additional cycles of VBMCP/VBAD, intensification with melphalan 200 mg/m2, or melphalan 140 mg/m2 plus 12 Gy fractionated total body irradiation (TBI). One-hundred sixty-four patients were randomly assigned, 83 to continued chemotherapy and 81 to HDT. The complete remission (CR) rate was significantly higher with HDT (30% vs 11%; P = .002). However, progression-free survival (PFS) was not significantly different between HDT and conventional therapy (median, 42 vs 33 months; P = not significant [NS]), and overall survival (OS) was similar in both groups (median, 61 vs 66 months). Finally, survival after relapse was identical in the 2 arms (15.9 vs 16.4 months). In conclusion, these results show that HDT intensification, when given to myeloma patients who have responded to the initial chemotherapy, significantly increases the CR rate but has no significant impact on PFS or OS.
- Published
- 2005
40. Efficacy and Safety of Fedratinib in Patients with Myelofibrosis Previously Treated with Ruxolitinib: Results from the Phase 3 Randomized FREEDOM2 Study
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Harrison, Claire N, Mesa, Ruben, Talpaz, Moshe, Al-Ali, Haifa Kathrin, Xicoy, Blanca, Passamonti, Francesco, Palandri, Francesca, Benevolo, Giulia, Vannucchi, Alessandro M., Mediavilla, Clemence, Iurlo, Alessandra, Kim, InHo, Brown, Patrick, Hernandez, Christopher, Rose, Shelonitda, Wang, Jia, and Kiladjian, Jean-Jacques
- Abstract
Introduction
- Published
- 2023
- Full Text
- View/download PDF
41. All-trans retinoic acid and anthracycline monochemotherapy for the treatment of elderly patients with acute promyelocytic leukemia
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Javier Bueno, Andrés Novo, Javier de la Serna, Chelo Rayon, Elena Amutio, J. Arias, Jose Maria J.B. Beltran de Heredia, Miguel A. Sanz, Concha Rivas, Joaquín Díaz-Mediavilla, Guillermo Martin, Juan Bergua, Edo Vellenga, Guillermo Deben, Silvia Negri, Faculteit Medische Wetenschappen/UMCG, Guided Treatment in Optimal Selected Cancer Patients (GUTS), and Stem Cell Aging Leukemia and Lymphoma (SALL)
- Subjects
Male ,Acute promyelocytic leukemia ,Oncology ,medicine.medical_specialty ,Anthracycline ,medicine.medical_treatment ,Immunology ,MULTICENTER ,Tretinoin ,Biochemistry ,THERAPY ,Sex Factors ,Leukemia, Promyelocytic, Acute ,Maintenance therapy ,Recurrence ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Idarubicin ,Anthracyclines ,Cumulative incidence ,ATRA ,Aged ,CONSOLIDATION ,Aged, 80 and over ,RISK ,Chemotherapy ,PETHEMA ,business.industry ,Remission Induction ,Cell Biology ,Hematology ,MOLECULAR REMISSION ,Middle Aged ,medicine.disease ,Survival Analysis ,Surgery ,Leukemia ,Patient Compliance ,Female ,business ,medicine.drug ,GIMEMA - Abstract
Therapeutic results in elderly patients with acute promyelocytic leukemia (APL) have been generally reported as less effective than for younger patients. Patients 60 years or older with APL who were enrolled in 2 successive multicenter PETHEMA studies received induction therapy with all-trans retinoic acid (ATRA) and idarubicin, consolidation with 3 anthracycline monochemotherapy courses with or without ATRA, and maintenance with ATRA and low-dose chemotherapy. Eighty-seven of 104 patients achieved complete remission (84%). Eighty-six proceeded to consolidation therapy (2 withdrew after the first and second courses). Deaths in remission occurred during consolidation and maintenance therapy in 3 and 4 patients, respectively. One patient showed molecular persistence after consolidation and 5 had a relapse. The 6-year cumulative incidence of relapse, leukemia-free survival, and disease-free survival were 8.5%, 91%, and 79%, respectively. A significantly higher incidence of low-risk patients found among the elderly, as compared to younger patients, may partially account for the low relapse rate observed. This study confirms the high antileukemic efficacy, low toxicity, and high degree of compliance of protocols using ATRA and anthracycline monochemotherapy for induction and consolidation therapy in elderly patients. (C) 2004 by The American Society of Hematology.
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- 2004
42. Immunophenotypic evaluation of the plasma cell compartment in multiple myeloma: a tool for comparing the efficacy of different treatment strategies and predicting outcome
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Joan Bladé, Luis Palomera, Julia Almeida, C. López-Berges, José L. Hernández, Maria Jesus Moro, Dolores Caballero, Joaquín Díaz-Mediavilla, Alberto Orfao, J. Fernández-Calvo, Jesús F. San Miguel, and Gema Mateo
- Subjects
Adult ,Male ,Oncology ,medicine.medical_specialty ,Pathology ,Neoplasm, Residual ,medicine.medical_treatment ,Plasma Cells ,Immunology ,Antineoplastic Agents ,Cell Count ,Plasma cell ,Transplantation, Autologous ,Biochemistry ,Disease-Free Survival ,Immunophenotyping ,Autologous stem-cell transplantation ,Internal medicine ,medicine ,Humans ,Multiple myeloma ,Aged ,Chemotherapy ,business.industry ,Hematopoietic Stem Cell Transplantation ,Cell Biology ,Hematology ,Middle Aged ,Prognosis ,medicine.disease ,Chemotherapy regimen ,humanities ,medicine.anatomical_structure ,Therapeutic Equivalency ,Female ,Stem cell ,Multiple Myeloma ,business ,Monoclonal gammopathy of undetermined significance - Abstract
Multiparametric immunophenotyping can be a sensitive method for analyzing the plasma cell (PC) compartment in patients with multiple myeloma because it discriminates between myelomatous and normal PCs. Using this approach, we compared the efficacy of high-dose chemotherapy followed by autologous stem cell transplantation (ASCT) with that of conventional chemotherapy. We found that ASCT provided a significantly greater reduction in the level of residual tumor PCs and with better recovery of normal PCs. This profile of coexistence of normal PCs and myelomatous PCs resembled that observed in monoclonal gammopathy of undetermined significance. We also found that treatment-induced changes in the PC compartment correlated with disease outcome. Thus, patients in whom at least 30% of gated PCs had a normal phenotype after treatment had a significantly longer progression-free survival (60 ± 6 months versus 34 ± 12 months;P = .02).
- Published
- 2002
43. Early immunophenotypical evaluation of minimal residual disease in acute myeloid leukemia identifies different patient risk groups and may contribute to postinduction treatment stratification
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C. López-Berges, Marcos González, Consuelo del Cañizo, Jesús F. San Miguel, Norma C. Gutiérrez, Joaquín Díaz-Mediavilla, José J. Pérez, Alberto Orfao, M J Calmuntia, María Belén Vidriales, and Fernando Ramos
- Subjects
Adult ,Male ,Oncology ,medicine.medical_specialty ,Neoplasm, Residual ,Immunology ,Biochemistry ,Immunophenotyping ,Bone Marrow ,Risk Factors ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Humans ,Risk factor ,Survival rate ,Acute leukemia ,business.industry ,Myeloid leukemia ,Cell Biology ,Hematology ,Flow Cytometry ,medicine.disease ,Minimal residual disease ,Survival Rate ,Leukemia ,Phenotype ,medicine.anatomical_structure ,Leukemia, Myeloid ,Acute Disease ,Female ,Bone marrow ,Neoplasm Recurrence, Local ,business - Abstract
Early response to therapy is one of the most important prognostic factors in acute leukemia. It is hypothesized that early immunophenotypical evaluation may help identify patients at high risk for relapse from those who may remain in complete remission (CR). Using multiparametric flow cytometry, the level of minimal residual disease (MRD) was evaluated in the first bone marrow (BM) in morphologic CR obtained after induction treatment from 126 patients with acute myeloid leukemia (AML) who displayed aberrant phenotypes at diagnosis. Based on MRD level, 4 different risk categories were identified: 8 patients were at very low risk (fewer than 10−4 cells), and none have relapsed thus far; 37 were at low risk (10−4 to 10−3 cells); and 64 were at intermediate risk (fewer than 10−3 to 10−2 cells), with 3-year cumulative relapse rates of 14% and 50%, respectively. The remaining 17 patients were in the high-risk group (more than 10−2 residual aberrant cells) and had a 3-year relapse rate of 84% (P = .0001). MRD level not only influences relapse-free survival but also overall survival (P = .003). The adverse prognostic impact was also observed when M3 and non-M3 patients with AML were separately analyzed, and was associated with adverse cytogenetic subtypes, 2 or more cycles to achieve CR, and high white blood cell counts. Multivariate analysis showed that MRD level was the most powerful independent prognostic factor, followed by cytogenetics and number of cycles to achieve CR. In conclusion, immunophenotypical investigation of MRD in the first BM in mCR obtained after AML induction therapy provides important information for risk assessment in patients with AML.
- Published
- 2001
44. Early Post Transplant Organ Toxicity after Allogeneic One-Haplotype Mismatch (Haplo) Hematopoietic Stem Cell Transplant (HSCT)
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Sabine Furst, Faezeh Legrand, Stefania Bramanti, Raynier Devillier, Catherine Faucher, Samia Harbi, Amir Meskine, Didier Blaise, Jerome Rey, Djamel Mokart, Angela Granata, Pierre Jean Weiller, Luca Castagna, Christian Chabannon, Clemence Mediavilla, and Diane Coso
- Subjects
medicine.medical_specialty ,education.field_of_study ,Acute leukemia ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,Immunology ,Population ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,medicine.disease ,Biochemistry ,Gastroenterology ,Surgery ,Transplantation ,Internal medicine ,medicine ,Mucositis ,Cumulative incidence ,business ,education ,Busulfan ,medicine.drug - Abstract
T-replete Haplo-HSCT is presently a major evolution in the field of allo-HSCT. Despite we miss prospective evaluation, we are faced to a high pace of diffusion worldwide. We however need to collect informations on many aspects. In this abstract we report an analysis of the early events and organ toxicities in the first patients transplanted in our program. Between 2011 and 2015, 117 patients treated for hematologic malignancy, receiving a Haplo-HSCT and post-transplant high dose cyclophosphamide (PT-HDCY) with a minimal follow-up of 100 days were analyzed. All organ toxicities were graded according to WHO scale and only grade 3-4 toxicities were analyzed: impact of age, Hematopoietic transplant comorbidity index (HCT-CI) and conditioning regimen were analyzed in this first step evaluation. Median age was 56 (19-73) and 58% were males (M/M: 35%; M/F: 21%; F/M: 23%; F/F: 21%; Donor origin: Sibling: 43%; offspring: 45%; Mother: 9%; Father: 3%). Patients were transplanted for: Acute Leukemia (AL): 32%; Non-AL myeloid malignancies: 19%; Lymphoma: 36%; Non-lymphoma Lymphoid malignancies: 13%. Patients were at high risk of both relapse (48% of active disease at the time of Haplo-HSCT, Disease risk index: low: 8%; Intermediate: 59%; high/very high: 33%) and non-relapse mortality [NRM] (65% had HCT-CI of 3 or more). Conditioning regimens were non-myeloablative TBI-based (NMAC), busulfan-based reduced intensity (RIC) and busulfan-based myeloablative conditioning (MAC) in 68%, 10% and 22%, respectively. PBSC were infused in 89% of the patients. Graft CD34/CD3 cells: 5.2 (0,8-14,8) / 261 (27-629) x 10e6/kg. Seventeen (15%) pts received a previous allo-HSCT and 7 (6%) pts with AL were included in a sequential debulking-transplant strategy. One-year overall survival, PFS and GRFS were 85%, 78% and 55%. Graft failure occurred in 3 pts (all with positive donor specific antibodies). Others reached ANC>0.5 G/L and platelet count>20G/L in a median time of 20 (14-38) and 31 (10-395) days post Haplo-HSCT. Day-100 cumulative incidence of grade 2-4 and 3-4 acute GVHD were 24% and 7%, respectively. Grade 3-4 WHO toxicities within 100 days: Pulmonary: 31%; Cardiac: 23%; Liver: 20%; Cystitis: 14%; mucositis: 12%; renal: 8%. 14 patients (12%) died within 100 days at a median of 40 (2-84) days (9 (9%) of the 100 patients with first and 5 (29%) of 17 patients with previous allo-HSCT). Causes of NRM were (n patients): infection: 6; Cardiac: 4; GVHD: 2; Neurologic: 2. In a multivariate analysis, grade 3-4 3-4 OMS cardiac toxicity occurred more frequently in pts older than 60 (HR: 0.42 (0.17-1.0), p=0.05, Figure 1A) and both oral mucositis (HR: 0.22 (0.06-0.72), p=0.01) and liver toxicity (HR: 0.32 (0.12-0.89), p=0.03) in patients treated with busulfan-based conditioning (Figure 1B). Seventy-nine (68%) patients experienced bacterial infection, 56 (48%) viral reactivation and 14 (12%) fungal infections. Pts older than 60 experienced more frequent bacterial infection than others (82% vs 58%: p=0.01, Figure 1A). In conclusion, this retrospective analysis suggests a higher incidence of peculiar organ toxicity as cardiac and bladder deserving further analysis. It also suggests that in a high-risk population (second allo-HSCT:15%; patients over 60 years: 38%; HCT-CI≥3: 65%; High/Very high DRI: 33%; transplanted with active disease: 48%early NRM is somehow limited allowing further development at the condition to take into account the main factors leading to these toxicities and to develop adapted care. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.
- Published
- 2016
45. A prospective PETHEMA study of tandem autologous transplantation versus autograft followed by reduced-intensity conditioning allogeneic transplantation in newly diagnosed multiple myeloma
- Author
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Joan Bladé, Angel Leon, Javier García-Frade, Juan José Lahuerta, Dolores Carrera, Jesús F. San Miguel, Pascual Fernandez Abellan, Laura Rosiñol, Felipe de Arriba, Miguel T. Hernandez, Joaquín Díaz-Mediavilla, Belen Hernandez, José D. González, José A. Pérez-Simón, Juan Bergua, Anna Sureda, and Javier de la Rubia
- Subjects
medicine.medical_specialty ,Transplantation Conditioning ,Allogeneic transplantation ,Immunology ,Graft vs Host Disease ,Kaplan-Meier Estimate ,Transplantation, Autologous ,Biochemistry ,Gastroenterology ,Disease-Free Survival ,Autologous stem-cell transplantation ,Internal medicine ,Immunopathology ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Transplantation, Homologous ,Autologous transplantation ,Prospective Studies ,Multiple myeloma ,Hematology ,business.industry ,Cell Biology ,Middle Aged ,medicine.disease ,Surgery ,Clinical trial ,Transplantation ,Multiple Myeloma ,business ,Stem Cell Transplantation - Abstract
One hundred ten patients with multiple myeloma (MM) failing to achieve at least near-complete remission (nCR) after a first autologous stem cell transplantation (ASCT) were scheduled to receive a second ASCT (85 patients) or a reduced-intensity-conditioning allograft (allo-RIC; 25 patients), depending on the human leukocyte antigen (HLA)–identical sibling donor availability. There was a higher increase in complete remission (CR) rate (40% vs 11%, P = .001) and a trend toward a longer progression-free survival (PFS; median, 31 months vs not reached, P = .08) in favor of allo-RIC. In contrast, it was associated with a trend toward a higher transplantation-related mortality (16% vs 5%, P = .07), a 66% chance of chronic graft-versus-host disease and no statistical difference in event-free survival and overall survival. Although the PFS plateau observed with allo-RIC is very encouraging, this procedure is associated with high morbidity and mortality, and therefore it should still be considered investigational and restricted to well-designed prospective clinical trials. This trial is registered at ClinicalTrials.gov ID number NCT00560053
- Published
- 2008
46. Persistent Benefit of VTD (Bortezomib/Thalidomide/Dexamethasone) As Pretransplant Induction Therapy for Multiple Myeloma: Long-Term Follow-up of a Randomized Phase 3 Pethema/GEM Study
- Author
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Rosiñol, Laura, primary, Oriol, Albert, additional, Teruel, Ana Isabel, additional, Hernandez, Dolores, additional, Blanchard, M Jesús, additional, De La Rubia, Javier, additional, Granell, Miquel, additional, Besalduch, Juan, additional, Palomera, Luis, additional, Gonzalez, Yolanda, additional, Echebeste, M Asunción, additional, Díaz-Mediavilla, Joaquín, additional, Hernandez, Miguel T, additional, de Arriba, Felipe, additional, Gutierrez, Norma Carmen, additional, Martín-Ramos, M Luisa, additional, Cibeira, M Teresa, additional, Mateos, Maria Victoria, additional, Martinez-Lopez, Joaquin, additional, Alegre, Adrian, additional, Lahuerta, Juan José, additional, San Miguel, Jesus F., additional, and Blade, Joan, additional
- Published
- 2014
- Full Text
- View/download PDF
47. Observational Prospective Registry for the Assessment of the Clinical Impact of Starting Anti-Myeloma Treatment at Biological Relapse
- Author
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Alegre, Adrian, primary, Gironella, Merche, additional, Bergua, Juan Miguel, additional, Gonzalez, Esther, additional, Escalante, Fernando, additional, Soler, Alfon, additional, Sampol, Antonia, additional, Gonzalez, Ana Pilar, additional, Cabañas, Valentin, additional, Lahuerta, Juan Jose, additional, Lopez, Aurelio, additional, Dios, Ana, additional, Bárez, Abelardo, additional, Ruiz, Antonio, additional, Vilanova, David, additional, and Díaz-Mediavilla, Joaquín, additional
- Published
- 2014
- Full Text
- View/download PDF
48. Tumor and Renal Response in Patients with Newly Diagnosed Multiple Mieloma and Renal Failure Treated with Bortezomib and Dexamethasone: Results of a Prospective Phase II Trial from Pethema/GEM
- Author
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Rosiñol, Laura, primary, Oriol, Albert, additional, Blanchard, M Jesús, additional, Palomera, Luis, additional, Mateos, Maria-Victoria, additional, De La Rubia, Javier, additional, Hernandez, Miguel T, additional, Díaz-Mediavilla, Joaquín, additional, Hernandez, Jose Mariano, additional, Jiménez, Raquel, additional, Motllo, Cristina, additional, Lahuerta, Juan José, additional, San Miguel, Jesus F., additional, and Blade, Joan, additional
- Published
- 2014
- Full Text
- View/download PDF
49. Kinetics of Response to Bortezomib/Thalidomide/Dexamethasone (VTD) in Multiple Myeloma: Implications for the Choice and Design of Pretransplantation Induction Regimens
- Author
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Rosiñol, Laura, primary, Oriol, Albert, additional, Teruel, Ana Isabel, additional, Hernández, Dolores, additional, Blanchard, M Jesús, additional, De La Rubia, Javier, additional, Granell, Miquel, additional, Besalduch, Juan, additional, Palomera, Luis, additional, Gonzalez, Yolanda, additional, Mª Asunción, Etxebeste, additional, Díaz-Mediavilla, Joaquín, additional, Hernandez, Miguel T., additional, de Arriba, Felipe, additional, Gutierrez, Norma Carmen, additional, Mateos, Maria-Victoria, additional, Martinez-Lopez, Joaquin, additional, Alegre, Adrian, additional, Feng, Huaibao, additional, van de Velde, Helgi, additional, Lahuerta, Juan José, additional, San Miguel, Jesus F., additional, and Blade, Joan, additional
- Published
- 2014
- Full Text
- View/download PDF
50. Molecular stratification model for prognosis in cytogenetically normal acute myeloid leukemia
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Teresa Bernal, José María Quiroga Alonso, Marcos González, María Jesús Peñarrubia, Carlos Santamaría, Jesús F. San Miguel, Alfonso García de Coca, Miguel Alcoceba, Cristina Fernández Pérez, Fernando Ramos, María C. Chillón, María Belén Vidriales, Joaquín Díaz-Mediavilla, Pilar Giraldo, Maria Dolores Caballero, Abelardo Bárez, Pascual Fernández-Abellán, Ramón García-Sanz, Ana Balanzategui, José Antonio Queizán, María Eugenia Sarasquete, and Juan N. Rodríguez
- Subjects
Oncology ,Adult ,Genetic Markers ,Male ,medicine.medical_specialty ,Pathology ,NPM1 ,Myeloid ,genetic structures ,Immunology ,Gene Expression ,Biochemistry ,Disease-Free Survival ,Transcriptional Regulator ERG ,Antigens, Neoplasm ,Risk Factors ,Internal medicine ,Proto-Oncogenes ,medicine ,Biomarkers, Tumor ,Humans ,Survival rate ,Aged ,PRAME ,Hematology ,Models, Genetic ,business.industry ,Cell Biology ,Middle Aged ,medicine.disease ,Prognosis ,MDS1 and EVI1 Complex Locus Protein ,DNA-Binding Proteins ,Survival Rate ,Leukemia ,Leukemia, Myeloid, Acute ,medicine.anatomical_structure ,Spain ,Cytogenetic Analysis ,Mutation ,Trans-Activators ,Female ,business ,Erg ,Nucleophosmin ,Transcription Factors - Abstract
We have evaluated 9 new molecular markers (ERG, EVI1, MLL-PTD, MN1, PRAME, RHAMM, and WT1 gene-expression levels plus FLT3 and NPM1 mutations) in 121 de novo cytogenetically normal acute myeloblastic leukemias. In the multivariate analysis, high ERG or EVI1 and low PRAME expressions were associated with a shorter relapse-free survival (RFS) and overall survival (OS). A 0 to 3 score was given by assigning a value of 0 to favorable parameters (low ERG, low EVI1, and high PRAME) and 1 to adverse parameters. This model distinguished 4 subsets of patients with different OS (2-year OS of 79%, 65%, 46%, and 27%; P = .001) and RFS (2-year RFS of 92%, 65%, 49%, and 43%; P = .005). Furthermore, this score identified patients with different OS (P = .001) and RFS (P = .013), even within the FLT3/NPM1 intermediate-risk/high-risk subgroups. Here we propose a new molecular score for cytogenetically normal acute myeloblastic leukemias, which could improve patient risk-stratification.
- Published
- 2009
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