1. Interleukin 2 gene transcription is regulated by Ikaros-induced changes in histone acetylation in anergic T cells
- Author
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Monika Paroder, Irene Puga, Sanmay Bandyopadhyay, Noemi Soto-Nieves, Myrianne Duré, and Fernando Macian
- Subjects
Interleukin 2 ,Transcription, Genetic ,T-Lymphocytes ,Immunology ,In Vitro Techniques ,Lymphocyte Activation ,Biochemistry ,Jurkat cells ,Epigenesis, Genetic ,Histones ,Ikaros Transcription Factor ,Jurkat Cells ,Mice ,medicine ,Animals ,Humans ,Promoter Regions, Genetic ,Interleukin 3 ,Immunobiology ,Zinc finger transcription factor ,Clonal Anergy ,Clonal anergy ,biology ,Models, Immunological ,Acetylation ,Cell Biology ,Hematology ,Th1 Cells ,Mice, Inbred C57BL ,Histone ,Interleukin 12 ,biology.protein ,Cancer research ,Interleukin-2 ,medicine.drug - Abstract
In T cells anergy may be evoked by an unbalanced stimulation of the T-cell receptor in the absence of costimulation. Anergic T cells are unresponsive to new antigen receptor engagement and do not produce interleukin 2. We present evidence that anergizing stimuli induce changes in histone acetylation, which mediates transcriptional repression of interleukin 2 expression. In response to calcium signaling, anergic T cells up-regulate the expression of Ikaros, a zinc finger transcription factor essential for lymphoid lineage determination. Ikaros binds to the interleukin 2 promoter where it induces histone deacetylation. Confirming the role of Ikaros in the induction of T-cell anergy, cells with reduced Ikaros activity show defective inactivation in response to an anergizing stimulus. We propose a model in which tolerizing stimuli induce epigenetic changes on the interleukin 2 locus that are responsible for the stable inhibition of the expression of this cytokine in anergic T cells.
- Published
- 2006