231 results on '"Gwynn OR"'
Search Results
2. Targeted deletion of α-adducin results in absent β- and γ-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice
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Robledo, Raymond F., Ciciotte, Steven L., Gwynn, Babette, Sahr, Kenneth E., Gilligan, Diana M., Mohandas, Narla, and Peters, Luanne L.
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- 2008
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3. Allogeneic Stem Cell Transplantation with Omidubicel: Long-Term Follow-up from a Single Center
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Cristina Gasparetto, Mitchell E. Horwitz, Anthony D. Sung, Stefanie Sarantopoulos, Aurelie Schwarzbach, Taewoong Choi, Richard D. Lopez, Laura Morrison, Edwin P. Alyea, Chenyu Lin, Einat Galamidi-Cohen, Nelson J. Chao, David A. Rizzieri, and Gwynn D. Long
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medicine.medical_specialty ,Long term follow up ,business.industry ,education ,Immunology ,Cell Biology ,Hematology ,Single Center ,Biochemistry ,Surgery ,Transplantation ,medicine ,Stem cell ,business ,health care economics and organizations - Abstract
Introduction Omidubicel is a hematopoietic stem cell graft derived from umbilical cord blood, composed of an ex vivo nicotinamide-expanded CD133+ stem cell fraction and a non-expanded CD133- T-cell-containing fraction. Omidubicel was the first ex vivo expanded stem cell graft to demonstrate durable engraftment and the first to be transplanted as a single, stand-alone graft following myeloablative conditioning. Recent prospective clinical trials have established the short-term safety and efficacy of hematopoietic cell transplantation (HCT) with omidubicel, both as a stand-alone graft and in conjunction with an unmanipulated cord blood unit (Horwitz et al, 2014, 2019, and 2021). This retrospective study reports on the long-term experience with omidubicel HCT in patients with advanced hematologic malignancies at a single institution. Methods All patients with hematologic malignancies who had undergone HCT and engrafted with omidubicel between November 2010 and January 2020 were eligible for analysis. Patients who experienced primary graft failure or engrafted with an unmanipulated unit were excluded. A retrospective review was performed under the auspices of an IRB approved protocol to determine their long-term outcomes. The primary endpoints were survival and long-term hematopoiesis. Secondary endpoints included immune reconstitution, disease relapse, chronic graft versus host disease (GVHD), and secondary hematologic malignancies. R 4.1.0 was used to perform Kaplan Meier survival analysis and competing risk analyses of cumulative incidence via Gray's method. Results A total of 26 patients received an omidubicel graft during the study period, of whom 22 were eligible for analysis. Three patients engrafted with an unmanipulated cord blood graft and 1 patient experienced primary graft failure. The median age of the eligible patients was 48 yrs (range, 18 - 62 yrs) and 8 patients (36%) were non-white. The most common disease types were AML (36%), MDS (32%), and ALL (18%). By disease risk index, 5 patients (23%) were considered high risk, 13 (59%) were intermediate risk, 3 (14%) were low risk, and 1 (5%) was unevaluable. At the time of data cutoff, the median follow-up was 2.3 yrs (range, 0.1 - 10 yrs) for all eligible patients and 7.4 yrs (range, 1.8 - 10 yrs) for the 11 survivors. At last follow-up, 11 patients had passed away due to disease relapse (64%), acute GVHD (27%), or infection (9%). The estimated 10-year overall survival and disease-free survival were 48.5% (95% CI, 31.0% - 75.7%) (Fig 1A) and 43.6% (95% CI, 26.8% - 70.9%), respectively. Durable omidubicel-derived trilineage hematopoiesis was observed at up to ten years of follow-up (Fig 2A-2C). Serial quantitative assessments of lymphocyte subsets suggest stability of the median CD3+, CD4+, and CD8+ T cell, as well as CD19+ B cell and NK cell counts. These levels were within normal ranges starting at 6 months post-transplant and up until 8 years of follow-up. Using competing risk analysis, the 10-year estimated cumulative incidence of relapse was 31.8% (95% CI, 13.6% - 51.8%). The 10-year estimated cumulative incidence of chronic GVHD was 31.8% (95% CI, 13.4% - 52.1%). Of the 7 patients who experienced chronic GVHD, only 2 still required systemic immunosuppression at last follow-up. One patient experienced secondary graft failure requiring a rescue haploidentical transplant on day 202. There were no cases of secondary hematologic malignancies. Conclusions This study suggests that omidubicel provides long-term sustainable hematopoiesis and immune competence at follow-up periods of up to 10 years. This is the first characterization of the long-term safety of an ex vivo expanded hematopoietic stem cell graft. While there has been concern that ex vivo expansion approaches may compromise the integrity of long-term repopulating hematopoietic stem cells, there was only one documented case of secondary graft failure in this cohort. Furthermore, there was no evidence for the development of clonal hematopoiesis, however molecular testing was not performed to confirm this observation. Chronic GVHD was observed, but most patients were able to wean off immunosuppression. In summary, omidubicel is a safe and reliable stem cell source for patients in need of hematopoietic cell transplantation, particularly for underrepresented racial and ethnic minorities. Figure 1 Figure 1. Disclosures Choi: Janssen Biotech: Speakers Bureau. Gasparetto: Janssen: Consultancy; Sanofi: Consultancy, Honoraria, Speakers Bureau; Oncopeptide: Consultancy, Honoraria, Speakers Bureau; Gsk: Consultancy, Honoraria, Speakers Bureau; Karyopharm: Consultancy, Honoraria, Speakers Bureau. Lopez: PhosphoGam: Current holder of individual stocks in a privately-held company, Current holder of stock options in a privately-held company. Rizzieri: AbbVie: Membership on an entity's Board of Directors or advisory committees; AROG: Membership on an entity's Board of Directors or advisory committees; Bayer: Membership on an entity's Board of Directors or advisory committees; Sanofi: Membership on an entity's Board of Directors or advisory committees; Teva: Membership on an entity's Board of Directors or advisory committees; Acrotech: Membership on an entity's Board of Directors or advisory committees; Seattle Genetics: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Stemline: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Incyte: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Kite: Honoraria, Speakers Bureau; Karyopharm: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Celgene: Membership on an entity's Board of Directors or advisory committees; Celltrion: Membership on an entity's Board of Directors or advisory committees; Gilead: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Mustang: Membership on an entity's Board of Directors or advisory committees; Novartis: Membership on an entity's Board of Directors or advisory committees; Jazz: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Pfizer: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Amgen: Honoraria; Pharmacyclics: Honoraria. Sarantopoulos: Rigel: Other: Advisory Board. Sung: Merck: Research Funding; Novartis: Research Funding; Enterome: Research Funding; Seres: Research Funding; AVROBIO: Consultancy; Abbott Nutrition: Honoraria; Clasado: Other: Research Product; DSM: Other: Research Product. Galamidi-Cohen: Gamida Cell, Ltd: Current Employment. Schwarzbach: Gamida Cell: Current Employment. Horwitz: Gamida Cell: Research Funding.
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- 2021
4. Female Gender Is Associated with Improved Long-Term Survival Following Allogeneic Hematopoietic Stem Cell Transplant
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Islam, Prioty, primary, Jin, Haesu, additional, Cao, Felicia, additional, Bohannon, Lauren M., additional, Ren, Yi, additional, Chao, Nelson J., additional, Choi, Taewoong, additional, Gasparetto, Cristina, additional, Horwitz, Mitchell E., additional, Long, Gwynn D., additional, Lopez, Richard D., additional, Rizzieri, David A, additional, Sarantopoulos, Stefanie, additional, and Sung, Anthony D., additional
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- 2020
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5. Morphologic Leukemia-Free State in Acute Myeloid Leukemia Is Sufficient for Successful Allogeneic Hematopoietic Stem Cell Transplant
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Pabon, Cindy M., primary, Li, Zhiguo, additional, Hennig, Therese, additional, De Castro, Carlos, additional, Neff, Jadee, additional, Horwitz, Mitchell E., additional, Leblanc, Thomas W., additional, Long, Gwynn D., additional, Lopez, Richard D., additional, Sung, Anthony D., additional, Chao, Nelson J., additional, Gasparetto, Cristina, additional, Sarantopoulos, Stefanie, additional, Adams, Donna B., additional, Erba, Harry P., additional, and Rizzieri, David A, additional
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- 2020
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6. Morphologic Leukemia-Free State in Acute Myeloid Leukemia Is Sufficient for Successful Allogeneic Hematopoietic Stem Cell Transplant
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Thomas W. LeBlanc, Therese Hennig, Carlos M. de Castro, Stefanie Sarantopoulos, Anthony D. Sung, Richard D. Lopez, Cristina Gasparetto, Zhiguo Li, Cindy M. Pabon, Mitchell E. Horwitz, Jadee L. Neff, Harry P. Erba, Nelson J. Chao, Gwynn D. Long, David A. Rizzieri, and Donna Adams
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Adult ,Male ,medicine.medical_specialty ,education ,Immunology ,Population ,Kaplan-Meier Estimate ,Biochemistry ,Young Adult ,Correspondence ,Humans ,Transplantation, Homologous ,Medicine ,In patient ,MORPHOLOGIC LEUKEMIA-FREE STATE ,RC254-282 ,health care economics and organizations ,Aged ,education.field_of_study ,Cancer stem cells ,business.industry ,Hematopoietic Stem Cell Transplantation ,Complete remission ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Cell Biology ,Hematology ,Middle Aged ,Transplantation ,Leukemia, Myeloid, Acute ,Treatment Outcome ,Oncology ,Family medicine ,Honorarium ,Female ,Allogeneic hematopoietic stem cell transplant ,business ,Bristol-Myers ,High risk disease - Abstract
Allogeneic hematopoietic cell transplant (HCT) improves survival in patients with relapsed or high risk acute myeloid leukemia (AML). Complete remission (CR) is typically a pre-requisite for transplantation, though many do not achieve a formal CR. The traditional AML treatment starts with induction chemotherapy, followed by assessment of response to guide next steps. Response criteria definitions differ between that of the National Comprehensive Cancer Network (NCCN), utilized by the majority of clinicians, and the Center for International Blood and Marrow Research (CIBMTR) data registry utilized by transplant centers, making interpretation of the impact of HCT difficult. Definitions for morphologic complete remission (CR) are the same, however complete remission with incomplete hematologic recovery (CRi) differs and the CIBMTR does not recognize the morphologic leukemia-free state (MLFS), thus mis-identifying such patients and preventing clear treatment guidelines for this population. We conducted a retrospective study, identifying a cohort of 35 AML patients at our center who underwent allogeneic HCT while in MLFS, to evaluate characteristics in patient demographics, disease status, treatment(s), and outcomes. From our cohort, the median overall survival (OS) was 14 months, however 37% were alive and in remission with median follow-up of survivors of five years. Twenty three percent had progression of disease following transplant. Non-relapse mortality (NRM) was 35% with leading cause of death being infection. Our study reveals that transplant can induce long-term survival in patients with acute leukemia who are in MLFS at the start of induction, similar to data for patients with high risk disease in early relapse or in later remissions. Early transplantation while in MLFS and not waiting for full count recovery may protect patients from toxicities of further chemotherapeutic agents or prevent unnecessary delays that may allow for infections or other barriers to arise, and requires further study. Disclosures Leblanc: American Cancer Society, BMS, Duke University, NINR/NIH, Jazz Pharmaceuticals, Seattle Genetics: Research Funding; UpToDate: Patents & Royalties: Royalties; Agios, AbbVie, and Bristol Myers Squibb/Celgene: Speakers Bureau; AstraZeneca: Research Funding; AbbVie, Agios, Amgen, AstraZeneca, CareVive, BMS/Celgene, Daiichi-Sankyo, Flatiron, Helsinn, Heron, Otsuka, Medtronic, Pfizer, Seattle Genetics, Welvie: Consultancy, Honoraria, Membership on an entity's Board of Directors or advisory committees. Rizzieri:Karyopharm: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Kite: Honoraria, Speakers Bureau; Incyte: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Stemline: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Sanofi: Membership on an entity's Board of Directors or advisory committees; Novartis: Membership on an entity's Board of Directors or advisory committees; Mustang: Membership on an entity's Board of Directors or advisory committees; Celltrion: Membership on an entity's Board of Directors or advisory committees; Celgene: Membership on an entity's Board of Directors or advisory committees; Gilead: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Jazz: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Pfizer: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Seattle Genetics: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; AROG: Membership on an entity's Board of Directors or advisory committees; abbvie: Membership on an entity's Board of Directors or advisory committees; Teva: Membership on an entity's Board of Directors or advisory committees; Acrobiotech: Membership on an entity's Board of Directors or advisory committees; Bayer: Membership on an entity's Board of Directors or advisory committees.
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- 2020
7. Female Gender Is Associated with Improved Long-Term Survival Following Allogeneic Hematopoietic Stem Cell Transplant
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Yi Ren, Prioty Islam, Taewoong Choi, Felicia Cao, Cristina Gasparetto, Lauren Bohannon, Haesu Jin, Mitchell E. Horwitz, Stefanie Sarantopoulos, Anthony D. Sung, Richard D. Lopez, Gwynn D. Long, Nelson J. Chao, and David A. Rizzieri
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Oncology ,medicine.medical_specialty ,business.industry ,education ,Immunology ,Cell Biology ,Hematology ,Biochemistry ,Internal medicine ,Long term survival ,medicine ,Allogeneic hematopoietic stem cell transplant ,business ,health care economics and organizations - Abstract
Introduction: Life expectancy for long-term survivors of allogeneic hematopoietic stem cell transplant (allo-HCT) is significantly lower compared to that of the age-matched general population, despite a relatively low primary disease relapse rate >2 years post-transplant. Long-term transplant-related complications instead account for most mortality in this patient population. These include chronic graft-versus-host disease (cGVHD), infection, organ failure, and secondary cancers. In addition, gender is emerging as a critical determinant of outcome in the immediate post-transplant setting. However, less is known regarding gender effects on outcomes of long-term survivors. We retrospectively investigated the impact of recipient gender and donor-recipient gender mismatch on outcomes of long-term survivors of allo-HCT. Methods: We performed a retrospective analysis using Duke University's Adult Blood and Marrow Transplant database, supplemented by individual patient chart review. Inclusion criteria consisted of long-term survivors of first allo-HCT, excluding syngeneic, between 1995 - 2015 for a hematologic malignancy. A long-term survivor is defined as having been alive with documented follow-up to at least five years following allo-HCT. Patient characteristics were summarized as count (%) for categorical variables and median (interquartile range) for continuous variables. Fisher's exact tests or t-tests were used to compare difference between groups. Overall survival was estimated using the Kaplan-Meier method and multivariable Cox proportional hazard model. Patients who had received cord blood allograft were excluded from donor-recipient gender survival analyses. SAS version 9.4 (SAS Institute, Cary, NC) and R 3.5.0 were used to perform statistical analyses. Results: Over this 20-year period, 1103 patients underwent allo-HCT, with 247 (22%) meeting inclusion criteria. Of these 247, males and females had similar demographic and treatment characteristics (Table 1). However, significantly more deaths after the 5-year landmark occurred in male recipients (Figure 1, p value=0.003). To estimate whether this was due to the general population-wide shorter life expectancy for males, we performed Kaplan-Meier estimates of survival for patients aged < 50 years at transplant and aged ≥ 50 years at transplant, with similar results for both groups (Figure 2, p value=0.006). Interestingly, donor gender did not have a significant impact on overall survival in multivariate analysis (Table 2), and differences in overall survival of donor-recipient gender pairs was driven by patient gender (Figure 3, p value =0.007). A separate multivariate analysis of interaction between donor-recipient gender pairs further demonstrated that significant differences in overall survival were due to patient gender (Table 3). In addition to patient gender, only cGVHD retained significance as a covariate with impact on overall survival in multivariate analysis (Table 2, Table 3). Men experienced slightly higher rates and increased severity of cGVHD, and a greater percentage of cGVHD-related mortality as compared to females, though these findings were not statistically significant (Table 1). Conclusion: In this long-term survival analysis of allo-HCT adult patients, one of the only to include follow-up to 15 years, our results show that women survive significantly longer than men irrespective of their age at transplant. This outcome is independent of other common pre-transplant prognostic indicators such as donor gender or performance status at transplant. Inferior survival for males is consistent with survival outcomes described in another large and fully risk annotated HCT cohort, and in solid organ transplants such as lung and kidney. Gathering evidence suggests a biologic basis for long-term gender-determined outcomes, possibly due to differing rates or severity of cGVHD or sustained alloimmune tolerance in females. Male patients should be counseled on their possible increased long-term risk. Prospective and larger studies are warranted to validate these retrospective clinical results. Disclosures Rizzieri: Gilead: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Jazz: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Pfizer: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Seattle Genetics: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Stemline: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Incyte: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; Kite: Honoraria, Speakers Bureau; Karyopharm: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; abbvie: Membership on an entity's Board of Directors or advisory committees; AROG: Membership on an entity's Board of Directors or advisory committees; Bayer: Membership on an entity's Board of Directors or advisory committees; Celgene: Membership on an entity's Board of Directors or advisory committees; Celltrion: Membership on an entity's Board of Directors or advisory committees; Mustang: Membership on an entity's Board of Directors or advisory committees; Novartis: Membership on an entity's Board of Directors or advisory committees; Sanofi: Membership on an entity's Board of Directors or advisory committees; Teva: Membership on an entity's Board of Directors or advisory committees; Acrobiotech: Membership on an entity's Board of Directors or advisory committees.
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- 2020
8. Daily Chlorhexidine Gluconate Bathing Reduces the Rate of Bloodstream Infections in Adults Undergoing Inpatient Hematopoietic Stem Cell Transplantation
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Giri, Vinay K., primary, Kegerreis, Kristin G., additional, Ren, Yi, additional, Bohannon, Lauren M., additional, Lobaugh-Jin, Erica, additional, Messina, Julia A., additional, Matthews, Anita, additional, Allen, Deborah H., additional, Mowery, Yvonne M., additional, Saullo, Jennifer L., additional, Jung, Sin-Ho, additional, Choi, Taewoong, additional, Gasparetto, Cristina, additional, Horwitz, Mitchell E., additional, Long, Gwynn D., additional, Lopez, Richard D., additional, Rizzieri, David A., additional, Sarantopoulos, Stefanie, additional, Chao, Nelson J., additional, and Sung, Anthony D., additional
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- 2019
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9. Geriatric Assessment Identifies Impairments in Younger Candidates for Allogeneic Hematopoietic Stem Cell Transplantation
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Lew, Meagan V., primary, Ren, Yi, additional, Lowder, Yen P., additional, Romero, Kristi M., additional, Thompson, Jillian C., additional, Bohannon, Lauren M., additional, Cohen, Harvey, additional, Bartlett, David A., additional, Pastva, Amy M., additional, Morey, Miriam, additional, Hall, Katherine, additional, Smith, Patrick, additional, Peters, Katherine B., additional, Somers, Tamara, additional, Kelleher, Sarah, additional, Smith, Sophia, additional, Wischmeyer, Paul, additional, Lin, Pao-Hwa, additional, Thorpe, Glynnis, additional, Minor, Kerry, additional, Adler, Allison, additional, Wiggins, Kristi, additional, Hennig, Therese, additional, Helms, Tanya, additional, Welch, Renee, additional, Hicks, Whitney, additional, Eren, Margaret, additional, Porter, Rebecca, additional, Matthews, Brittany, additional, Liu, JoAnn, additional, Burleson, Jill, additional, Andrew, Whitney, additional, Aberant, Thomas, additional, Engemann, Ashley K., additional, Henshall, Bethany, additional, Darby, Maurisa, additional, Valea, Renea, additional, Proch, Christina, additional, Dellascio, Michelle, additional, Pittman, Alyssa, additional, Choi, Taewoong, additional, Gasparetto, Cristina, additional, Long, Gwynn D., additional, Lopez, Richard D., additional, Rizzieri, David A., additional, Sarantopoulos, Stefanie, additional, Horwitz, Mitchell E., additional, Chao, Nelson J., additional, and Sung, Anthony D., additional
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- 2019
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10. Decreased Mortality after the First Year of Allogeneic Hematopoietic Stem Cell Transplant in Recipients of Umbilical Cord Blood Vs. Matched Related or Matched Unrelated Donors
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Bohannon, Lauren M., primary, Page, Kristin M., additional, Ren, Yi, additional, Jung, Sin-Ho, additional, Giri, Vinay K., additional, Lew, Meagan V., additional, Kelly, Matthew, additional, Choi, Taewoong, additional, Gasparetto, Cristina, additional, Long, Gwynn D., additional, Lopez, Richard D., additional, Rizzieri, David A., additional, Sarantopoulos, Stefanie, additional, Chao, Nelson J., additional, Horwitz, Mitchell E., additional, and Sung, Anthony D., additional
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- 2019
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11. Evaluation of the Oral SYK Inhibitor Fostamatinib in Patients after Allogeneic Transplantation for Chronic Graft-Versus-Host Disease
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McManigle, William C., primary, DiCioccio, Rachel A., additional, Anand, Sarah, additional, Li, Zhiguo, additional, Poe, Jonathan C, additional, Nichols, Krista R., additional, Suthers, Amy N., additional, Jia, Wei, additional, Corbet, Kelly, additional, Covington, Megan, additional, Lloyd-Cowden, Julia, additional, Lopez, Richard D., additional, Long, Gwynn D., additional, Horwitz, Mitchell E., additional, Gasparetto, Cristina, additional, Sung, Anthony D., additional, Chao, Nelson J., additional, Rizzieri, David A., additional, and Sarantopoulos, Stefanie, additional
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- 2019
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12. Pre-Transplant Hepatic Steatosis (fatty liver) Predicts Chronic Graft-Vs-Host Disease but Does Not Affect Mortality
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Maung, Ko Ko Ko, primary, Chaudhry, Mohammad, additional, Ren, Yi, additional, Jung, Sin-Ho, additional, Romero, Kristi M., additional, Corbet, Kelly, additional, Chao, Nelson J., additional, Choi, Taewoong, additional, Diehl, Anna Mae, additional, Diehl, Louis F., additional, Gasparetto, Cristina, additional, Horwitz, Mitchell E., additional, Long, Gwynn D., additional, Lopez, Richard D., additional, Rizzieri, David A., additional, Sarantopoulos, Stefanie, additional, Sullivan, Keith, additional, Bashir, Mustafa R., additional, and Sung, Anthony D., additional
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- 2019
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13. Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex
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Gwynn, Babette, Martina, Jose A., Bonifacino, Juan S., Sviderskaya, Elena V., Lamoreux, M. Lynn, Bennett, Dorothy C., Moriyama, Kengo, Huizing, Marjan, Helip-Wooley, Amanda, Gahl, William A., Webb, Lisa S., Lambert, Amy J., and Peters, Luanne L.
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- 2004
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14. Identification of quantitative trait loci that modify the severity of hereditary spherocytosis inwan, a new mouse model of band-3 deficiency
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Peters, Luanne L., Swearingen, Rebecca A., Andersen, Sabra G., Gwynn, Babette, Lambert, Amy J., Li, Renhua, Lux, Samuel E., and Churchill, Gary A.
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- 2004
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15. Decreased Mortality after the First Year of Allogeneic Hematopoietic Stem Cell Transplant in Recipients of Umbilical Cord Blood Vs. Matched Related or Matched Unrelated Donors
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Matthew S. Kelly, Stefanie Sarantopoulos, Richard D. Lopez, Anthony D. Sung, Gwynn D. Long, Vinay K. Giri, Sin-Ho Jung, Taewoong Choi, Lauren Bohannon, Meagan V. Lew, Nelson J. Chao, Mitchell E. Horwitz, Cristina Gasparetto, Kristin Page, David A. Rizzieri, and Yi Ren
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medicine.medical_specialty ,Univariate analysis ,Multivariate analysis ,business.industry ,Proportional hazards model ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,Lower risk ,medicine.disease ,Biochemistry ,Transplantation ,Graft-versus-host disease ,Internal medicine ,Statistical significance ,Medicine ,business - Abstract
Introduction: Allogeneic hematopoietic stem cell transplantation (allo-HCT) provides a potential cure for a multitude of diseases but can result in high rates of morbidity and mortality. These outcomes have been associated with donor choice, with umbilical cord blood (UCB) perceived as an inferior option to matched related or matched unrelated donors (MRD/MUD) due to increased morbidity and mortality the first year after allo-HCT due to delayed engraftment and increased graft versus host disease (GvHD). At the same time, studies have shown that recipients of cells from younger donors do better, and UCB is the youngest donor cell source available. Given that UCB may have long-term benefits, we evaluated long-term survival in recipients of UCB vs. MRD/MUD in patients who had survived at least 1 year. Methods: This is a retrospective analysis of ≥1 year (≥365 days) survivors of first allo-HCT from the Duke Adult Bone Marrow Transplant (ABMT) program over a twenty year period from January 1, 1996 to December 31, 2015. Detailed clinical data was extracted from the Duke ABMT database and electronic medical record. Patients who either died or were lost to follow-up within 1 year of transplant were excluded. Also excluded were those who received an allo-HCT for a disease other than a hematologic malignancy and those who received haploidentical or mismatched adult cells, as these donor sources have been associated with worse outcomes and the goal was to compare UCB to MRD/MUD. Patient characteristics included gender, race, ethnicity, transplant diagnosis, transplant year, hematopoietic cell transplantation-specific comorbidity index (HCT-CI), Karnofsky performance score (KPS) at transplant workup, age at transplant, donor cell characteristics, conditioning regimen, acute GvHD (aGvHD), date of relapse, and date of death. Chi-square tests or Fisher's exact tests were used to compare categorical variables, as appropriate, and Wilcoxon Rank Sum tests or t-tests were used to compare continuous variables, as appropriate. A Kaplan-Meier estimator was used to analyze overall survival. A Cox Proportional Hazard model with stepwise selection with significance of entry=0.1 and significance of stay=0.2 was used to evaluate the patient characteristics of age, gender, race, disease, KPS, conditioning type, history of aGvHD, and decade of transplant and adjusted for selected covariates to compare the two groups. Results: Over the 20-year study period, 848 patients received a first allo-HCT, of whom 456 (54%) survived at least 1 year post-HCT: 102 (22%) UCB and 354 (78%) MRD/MUD. UCB recipients were more likely to be younger (median 42 years vs. 50 years, p= Discussion: Among patients who survived at least 1 year post-HCT, those who received UCB had a significantly lower risk of mortality on univariate analysis than those who received adult cells. Though this finding was no longer statistically significant on multivariate analysis, there was a strong trend and the lack of statistical significance could be an effect of the small sample size. Additional research across larger populations (e.g. CIBMTR) is warranted to further explore the clinical characteristics and long-term health outcomes between the two groups, especially given the potential for newer UCB approaches such as ex-vivo expansion to decrease the early post-HCT morbidity and mortality associated with UCB and delayed engraftment. If other studies support improved long-term outcomes with UCB, those findings coupled with advances in UCB transplants may promote UCB as a donor source. Disclosures Gasparetto: Janssen: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; BMS: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Celgene: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed . Rizzieri:Celgene, Gilead, Seattle Genetics, Stemline: Other: Speaker; AbbVie, Agios, AROG, Bayer, Celgene, Gilead, Jazz, Novartis, Pfizer, Sanofi, Seattle Genetics, Stemline, Teva: Other: Advisory Board; AROG, Bayer, Celgene, Celltron, Mustang, Pfizer, Seattle Genetics, Stemline: Consultancy; Stemline: Research Funding. Horwitz:Abbvie Inc: Membership on an entity's Board of Directors or advisory committees. Sung:Novartis: Research Funding; Merck: Research Funding; Seres: Research Funding.
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- 2019
16. Evaluation of the Oral SYK Inhibitor Fostamatinib in Patients after Allogeneic Transplantation for Chronic Graft-Versus-Host Disease
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William C McManigle, Gwynn D. Long, Sarah Anand, David A. Rizzieri, Nelson J. Chao, Julia M. Lloyd-Cowden, Krista Rowe Nichols, Kelly Corbet, Zhiguo Li, Wei Jia, Mitchell E. Horwitz, Cristina Gasparetto, Jonathan C. Poe, Rachel A. DiCioccio, Stefanie Sarantopoulos, Amy N. Suthers, Richard D. Lopez, Megan Covington, and Anthony D. Sung
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Immunology ,Cell Biology ,Hematology ,Fostamatinib ,medicine.disease ,Biochemistry ,Donor lymphocyte infusion ,law.invention ,Transplantation ,Graft-versus-host disease ,Tolerability ,Randomized controlled trial ,immune system diseases ,law ,hemic and lymphatic diseases ,Internal medicine ,medicine ,business ,Liver function tests ,Adverse effect ,medicine.drug - Abstract
Our group previously showed that B cells signal aberrantly through the B cell receptor (BCR) in allogeneic hematopoietic stem cell transplant (HCT) patients with active chronic graft-versus-host disease (cGVHD). Preclinical mouse studies have demonstrated the importance of the proximal BCR molecule, spleen tyrosine kinase (SYK), in cGVHD development. Hypothesizing that the oral small molecule SYK inhibitor, fostamatinib, would safely target aberrant BCR-activated B cells in HCT patients, we are conducting a single-center, investigator-initiated phase 1 trial (NCT02611063). Our primary objective is to evaluate the safety and tolerability of fostamatinib in patients early after HCT and in those with refractory active cGVHD. Secondary objectives include assessment of cGVHD manifestations, B cell activation, and immune recovery. Methods: All patients receive HCT treatment per program standards at Duke University. Prophylaxis (P-cGVHD) subjects enroll 80-150 days after HCT and have no evidence of cGVHD. P-cGVHD subjects receive drug for up to 1 year post-transplant (215-285 fostamatinib days). Steroid-refractory cGVHD (SR-cGVHD) subjects enroll with active cGVHD that persists despite systemic high-dose steroids. SR-cGVHD subjects receive drug for up to 365 days total. For all enrollees, modified continual reassessment criteria are used to determine starting dose (100mg daily, 150mg daily, or 100mg twice BID) and any needed dose modifications. We monitor for drug-limiting toxicities (DLTs), adverse events (AEs), and cGVHD manifestations using NIH cGVHD consensus criteria at up to 12 follow-up visits. Results: 15 of a planned 18 total patients have enrolled. In the P-cGVHD group (n=5), of the 4 patients who completed treatment (mean 239 fostamatinib days), 1 patient developed cGVHD while enrolled and 2 patients subsequently developed cGVHD, 4 and 6 weeks after study completion. The fifth P-cGVHD subject discontinued therapy on study day 155 (provider decision to initiate donor lymphocyte infusion for low CD3+ chimerism). In the SR-cGVHD group (n=10), 2 patients completed treatment (mean 365 fostamatinib days); 3 patients withdrew (mean 132 fostamatinib days), for non-cardiac chest pain, progression of cGVHD, and moved away; and 5 patients are actively enrolled (mean 207 fostamatinib days). Both SR-cGVHD patients who completed the study clinically improved while on fostamatinib and requested continuation of drug. A total of 2, 9, and 4 patients have been initiated on 100mg daily, 150mg daily, and 100mg BID, respectively. At the 100mg daily dose, no DLTs were noted. At the 150mg daily dose, 1 patient developed liver function test (LFT) elevation. At the 100mg BID dose, 2 patients developed LFT elevation and 1 patient developed non-cardiac chest pain. One patient required dose adjustment: 100mg BID to 150mg daily, for LFT elevation. Two serious AEs possibly related to fostamatinib occurred: 1 patient developed non-cardiac chest pain and 1 patient developed a deep venous thrombosis. No probably- or definitely-related serious AEs occurred. To assess whether fostamatinib effectively targets aberrantly activated B cells, we examined subjects' whole blood using flow cytometry. When comparing CD19+ B cells on study day 1 versus study day 60 in the SR-cGVHD group (n=7), we found the relative proportion of CD19+CD38+IgDlow plasmablast-like cells was decreased (p=0.03, Fig 1A-B), suggesting fostamatinib 'hit target.' Importantly, in the P-cGVHD group, total lymphocyte and B cell counts did not decrease during day 1 to day 225 (Fig 1C-D), suggesting fostamatinib did not affect immune recovery when given early after HCT. Further investigations with functional assays are underway. Conclusions: This study demonstrates for the first time that fostamatinib is safe and tolerated in HCT recipients both early after transplant and in those with active cGVHD. Importantly, fostamatinib does not appear to hinder lymphocyte or B cell recovery when initiated between days 80-150 after HCT. Additionally, fostamatinib may effectively target aberrantly activated B cells in patients with active SR-cGVHD. Fostamatinib, now FDA-approved for treatment of immune thrombocytopenia, merits a phase 2, randomized controlled trial to assess efficacy as a prophylactic agent against cGVHD. This work was supported by a National Institutes of Health grant, NIH (NHLBI) R01 HL 129061. Fostamatinib was supplied by Rigel. Disclosures Horwitz: Abbvie Inc: Membership on an entity's Board of Directors or advisory committees. Gasparetto:BMS: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Celgene: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Janssen: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed . Sung:Novartis: Research Funding; Merck: Research Funding; Seres: Research Funding. Rizzieri:Celgene, Gilead, Seattle Genetics, Stemline: Other: Speaker; AbbVie, Agios, AROG, Bayer, Celgene, Gilead, Jazz, Novartis, Pfizer, Sanofi, Seattle Genetics, Stemline, Teva: Other: Advisory Board; AROG, Bayer, Celgene, Celltron, Mustang, Pfizer, Seattle Genetics, Stemline: Consultancy; Stemline: Research Funding.
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- 2019
17. Geriatric Assessment Identifies Impairments in Younger Candidates for Allogeneic Hematopoietic Stem Cell Transplantation
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Cristina Gasparetto, Meagan V. Lew, Katherine S. Hall, Pao-Hwa Lin, Tanya Helms, Margaret Eren, Whitney Andrew, Renee Welch, Glynnis Thorpe, Stefanie Sarantopoulos, Ashley K. Engemann, Patrick Smith, Sophia K. Smith, Michelle Dellascio, Yen P. Lowder, Richard D. Lopez, Jillian C. Thompson, Anthony D. Sung, Thomas Aberant, Taewoong Choi, Sarah A. Kelleher, David A. Rizzieri, Harvey J. Cohen, Maurisa Darby, Brittany Matthews, Renea Valea, JoAnn Liu, Alyssa Pittman, Whitney Hicks, Tamara J. Somers, Therese Hennig, Kristi Romero, Yi Ren, David A. Bartlett, Amy M. Pastva, Bethany Henshall, Katherine B. Peters, Paul E. Wischmeyer, Rebecca Porter, Kristi Wiggins, Nelson J. Chao, Allison Adler, Miriam C. Morey, Mitchell E. Horwitz, Christina Proch, Lauren Bohannon, Kerry King Minor, Jill Burleson, and Gwynn D. Long
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Oncology ,medicine.medical_specialty ,Functional impairment ,business.industry ,medicine.medical_treatment ,Immunology ,Geriatric assessment ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,Physical function ,Biochemistry ,Chemotherapy regimen ,Transplantation ,Internal medicine ,Medicine ,Patient evaluation ,Allogeneic hematopoietic stem cell transplant ,business - Abstract
Introduction: Geriatric assessment (GA) is a multidimensional evaluation of patient health and function that may detect impairments not identified as part of routine care, predict treatment-related morbidity and mortality, and inform treatment plans. Given evidence of these benefits, the National Comprehensive Cancer Network Clinical Practice Guidelines in Oncology (NCCN Guidelines®) recommend GA for older candidates of hematopoietic stem cell transplantation (HCT). However, both older and younger HCT candidates will often receive multiple rounds of chemotherapy before HCT, leading to functional impairments in all age groups. Furthermore, HCT patients often experience a significant gap between when they are first evaluated and actually proceed to transplant (e.g., while a donor search is conducted), creating an opportunity to identify impairments and optimize function prior to transplant. Methods: To address this opportunity, we created a clinical pre-HCT optimization program (C-POP) to evaluate physical function, cognitive function, nutritional status, and mental health in all adults who were deemed potential candidates for allogeneic HCT by a HCT physician. We applied this standard of care program to all adult candidates for HCT, regardless of age, with the goal of identifying functional impairments and then referring patients to services to optimize those impairments prior to HCT. We defined impairments using validated measures and compared results to established norms or scoring, controlling for age and gender where appropriate (e.g., the cut-off for six-minute walk distance was adjusted for age, gender, height, and weight, while the cut-off for falls was any fall regardless of characteristics). Patients with impairments were referred to the appropriate supportive care (e.g., physical function impairment -> referral to physical therapy). Results were prospectively analyzed at new patient evaluation (NPE), which was the first time the patient met a HCT physician and sign-off, which occurred within a week before starting transplant. While the program is ongoing, we present here the results of patients evaluated between October 16th, 2017 and July 1st, 2019. Patients are divided into three pre-specified age groups: =60 years old, with results compared using a chi-squared test. Results: We evaluated 115 patients: 21 (18%) =60 years). There were no differences between the age groups in other demographics (gender, race, and ethnicity). At NPE, 93 (81%) met criteria for at least 1 impairment in physical function, cognitive function, nutritional status, or mental health; 62 (54%) met criteria for impairments in 2 or more areas. Surprisingly, patients =60 years (36/54, 67%) (p=0.03). Of those 115 patients, 52 (45%) proceeded to HCT, including 12 (57%) =60 years (p=0.75); of those patients who have not proceeded to HCT, 40 (35%) will never proceed to HCT (e.g., deemed not a candidate after functional evaluation or died of disease prior to HCT) while 23 (20%) are still awaiting HCT (e.g., donor search ongoing). Patients who proceeded to HCT were less likely to have mental health impairments (2/52, 4% vs. 9/40, 23%, p=0.006). Of the 52 who were seen at new patient evaluation and proceeded to transplant, 40 (77%) were seen at sign off. Of those who had impairments at NPE, 12/23 (52%) improved their physical function to normal limits, 4/9 (44%) improved their cognitive function, and 9/13 (69%) improved their nutritional status by the time of sign-off (of those who were seen at sign-off, none had mental health impairments at NPE). Discussion: These results demonstrate that younger as well as older candidates for HCT exhibit a high degree of functional impairment. However, this impairment could be amenable to improvement prior to HCT. These findings support application of GA to all HCT candidates regardless of age. We will investigate the effect of referred interventions (e.g., physical therapy, seeing a dietician) in improving functional impairments in future studies, as well as look at the effect of these findings on HCT outcomes. Disclosures Wiggins: Incyte, Inc.: Speakers Bureau. Gasparetto:Janssen: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Celgene: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; BMS: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed . Rizzieri:Millennium: Speakers Bureau; Novartis: Consultancy; AbbVie: Consultancy; Pfizer: Consultancy; Amgen: Consultancy; Incyte: Consultancy, Speakers Bureau; TEVA: Consultancy; Spectrum: Consultancy; Kite Pharma: Consultancy; Gilead Sciences: Consultancy, Speakers Bureau; Seattle Genetics: Consultancy, Speakers Bureau; Jazz Pharmaceuticals: Speakers Bureau. Horwitz:Abbvie Inc: Membership on an entity's Board of Directors or advisory committees. Sung:Novartis: Research Funding; Merck: Research Funding; Seres: Research Funding.
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- 2019
18. Daily Chlorhexidine Gluconate Bathing Reduces the Rate of Bloodstream Infections in Adults Undergoing Inpatient Hematopoietic Stem Cell Transplantation
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Yvonne M. Mowery, Lauren Bohannon, David A. Rizzieri, Kristin G. Kegerreis, Deborah H. Allen, Julia A. Messina, Anita Matthews, Gwynn D. Long, Sin-Ho Jung, Nelson J. Chao, Yi Ren, Taewoong Choi, Cristina Gasparetto, Erica Lobaugh-Jin, Vinay K. Giri, Stefanie Sarantopoulos, Richard D. Lopez, Anthony D. Sung, Jennifer L. Saullo, and Mitchell E. Horwitz
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medicine.medical_specialty ,business.industry ,Incidence (epidemiology) ,Immunology ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Intensive care unit ,law.invention ,Transplantation ,Regimen ,Levofloxacin ,law ,Internal medicine ,medicine ,Infection control ,Antibiotic prophylaxis ,business ,Febrile neutropenia ,medicine.drug - Abstract
Introduction: While hematopoietic stem cell transplantation (HSCT) has great therapeutic potential, intensive conditioning regimens and variability in time to stem cell engraftment result in a period of pancytopenia and immunosuppression in which patients are vulnerable to infection. Bloodstream infections (BSIs) occur in 20-45% of inpatient autologous and allogeneic transplant patients, leading to prolonged hospitalization and increased mortality. One method of infection prevention is daily application of the antiseptic chlorhexidine gluconate (CHG). Daily CHG bathing, either with a CHG wash or with application of CHG-impregnated cloths, has been shown to reduce the incidence of all-cause hospital-acquired BSIs in critically ill patients, such as those in the ICU, though very few studies include HSCT patients. Methods: We conducted an observational cohort study to assess the impact of daily CHG bathing on the rate of BSIs among adults undergoing inpatient HSCT at the Duke University Medical Center. Patients were included if they were admitted for pre-transplant conditioning and inpatient monitoring for allogeneic or autologous HSCT from January 2016 through December 2018. CHG bathing was instituted in January 2017 for all patients admitted to the inpatient HSCT unit using 2% CHG-impregnated cloths (SAGE™), providing one year of data with no CHG bathing and two years of data with bathing. Patients were tracked from admission until unit discharge, transfer, or first infection. Laboratory-confirmed bloodstream infections (LCBI), central-line associated bloodstream infections (CLABSI), and mucosal barrier injury laboratory-confirmed bloodstream infections (MBI-LCBI) were determined per CDC/NHSN definitions. An additional variable of "clinically-significant infection" was recorded; this included both laboratory-confirmed BSIs and infections deemed significant by the treatment team but that did not meet CDC/NHSN criteria. For example, patients that were febrile, hypotensive, and treated with antibiotics, but with only one positive culture of a common commensal were deemed to have a clinically-significant BSI. Because not all patients adhered to CHG, patients were grouped into four categories by rate of daily CHG usage: High (>75%), Medium (50-75%), Low (1-49%), and None (0%). Baseline characteristics and clinical outcomes between groups were compared via ANOVA, Chi-squared test, or Cochran-Armitage two-sided trend test. Multivariate analysis using the Fine-Grey subdistribution hazard model was conducted to compare time to all infection variables, accounting for the effects of CHG usage, antibiotic prophylaxis regimen, and type of transplant. Results: We evaluated 192 patients hospitalized for HSCT, including 118 (62%) allogeneic transplants and 74 (38%) autologous transplants. Of these, 25 (13%) had high CHG usage, 33 (17%) medium, 45 (23%) low, and 89 (46%) none. Demographics and transplant characteristics were evenly matched between the CHG usage groups with the exception that high usage groups were more likely to receive levofloxacin for antibiotic prophylaxis (p=0.003). Increased CHG usage was significantly associated with decreased incidence of clinically-significant infection (p=0.006), CLABSI (p=0.04), and MBI-LCBI (p=0.002) (Table 1). Multivariate analysis did not demonstrate a significant contribution of antibiotic prophylaxis regimen. No significant difference was found between CHG usage groups in median days to stem cell engraftment, incidence of febrile neutropenia or C. difficile infection, or rashes requiring medical treatment. There were no rashes attributable to CHG usage. Among patients who were VRE rectal swab negative on admission, there was a significant trend toward lower rates of VRE acquisition with increasing CHG usage (High CHG 13.6% vs No CHG 25.3%, p=0.02). Discussion: Increased CHG usage is associated with a significant trend toward lower rates of clinically-significant infection, CLABSI, MBI-LCBI, and VRE colonization in adult inpatients undergoing HSCT. Effects are most strongly seen at >75% daily CHG usage. There were no adverse effects due to CHG application. The significant decrease in MBI-LCBI with topical CHG suggests an interaction between the skin microbial environment and enteric organisms. Therefore, further research exploring the effects of CHG on the skin and gut microbiota is warranted. Disclosures Gasparetto: BMS: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Janssen: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Celgene: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed . Horwitz:Abbvie Inc: Membership on an entity's Board of Directors or advisory committees. Rizzieri:Celgene, Gilead, Seattle Genetics, Stemline: Other: Speaker; AbbVie, Agios, AROG, Bayer, Celgene, Gilead, Jazz, Novartis, Pfizer, Sanofi, Seattle Genetics, Stemline, Teva: Other: Advisory Board; AROG, Bayer, Celgene, Celltron, Mustang, Pfizer, Seattle Genetics, Stemline: Consultancy; Stemline: Research Funding. Sung:Novartis: Research Funding; Merck: Research Funding; Seres: Research Funding.
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- 2019
19. Pre-Transplant Hepatic Steatosis (fatty liver) Predicts Chronic Graft-Vs-Host Disease but Does Not Affect Mortality
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Cristina Gasparetto, Louis F. Diehl, Keith M. Sullivan, Nelson J. Chao, Anna Mae Diehl, Mustafa R. Bashir, Kristi Romero, Taewoong Choi, Gwynn D. Long, Anthony D. Sung, Sin-Ho Jung, Mitchell E. Horwitz, Stefanie Sarantopoulos, Richard D. Lopez, Yi Ren, Ko K. Maung, Kelly Corbet, David A. Rizzieri, and Mohammad Chaudhry
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medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Immunology ,Fatty liver ,Retrospective cohort study ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Transplantation ,Graft-versus-host disease ,Liver biopsy ,Internal medicine ,medicine ,Steatohepatitis ,Steatosis ,Liver function tests ,business - Abstract
Introduction: Allogeneic hematopoietic stem cell transplant (allo-HCT) remains the only curative treatment for many hematologic conditions. However, significant mortality risk and risk of severe graft-vs-host disease (GvHD) limit its usefulness. Some patient characteristics such as performance status are used routinely as prognostic markers for poor outcomes after transplant. Steatohepatitis is a metabolic dysfunction manifesting as increased hepatic adiposity and resulting inflammation. A pre-clinical murine study suggests steatohepatitis is associated with GvHD. We assessed whether pre-HCT hepatic steatosis was associated with risk of developing GvHD and post transplant mortality. Methods: This retrospective study reviewed adult patients who underwent allogeneic stem cell transplants at Duke University over a 10-year period between 2008 and 2017 and had a computed tomography scan (CT) of the abdomen and pelvis with contrast within a 1-year period preceding the transplant. Patients with an acute illness as the indication for CT were excluded. We measured the attenuation of the liver vs. spleen as an estimate of hepatic steatosis. We used the widely accepted definition of hepatic steatosis as present when the difference in average attenuation between the spleen and liver was greater than or equal to 10 Hounsfield units and as absent when the difference was less than 10 Hounsfield units. Baseline pre-HCT factors as well as post-HCT outcomes were abstracted from the medical record. Results: We identified 80 patients with pre-HCT CT scans; of those, 59 (74%) had hepatic steatosis as defined above. Patients with hepatic steatosis tended to be older (median 52 vs. 38 years, p=0.0522); there were no statistically significant differences in gender, race, ethnicity, height, weight, Karnofsky Performance Status, HCT Comorbidity Index, history of diabetes, history of coronary artery disease, history of elevated liver function tests, primary disease, conditioning regimen, or stem cell source. Hepatic steatosis did not predict acute GvHD grade II-IV (OR 1.003, 95% CI 0.259 - 3.888, p=0.997). However, it was significantly associated with chronic GvHD on both univariate as well as multivariate analysis (Table 1, multivariate OR 3.83, 95% CI 1.11 - 13.28, p=0.023). There was no difference in overall survival or non-relapse mortality between the two groups. Conclusions: This study suggests that hepatic steatosis may predict chronic GvHD but does not predict acute GvHD or overall/non-relapse mortality. This study can be improved by using other imaging techniques such as a noncontrast CT, which is more accurate for assessment of hepatic steatosis. It is also important to note that imaging can only be used to predict the existence of hepatic steatosis; lobular inflammation, which is required to diagnose steatohepatitis, can only be proven on biopsy. Larger studies with liver biopsy or other noninvasive imaging measures of liver stiffness and steatosis will be needed to confirm the above results. Disclosures Gasparetto: Celgene: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; Janssen: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed ; BMS: Consultancy, Honoraria, Other: Travel, accommodations, or other expenses paid or reimbursed . Horwitz:Abbvie Inc: Membership on an entity's Board of Directors or advisory committees. Rizzieri:Gilead Sciences: Consultancy, Speakers Bureau; Pfizer: Consultancy; Novartis: Consultancy; Millennium: Speakers Bureau; Jazz Pharmaceuticals: Speakers Bureau; AbbVie: Consultancy; Spectrum: Consultancy; Kite Pharma: Consultancy; Incyte: Consultancy, Speakers Bureau; TEVA: Consultancy; Seattle Genetics: Consultancy, Speakers Bureau; Amgen: Consultancy. Bashir:Metacrine, Inc.: Research Funding; ProSciento, Inc.: Research Funding; Pinnacle Clinical Research: Research Funding; CymaBay Therapeutics: Research Funding; Siemens Healthcare: Research Funding; NGM Biopharmaceuticals: Research Funding; Madrigal Pharmaceulticals: Research Funding. Sung:Novartis: Research Funding; Merck: Research Funding; Seres: Research Funding.
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- 2019
20. Successful allogeneic engraftment of mismatched unrelated cord blood following a nonmyeloablative preparative regimen
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Rizzieri, David A., Long, Gwynn D., Vredenburgh, James J., Gasparetto, Christina, Morris, Ashley, Stenzel, Timothy T., Davis, Patti, and Chao, Nelson J.
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- 2001
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21. Treatment of steroid-refractory acute graft-versus-host disease with anti-CD147 monoclonal antibody ABX-CBL
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Deeg, H. Joachim, Blazar, Bruce R., Bolwell, Brian J., Long, Gwynn D., Schuening, Friedrich, Cunningham, John, Rifkin, Robert M., Abhyankar, Sunil, Briggs, Adrienne D., Burt, Richard, Lipani, John, Roskos, Lorin K., White, J. Michael, Havrilla, Nancy, Schwab, Gisela, and Heslop, Helen E.
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- 2001
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22. Defects in the cappuccino (cno) gene on mouse chromosome 5 and human 4p cause Hermansky-Pudlak syndrome by an AP-3–independent mechanism
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Gwynn, Babette, Ciciotte, Steven L., Hunter, Susan J., Washburn, Linda L., Smith, Richard S., Andersen, Sabra G., Swank, Richard T., Dell'Angelica, Esteban C., Bonifacino, Juan S., Eicher, Eva M., and Peters, Luanne L.
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- 2000
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23. Hematopoietic Stem Cell Transplantation at Home
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Sung, Anthony D., primary, Nichols, Krista R., additional, Messina, Julia A., additional, Romero, Kristi, additional, Dalton, Tara, additional, Bush, Amy, additional, Covington, Megan, additional, Shelby, Rebecca, additional, Frith, Jennifer, additional, Gasparetto, Cristina J., additional, Horwitz, Mitchell E., additional, Long, Gwynn D., additional, Lopez, Richard, additional, Rizzieri, David A., additional, Sarantopoulos, Stefanie, additional, Sullivan, Keith M., additional, and Chao, Nelson J., additional
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- 2017
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24. A Randomized Phase 3 Study of Peripheral Blood Progenitor Cell Mobilization With Stem Cell Factor and Filgrastim in High-Risk Breast Cancer Patients
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Shpall, Elizabeth J., Wheeler, Catherine A., Turner, Stewart A., Yanovich, Saul, Brown, Randy A., Pecora, Andrew L., Shea, Thomas C., Mangan, Kenneth F., Williams, Stephanie F., LeMaistre, C. Fred, Long, Gwynn D., Jones, Roy, Davis, Mark W., Murphy-Filkins, Robyn, Parker, William R.L., and Glaspy, John A.
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- 1999
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25. Hematopoietic Stem Cell Transplantation at Home
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Megan Covington, Stefanie Sarantopoulos, Julia A. Messina, Amy Bush, Richard D. Lopez, Gwynn D. Long, Tara Dalton, Anthony D. Sung, Cristina Gasparetto, Jennifer Frith, Krista Rowe Nichols, David A. Rizzieri, Mitchell E. Horwitz, Rebecca A. Shelby, Nelson J. Chao, Keith M. Sullivan, and Kristi Romero
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medicine.medical_specialty ,Blood transfusion ,Performance status ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Transplantation ,Clinical research ,Quality of life ,Emergency medicine ,Cohort ,medicine ,House call ,business ,Febrile neutropenia - Abstract
Introduction: Patients receiving autologous (auto) and allogeneic (allo) hematopoietic stem cell transplantation (HCT) typically undergo extensive inpatient hospitalizations, daily "day hospital" visits, or a combination of the two. This aspect of transplant poses a variety of challenges, ranging from nosocomial infections to hospital-induced delirium to lifestyle adjustments. By keeping patients at home, we may lower complications and improve quality of life, as well as potentially lower costs and resource utilization. Methods: We conducted a phase 1 study of home HCT to investigate the safety and feasibility of implementing this strategy in the United States. Patients interested in participating needed to live within a 30 minute drive of a transplant center and underwent a home inspection to assess suitability and safety (e.g. absence of black mold). Eligible patients would still receive pre-transplant conditioning at the hospital or day hospital, but they would be discharged home after receiving their stem cell infusion. The goal was to keep trial participants at home for the duration of transplant. In the morning, nurse practitioners or physician assistants made daily house calls to conduct assessments, examine patients, and draw blood for laboratory studies. These studies were run at the hospital, and in the afternoon a nurse returned to the patient's home to provide home blood transfusions, home intravenous fluids, electrolytes, or antibiotics, or other interventions. If an acute event occurred that could not be safely managed at home (e.g. first evaluation of febrile neutropenia), patients returned to the hospital or day hospital for further workup and care. Likewise, patients returned to the hospital or day hospital for certain routine procedures, such as intravenous methotrexate given as part of graft-versus-host disease prophylaxis or first blood transfusion to ensure there were no reactions. Transplant outcomes were monitored by the medical team throughout the patient's care and confirmed by a clinical research nurse or specialist by chart review. Stool samples, to assess changes in the gut microbiome, were collected at baseline and weekly for the first four weeks, at day 60, 100, and day 180. Samples will undergo 16s rRNA sequencing to identify the taxonomic groups of bacteria present in the gut. Results: Twenty-two patients received home HCT (Table 1). This included 6 allos and 16 autos. Ages ranged from 34-63 years for allos and 46-74 years for autos. In both groups, 2/3 or more of patients had a baseline Karnofsky Performance Status of 70 or 80. Patients in the allo group spent 72% of their days entirely at home, while patients in the auto group spent 52% of their days at home. The main reason for returning to the hospital or day hospital were febrile neutropenia (4 allos and 9 autos). Aside from CMV reactivation (3 of 6 allos, 50%), only 2 allos (33%) and 2 autos (13%) developed bloodstream infections. Three allos developed GVHD; interestingly, these were the three patients that spent the most time in the hospital/day hospital even prior to Day 30 (median 23 days vs. 11 days), even prior to development of GVHD. There was one case of treatment-related mortality (GVHD) in the entire cohort. Overall, patients and their caregivers endorsed the program, providing numerous expressions of appreciation and gratitude on exit interviews, and quality of life was well-preserved as assessed by FACT-BMT: median scores for autos stayed the same when comparing baseline (median 113) to day 30 (median 114) and increased on day 100 (median 124). Discussion: Our results suggest that home HCT is safe and feasible. Despite including mostly older adults with suboptimal performance status, patients did quite well at home. They were able to maintain their quality of life and had low rates of infectious complications. Though patients did have to return to the hospital or day hospital at various times during transplant, keeping patients out of the hospital for even half the duration of transplant could have tremendous cost savings that would offset the increased staffing and travel required for house calls. Studies of the gut microbiome are pending to test the hypothesis that home HCT will preserve the gut microbiome, thereby preventing GVHD. In addition, a randomized phase 2 study of home vs. standard transplant for allogeneic HCT is currently in progress. Disclosures Sung: Novartis: Research Funding; Merck: Research Funding; Cellective: Research Funding. Gasparetto: Janssen, BMS, Celgene: Consultancy; Janssen, BMS, Celgene: Other: Travel, accommodations, or other expenses paid or reimbursed; Celgene: Research Funding; Janssen, BMS, Celgene, Takeda: Honoraria. Rizzieri: Shire: Research Funding; Erytech: Research Funding.
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- 2017
26. Adult Umbilical Cord Blood Transplantation Using Myeloablative Total Body Irradiation, Fludarabine, and Thiotepa Conditioning
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Anand, Sarah, primary, Thomas, Samantha, additional, Corbet, Kelly, additional, Gasparetto, Cristina, additional, Lopez, Richard, additional, Long, Gwynn D., additional, Morris, Ashley K., additional, Rizzieri, David A., additional, Sarantopoulos, Stefanie, additional, Sullivan, Keith M, additional, Sung, Anthony D., additional, Chao, Nelson J., additional, and Horwitz, Mitchell E., additional
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- 2016
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27. Identification of quantitative trait loci that modify the severity of hereditary spherocytosis in wan, a new mouse model of band-3 deficiency
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Renhua Li, Gary A. Churchill, Luanne L. Peters, Babette Gwynn, Samuel E. Lux, Amy J. Lambert, Rebecca A. Swearingen, and Sabra G. Andersen
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Erythrocyte Indices ,Candidate gene ,Quantitative Trait Loci ,Immunology ,Mean corpuscular hemoglobin ,Spherocytosis, Hereditary ,Hematocrit ,Quantitative trait locus ,Biochemistry ,Hereditary spherocytosis ,Mice ,Anion Exchange Protein 1, Erythrocyte ,medicine ,Animals ,Humans ,Band 3 ,Mean corpuscular volume ,Crosses, Genetic ,Genetics ,Mice, Inbred C3H ,Base Sequence ,biology ,medicine.diagnostic_test ,Membrane Proteins ,Spectrin ,Blood Proteins ,DNA ,Cell Biology ,Hematology ,medicine.disease ,Mice, Mutant Strains ,Cytoskeletal Proteins ,Disease Models, Animal ,Red blood cell ,Phenotype ,medicine.anatomical_structure ,Codon, Terminator ,biology.protein ,Lod Score - Abstract
Defects in red blood cell (RBC) membrane skeleton components cause hereditary spherocytosis (HS). Clinically, HS varies significantly even among individuals with identical gene defects, illustrating the profound effects of genetic background on disease severity. We exploited a new spontaneous mouse model, wan, which arose on the inbred C3H/HeJ strain, to identify quantitative trait loci (QTL) that modify the HS phenotype. Homozygous wan mice have severe HS due to a complete deficiency of erythroid band 3. A QTL analysis of RBC count, hemoglobin, hematocrit, mean corpuscular volume (MCV), and mean corpuscular hemoglobin content (MCHC) was performed in wan/wan mice from an F2 intercross between C3H/HeJ+/wan and CAST/Ei+/+ F1 hybrids. Hematologic and survival data from C3H, CAST/Ei F2 wan homozygotes support the hypothesis that genetic modifiers significantly influence the band-3 null HS phenotype. Significant QTL were identified for the MCV trait only, suggesting that RBC membrane characteristics are a target for modifier gene action. The most significant quantitative trait locus, Hsm1 (hereditary spherocytosis modifier 1), localizes to mouse Chromosome 12 and is dominant. The peak LOD score was obtained with a marker for Spnb1 encoding erythroid β-spectrin, an obvious candidate gene. (Blood. 2004;103: 3233-3240)
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- 2004
28. Adult Umbilical Cord Blood Transplantation Using Myeloablative Total Body Irradiation, Fludarabine, and Thiotepa Conditioning
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Mitchell E. Horwitz, Kelly Corbet, Samantha M. Thomas, Sarah Anand, Nelson J. Chao, Gwynn D. Long, Keith M. Sullivan, David A. Rizzieri, Stefanie Sarantopoulos, Richard D. Lopez, Cristina Gasparetto, Ashley Morris, and Anthony D. Sung
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medicine.medical_specialty ,Neutrophil Engraftment ,Platelet Engraftment ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,ThioTEPA ,Hematopoietic stem cell transplantation ,Total body irradiation ,Biochemistry ,Gastroenterology ,Surgery ,Fludarabine ,Transplantation ,Internal medicine ,medicine ,Cumulative incidence ,business ,medicine.drug - Abstract
Introduction: Umbilical cord blood (UCB) extends the curative potential of stem cell transplantation to adult patients without a suitable donor. The most commonly used myeloablative preparative regimen results in an unacceptably high 6-month treatment related mortality rate of approximately 32% (Barker J et al. Br J Haematol. 2015). In an attempt to reduce treatment related mortality, we piloted a modified myeloablative regimen with total body irradiation (TBI), fludarabine, and thiotepa. We report clinical outcomes from a cohort of patients who received single or double UCBT after conditioning with this regimen. Methods: Thirty-one consecutive adult patients ≥ 18 years old with hematologic malignancies who underwent single or double umbilical cord blood transplantation at Duke University from 2010 to 2015 were included in this study. The conditioning regimen consisted of thiotepa 5 mg/kg/day i.v. x 2 days (days -11 to -10), TBI 150 cGy twice a day for total nine fractions (1350 cGy days -9 to -5), and fludarabine 40 mg/m2/day i.v. x 4 days (days -5 to -2). Cord blood units were matched to the recipient at 4 or more HLA loci (intermediate-resolution for A and B, high-resolution for DRB1). Graft versus host disease (GVHD) prophylaxis was with tacrolimus (target level 10-15 ng/ml) and mycophenolate mofetil 1000 mg TID. Antimicrobial prophylaxis and supportive care measures including GCSF administration until ANC > 1000 were conducted per institutional protocol. Probabilities of neutrophil and platelet recovery, acute and chronic GVHD, and treatment-related mortality were estimated by the cumulative incidence method. Relapse-free and overall survival rates were estimated by the Kaplan-Meier method. Results: Thirty-one patients (median age 46 years; range, 19-65) with hematologic malignancies were evaluated. Twenty-four patients (77%) had acute leukemia or myelodysplastic syndrome, while 7 patients (23%) had non-Hodgkin's lymphoma or multiple myeloma. By the "Disease Risk Index" (Armand P et al. Blood. 2014), 20 patients (65%) had low or intermediate risk disease, while 11 patients (35%) had high or very high risk disease. 30 patients underwent double UCB and 1 patient received single UCB transplantation. The median cryopreserved total nucleated cell dose was 5.4 x 107/kg (range: 3.2-8.4 x 107/kg). The cumulative incidence of neutrophil engraftment was 90% (95% CI, 82%-99%; Figure 1) at a median time of 21 days (95% CI, 19-26). Three patients did not have neutrophil engraftment; two patients had early death at days 7 and 14 prior to engraftment, while one patient had graft failure requiring second transplant. The cumulative incidence of platelet engraftment was 86% (95% CI, 75%-97%) at a median time of 47 days (95% CI, 37-73). Cumulative incidences of grades II-IV and grades III-IV acute GVHD were 48% (95% CI, 34%-69%) and 10% (95% CI, 3%-28%), respectively. The overall incidence of chronic GVHD was 40% (95% CI, 27%-59%), with 17% (95% CI, 8%-37%) of patients experiencing moderate to severe chronic GVHD. Treatment-related mortality at 6 months was 13% (Figure 2), while at 1 year and 3 years was 27% and 33%, respectively. With a median follow-up of 35.5 months (95% CI, 12.7-52.2), disease-free and overall survival at 3 years was 51% (95% CI, 29%-69%) and 57% (95% CI, 36%-73%), respectively. Conclusion: UCB transplantation with the modified myeloablative conditioning regimen of TBI, fludarabine, and thiotepa results in reliable neutrophil engraftment with reduced early treatment related mortality as compared to standard myeloablative conditioning consisting of TBI, fludarabine, and cyclophosphamide. It provides a promising disease-free and overall survival in an older (median age 46), heterogeneous patient population. This regimen represents a reasonable alternative to standard conditioning with TBI, fludarabine, and cyclophosphamide and warrants further study. Figure 1 Figure 1. Figure 2 Figure 2. Disclosures No relevant conflicts of interest to declare.
- Published
- 2016
29. Defects in the cappuccino (cno) gene on mouse chromosome 5 and human 4p cause Hermansky-Pudlak syndrome by an AP-3–independent mechanism
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Linda L. Washburn, Richard T. Swank, Luanne L. Peters, Juan S. Bonifacino, Richard S. Smith, Susan J. Hunter, Esteban C. Dell'Angelica, Steven L. Ciciotte, Sabra G. Andersen, Babette Gwynn, and Eva M. Eicher
- Subjects
Mutation ,Immunology ,Cell Biology ,Hematology ,Biology ,medicine.disease ,medicine.disease_cause ,Biochemistry ,Oculocutaneous albinism ,Lipofuscin ,Cell biology ,medicine.anatomical_structure ,Lysosome ,medicine ,Albinism ,Hermanski-Pudlak Syndrome ,Hermansky–Pudlak syndrome ,Melanosome - Abstract
Defects in a triad of organelles (melanosomes, platelet granules, and lysosomes) result in albinism, prolonged bleeding, and lysosome abnormalities in Hermansky-Pudlak syndrome (HPS). Defects in HPS1, a protein of unknown function, and in components of the AP-3 complex cause some, but not all, cases of HPS in humans. There have been 15 inherited models of HPS described in the mouse, underscoring its marked genetic heterogeneity. Here we characterize a new spontaneous mutation in the mouse, cappuccino (cno), that maps to mouse chromosome 5 in a region conserved with human 4p15-p16. Melanosomes ofcno/cno mice are immature and dramatically decreased in number in the eye and skin, resulting in severe oculocutaneous albinism. Platelet dense body contents (adenosine triphosphate, serotonin) are markedly deficient, leading to defective aggregation and prolonged bleeding. Lysosomal enzyme concentrations are significantly elevated in the kidney and liver. Genetic, immunofluorescence microscopy, and lysosomal protein trafficking studies indicate that the AP-3 complex is intact in cno/cno mice. It was concluded that the cappuccino gene encodes a product involved in an AP-3–independent mechanism critical to the biogenesis of lysosome-related organelles.
- Published
- 2000
30. Defects in the cappuccino (cno) gene on mouse chromosome 5 and human 4p cause Hermansky-Pudlak syndrome by an AP-3–independent mechanism
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Babette Gwynn, Steven L. Ciciotte, Susan J. Hunter, Linda L. Washburn, Richard S. Smith, Sabra G. Andersen, Richard T. Swank, Esteban C. Dell'Angelica, Juan S. Bonifacino, Eva M. Eicher, and Luanne L. Peters
- Subjects
Immunology ,Cell Biology ,Hematology ,Biochemistry - Abstract
Defects in a triad of organelles (melanosomes, platelet granules, and lysosomes) result in albinism, prolonged bleeding, and lysosome abnormalities in Hermansky-Pudlak syndrome (HPS). Defects in HPS1, a protein of unknown function, and in components of the AP-3 complex cause some, but not all, cases of HPS in humans. There have been 15 inherited models of HPS described in the mouse, underscoring its marked genetic heterogeneity. Here we characterize a new spontaneous mutation in the mouse, cappuccino (cno), that maps to mouse chromosome 5 in a region conserved with human 4p15-p16. Melanosomes ofcno/cno mice are immature and dramatically decreased in number in the eye and skin, resulting in severe oculocutaneous albinism. Platelet dense body contents (adenosine triphosphate, serotonin) are markedly deficient, leading to defective aggregation and prolonged bleeding. Lysosomal enzyme concentrations are significantly elevated in the kidney and liver. Genetic, immunofluorescence microscopy, and lysosomal protein trafficking studies indicate that the AP-3 complex is intact in cno/cno mice. It was concluded that the cappuccino gene encodes a product involved in an AP-3–independent mechanism critical to the biogenesis of lysosome-related organelles.
- Published
- 2000
31. A Phase I-II Study of the Combination of Bendamustine and Pomalidomide with Dexamethasone in Patients with Relapsed or Refractory Multiple Myeloma.
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Gasparetto, Cristina, primary, Green, Michael, additional, Srinivasan, Anandgopal, additional, Kang, Yubin, additional, Rizzieri, David A., additional, Decastro, Carlos, additional, Diehl, Louis F., additional, Beaven, Anne, additional, Li, Zighuo, additional, Rao, Arati V., additional, Garrett, Anderson, additional, Tuchman, Sascha, additional, and Long, Gwynn D., additional
- Published
- 2015
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32. High-Dose Therapy and Autologous Hematopoietic Progenitor Cell Transplantation for Recurrent or Refractory Hodgkin's Disease: Analysis of the Stanford University Results and Prognostic Indices
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Robert S. Negrin, Ruby M. Wong, Karl G. Blume, Nelson J. Chao, Richard T. Hoppe, Wendy W. Hu, Gwynn D. Long, Byron W. Brown, and Sandra J. Horning
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Chemotherapy ,medicine.medical_specialty ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,Total body irradiation ,Biochemistry ,Chemotherapy regimen ,Surgery ,Transplantation ,Regimen ,Medicine ,business ,Survival analysis ,medicine.drug - Abstract
One hundred nineteen patients with relapsed or refractory Hodgkin's disease (HD) received high-dose therapy followed by autologous hematopoietic progenitor cell transplantation. Three preparatory regimens, selected on the basis of prior therapy and pulmonary status, were employed. Twenty-six patients without a history of prior chest or pelvic irradiation were treated with fractionated total body irradiation, etoposide (VP) 60 mg/kg and cyclophosphamide (Cy) 100 mg/kg. Seventy-four patients received BCNU 15 mg/kg with identical doses of VP and Cy. A group of 19 patients with a limited diffusing capacity or history of pneumonitis received a novel high-dose regimen consisting of CCNU 15 mg/kg, VP 60 mg/kg and Cy 100 mg/kg. Twenty-nine patients (24%) had failed induction therapy and 35 (29%) had progressive HD within 1 year of initial chemotherapy. At 4 years actuarial survival was 52%, event-free survival was 48% and freedom from progression (FFP) was 62%. No significant differences were seen in survival data with the three preparatory regimens. Six patients died within 100 days of transplantation and 5 died at a later date of transplant-related complications. Secondary malignancies have developed in 6 patients, including myelodysplasia/leukemia in four patients and solid tumors in two patients. Regression analysis identified systemic symptoms at relapse, disseminated pulmonary or bone marrow disease at relapse and more than minimal disease at the time of transplantation as significant prognostic factors for overall and event-free survival and FFP. Patients with none of these factors enjoyed an 85% FFP at 4 years compared with 41% for patients with one or more unfavorable prognostic factors (P = .0001). Our results confirm the efficacy of high-dose therapy and autografting in recurrent or refractory HD. Although longer follow-up is necessary to address ultimate cure rates and toxicity, our data indicate that a desire to reduce late effects should drive future research efforts in favorable patients whereas new initiatives are needed for those with less favorable prognoses.
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- 1997
33. Thalidomide as salvage therapy for chronic graft-versus-host disease
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Karl G. Blume, Nelson J. Chao, Stephen J. Forman, George Somlo, K. Wilsman, David S. Snyder, Joyce C. Niland, Pablo M. Parker, Robert S. Negrin, Gwynn D. Long, Kim Margolin, K. Zwingenberger, Eileen P. Smith, Ricardo Spielberger, Anthony S. Stein, G M Schmidt, Ashwin Kashyap, Daniel E. Stepan, Auayporn Nademanee, Margaret R. O'Donnell, Ina Planas, and Arturo Molina
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medicine.medical_specialty ,Chemotherapy ,business.industry ,medicine.medical_treatment ,Immunology ,Neuritis ,Salvage therapy ,Cell Biology ,Hematology ,Neutropenia ,medicine.disease ,Biochemistry ,Gastroenterology ,Rash ,Surgery ,Thalidomide ,Graft-versus-host disease ,Prednisone ,hemic and lymphatic diseases ,Internal medicine ,medicine ,medicine.symptom ,business ,medicine.drug - Abstract
Thalidomide has been reported to be an effective agent for treatment of chronic graft-versus-host disease (CGVHD). To determine the efficacy of this agent in patients with refractory CGVHD a total of 80 patients who failed to respond to prednisone (PSE) or PSE and cyclosporine (CSA) were treated with thalidomide. Sixteen patients (20%) had a sustained response, 9 with a complete remission and 7 with a partial response. Twenty-nine patients (36%) had thalidomide discontinued because of side effects, which included sedation, constipation, neuritis, skin rash, and neutropenia. Side effects were reversible with drug discontinuation except for mild residual neuritis in one case. Rashes and neutropenia have not previously been reported as thalidomide side effects when used for CGVHD treatment. We conclude thalidomide is immunosuppressive and active in the treatment of CGVHD. A high incidence of reversible side effects limited dose intensity and reduced the number of patients who could benefit from treatment.
- Published
- 1995
34. Transplantation of enriched and purged peripheral blood progenitor cells from a single apheresis product in patients with non-Hodgkin's lymphoma
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Jeffrey Schriber, Byron W. Brown, Claus R. Kusnierz-Glaz, Nelson J. Chao, BJ Still, Wendy W. Hu, Gwynn D. Long, Robert S. Negrin, Sandra J. Horning, and Christine F. Hoyle
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Pathology ,medicine.medical_specialty ,Neutrophil Engraftment ,business.industry ,medicine.medical_treatment ,Immunology ,CD34 ,Cell Biology ,Hematology ,Hematopoietic stem cell transplantation ,Biochemistry ,medicine.anatomical_structure ,White blood cell ,Absolute neutrophil count ,medicine ,Cancer research ,Autologous transplantation ,Bone marrow ,Progenitor cell ,business - Abstract
High-dose chemotherapy with or without radiotherapy followed by autologous transplantation of hematopoietic progenitor cells is an effective treatment for patients with high-risk or relapsed non- Hodgkin's lymphoma. Chemotherapy and/or hematopoietic growth factors have been used to mobilize progenitor cells in the peripheral blood for transplantation. However, the mobilized blood cell products have been found to be frequently contaminated with tumor cells, and techniques have not been developed to purge tumor cells from these products. In addition, the minimum number of hematopoietic progenitor cells required for engraftment has not yet been fully elucidated. We treated 21 patients with a single infusion of cyclophosphamide (4 g/m2) followed by daily administration of granulocyte colony-stimulating factor (G-CSF). After recovery of the white blood cell count, a single 3-hour apheresis collection was performed. The apheresis product was then applied to a discontinuous Percoll gradient. The low-density fractions resulting from this separation procedure were enriched for CD34+ progenitor cells (total cell yield, 19.5%; CD34+ cell recovery, 81.2%). These enriched cellular products were treated with a panel of anti-B cell or anti-T cell monoclonal antibodies and complement in an effort to remove residual tumor cells. After treatment of the patient with myeloablative therapies, the enriched and purged cells were reinfused. Hematologic recovery was rapid, with median neutrophil engraftment in 10 days [absolute neutrophil count (ANC), greater than 0.5 x 10(9)/L] and 11 days (ANC, greater than 1.0 x 10(9)/L). Median platelet transfusion independence required 13 days. The rapidity of multilineage engraftment correlated with the number of CD34+ cells per kilogram that were infused. Patients who received more than 2 x 10(6) CD34+ cells per kilogram had rapid hematologic engraftment, whereas those patients transplanted with less than 2 x 10(6) CD34+ cells per kilogram had slower platelet recovery. Modeling studies using a lymphoma cell line with a t(14; 18) chromosomal translocation demonstrated the successful removal of tumor cells assayed using the polymerase chain reaction (PCR) after the processing and purging. Four of the 21 patients had PCR-detectable lymphoma cells in the bone marrow and peripheral blood; however, the enriched and purged blood products reinfused in all four did not contain detectable tumor cells.(ABSTRACT TRUNCATED AT 400 WORDS)
- Published
- 1995
35. A Phase I-II Study of the Combination of Bendamustine and Pomalidomide with Dexamethasone in Patients with Relapsed or Refractory Multiple Myeloma
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Anderson Garrett, Sascha A. Tuchman, Zighuo Li, Cristina Gasparetto, Anandgopal Srinivasan, Louis F. Diehl, Arati V. Rao, Yubin Kang, David A. Rizzieri, Michael Green, Carlos M. DeCastro, Gwynn D. Long, and Anne W. Beaven
- Subjects
Bendamustine ,medicine.medical_specialty ,education.field_of_study ,business.industry ,Immunology ,Population ,Cell Biology ,Hematology ,medicine.disease ,Pomalidomide ,Biochemistry ,Thalidomide ,Tolerability ,Internal medicine ,medicine ,Progression-free survival ,business ,education ,Febrile neutropenia ,medicine.drug ,Lenalidomide - Abstract
Background Bendamustine, a bifunctional mechlorethamine derivative with alkylating properties and pomalidomide, an IMiD® immunomodulatory agent, have both demonstrated efficacy as single agents or in combination with dexamethasone in relapsed/refractory multiple myeloma(RRMM). Bendamustine in combination with lenalidomide, thalidomide, and bortezomib have had high response rates and good tolerability. We combined bendamustine and pomalidomide with dexamethasone (Ben-Pom-d) and hypothesized that this regimen would be highly effective in patients with RRMM. Dose-escalation started with 120mg/m2 bendamustine/3mg pomalidomide [or 4mg in the cohort 2]/40 mg dexamethasone using a standard 3+3 schema based on dose-limiting toxicities (DLTs) occurring in cycle 1. The MTD was 120mg/m2 bendamustine/3mg pomalidomide/40 mg dexamethasone. Here, we report our findings to date from the phase I/II trial of Ben-Pom-d in patients with RRMM (NCT01754402). Methods The primary objective of the phase I portion was to determine the MTD. Data for overall response, progression free survival, and overall survival, includes all patients treated on the phase I and II portions of the study. All patients had to be refractory to prior lenalidomide, and must have relapsed or were refractory to their most recent therapy. Patients had to be pomalidomide naïve. Treatment consisted of oral pomalidomide once daily on days 1-21, intravenous (IV) bendamustine given over 30 minutes on day 1 and dexamethasone 40 mg on days 1, 8, 15, and 22 of a 28-day cycle. Adverse events (AEs) were graded by NCI-CTCAE v4. Response was assessed by the modified International Uniform Response Criteria. Results A total of 9 patients were enrolled in the phase I portion. The MTD was the starting dose level (bendamustine 120 mg/m2, pomalidomide 3mg, dexamethasone 40 mg). In Phase II we enrolled an additional 16 patients resulting in a total study population of 25 patients evaluable for toxicity and 22 for efficacy, with 6 still receiving treatment. The median age was 65 years (range 43-81), 46% were male. The median number of prior regimens was 3 (range 2-6), median time from diagnosis is 3.9 years (range 1.1-9.10 years), 88% of patients had a prior stem cell transplant, 100% had prior bortezomib, 20% had prior carfilzomib and all were lenalidomide-refractory. Fifteen patients had high risk cytogenetic, including 8 patients with del17. Patients received a median of 6 cycles of therapy (range 1-18 cycles). Best response assessments in 22 evaluable patients for efficacy, showed 5 (23%) VGPR, 12 (55%) PR, 3 (14%) MR, and 2 (9%) SD, for an ORR of 77% and a ≥MR rate of 91%. The median follow-up of survivors is 10 months (range: 2-19+ months). Median PFS and OS were 4.5 months (range 1-15+ months) and 9.5 months (range 2-19+ months), respectively, for the entire cohort with 13 of 22 still alive in follow-up. The Median PFS for patient with del 17 is 5.5 months (range 2-15 months) with >MR rate of 88%. During the first cycle, 3 patients of all 25 evaluable enrolled experienced a DLT at the different doses, including 1 nausea/vomiting [cohort 1], and 2 with rash and fever in cohort 2. The therapy was tolerated well, but toxicities reported at any point while on therapy included 32% grade 4 neutropenia, 16% grade 4 thrombocytopenia, and half the patients requiring delay of subsequent cycles due to cytopenias and 17 of 22 (77%) had a dose reduction of pomalidomide per protocol guidelines at some point in the continuation cycles. The major non-hematologic Grade ≥3 drug-related AEs that occurred included febrile neutropenia in 12%, grade 3 mucositis in 8%, grade 3 pneumonia 16% and grade 4%, and grade 4 sepsis 4%. Conclusions The Ben-Pom-d regimen is a well-tolerated regimen and achieves a high response rate (ORR of 77%; ≥MR rate of 91%) in a heavily pre-treated Lenalidomide-refractory population with prior bortezomib exposure. Therapy is ongoing for many and longer follow-up is needed to better assess the true durability of this approach. Disclosures Gasparetto: Onyx: Honoraria, Other: Advisory Board; Millennium/takeda: Honoraria, Membership on an entity's Board of Directors or advisory committees, Speakers Bureau; celgene: Honoraria, Membership on an entity's Board of Directors or advisory committees, Research Funding, Speakers Bureau; teva: Other: spouse-ad board and speaker bureau. Off Label Use: Bendamustine-pomalidomide-dexa for treatment of relapsed myeloma. Rizzieri:Teva: Other: ad board, Speakers Bureau; Celgene: Other: ad board, Speakers Bureau. Rao:novartis: Other: ad board; amgen: Other: ad board; Boehringer-Ingelheim: Other: Advisory Board. Tuchman:celgene: Consultancy, Honoraria, Research Funding, Speakers Bureau; Millennium/takeda: Honoraria, Research Funding, Speakers Bureau.
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- 2015
36. Fractionated total-body irradiation and high-dose etoposide as a preparatory regimen for bone marrow transplantation for 94 patients with chronic myelogenous leukemia in chronic phase
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Eileen P. Smith, Pablo M. Parker, Anthony S. Stein, Robert S. Negrin, O'Donnell, Amylon, David S. Snyder, A P Nademanee, Gwynn D. Long, and Nelson J. Chao
- Subjects
Chemotherapy ,medicine.medical_specialty ,Prognostic variable ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Total body irradiation ,medicine.disease ,Biochemistry ,Gastroenterology ,Surgery ,Regimen ,medicine.anatomical_structure ,Internal medicine ,medicine ,Bone marrow ,business ,Busulfan ,Etoposide ,medicine.drug ,Chronic myelogenous leukemia - Abstract
Ninety-four consecutive patients with chronic myelogenous leukemia in first clinical chronic phase, median age of 34.0 years (range, 6.8 to 52.4 years), with a histocompatible sibling donor, were treated with fractionated total body irradiation (1,320 cGy) and high-dose etoposide (60 mg/kg) followed by allogeneic bone marrow transplantation (BMT). The median time from diagnosis to BMT was 7.0 months (range, 2.3 to 72.0 months). Sixty patients were treated before BMT with hydroxyurea alone, four patients with busulfan alone, one patient with interferon alone, and the other 29 patients were treated with various combinations of these drugs. Cumulative probabilities of overall survival, event- free survival, and relapse at 5 years were 73%, 64%, and 14%, respectively. The median follow-up time for surviving patients was 38 months, ranging from 12 to 88 months. By stepwise Cox regression analysis, significant prognostic variables were age at transplant, acute graft-versus-host disease > or = grade II, cytomegalovirus- associated interstitial pneumonitis, and years from diagnosis to BMT.
- Published
- 1994
37. Granulocyte colony-stimulating factor after allogeneic bone marrow transplantation
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Blume Kg, Gwynn D. Long, Jeffrey Schriber, Claus R. Kusnierz-Glaz, Nelson J. Chao, KS Lucas, DK Tierney, and Robert S. Negrin
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medicine.medical_specialty ,Myeloid ,Immunology ,chemical and pharmacologic phenomena ,Neutropenia ,Biochemistry ,Gastroenterology ,immune system diseases ,Prednisone ,hemic and lymphatic diseases ,Internal medicine ,medicine ,business.industry ,Cell Biology ,Hematology ,medicine.disease ,Granulocyte colony-stimulating factor ,Regimen ,Leukemia ,surgical procedures, operative ,medicine.anatomical_structure ,Absolute neutrophil count ,Bone marrow ,business ,medicine.drug - Abstract
Hematopoietic growth factors have been shown to be effective in reducing the period of neutropenia after autologous bone marrow transplantation (BMT). Initial concerns over potential aggravation of graft-versus-host disease (GVHD) and increase in the incidence of relapse in patients with myeloid leukemias influenced the number of studies using hematopoietic growth factors after allogeneic BMT. We report the experience with 50 patients treated at a single institution using granulocyte colony-stimulating factor (G-CSF) after allogeneic sibling (n = 30) and matched unrelated (n = 20) BMT. The time to an absolute neutrophil count > or = 500/microL was significantly faster in patients who received G-CSF and cyclosporine and prednisone for GVHD prophylaxis when compared with historical control patients receiving the same GVHD prophylaxis (10 v 13 days, P < .01). A similar accelerated myeloid engraftment was observed for those patients who received the addition of methotrexate for GVHD prophylaxis when compared with historical control patients receiving the same GVHD prophylaxis regimen (16 v 19 days, P < .05). The median time to engraftment for patients receiving a matched unrelated BMT and G-CSF was 17 days (range 13 to 26). We did not observe any increase in GVHD or early mortality in the matched related sibling BMT. The incidence of acute GVHD in the matched unrelated BMT recipients was also low at 21%; however, 9 patients (45%) died within 100 days of the date of BMT, similar to the experience reported with granulocyte-macrophage CSF. This study confirms the efficacy of G-CSF in accelerating myeloid engraftment after allogeneic matched sibling BMT. The higher early mortality associated with patients receiving matched unrelated BMT suggests that randomized controlled trials using G-CSF after allogeneic BMT should be performed.
- Published
- 1994
38. A randomized study of erythropoietin and granulocyte colony-stimulating factor (G-CSF) versus placebo and G-CSF for patients with Hodgkin's and non-Hodgkin's lymphoma undergoing autologous bone marrow transplantation
- Author
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Marbel Catolico, Karl G. Blume, Jeffrey Schriber, Langdon L. Miller, Nelson J. Chao, Byron W. Brown, Robert S. Negrin, and Gwynn D. Long
- Subjects
medicine.medical_specialty ,business.industry ,Anemia ,Immunology ,Cell Biology ,Hematology ,medicine.disease ,Placebo ,Biochemistry ,Gastroenterology ,Surgery ,Granulocyte colony-stimulating factor ,Non-Hodgkin's lymphoma ,Transplantation ,medicine.anatomical_structure ,Erythropoietin ,hemic and lymphatic diseases ,Internal medicine ,Absolute neutrophil count ,Medicine ,Bone marrow ,business ,medicine.drug - Abstract
Anemia is a universal finding in patients undergoing autologous bone marrow transplantation (BMT). Effective therapies to increase the number of autologous red blood cells could result in a lower morbidity and mortality associated with red blood cell transfusions. We examined whether the addition of erythropoietin (Epo) to intensive therapy supported by progenitor cell transplantation and granulocyte colony- stimulating factor (G-CSF) would result in a lower requirement for red blood cell transfusions. Thirty-five patients with lymphoma were randomized to receive Epo versus placebo. Epo (600 U/kg three times per week) or placebo was begun 3 weeks before administration of high-dose therapy. Epo was held during the week of the preparatory regimen, and restarted on the day after BMT. All patients also received G-CSF following BMT. No significant differences were noted between the two groups in terms of patient characteristics at pretreatment or post-BMT evaluation. There were no differences in the total number of red blood cell units transfused (median Epo: 8 v placebo: 6, P = .22) nor the number of platelet transfusions given (median Epo: 12 v placebo 5, P = .14). Engraftment of granulocytes (absolute neutrophil count > or = 500/microL) occurred in a median of 12 days (range, 9 to 33) for the patients receiving Epo and G-CSF, compared with a median of 10 days (range, 8 to 22) for those receiving placebo and G-CSF (P = .70). Likewise, there were no differences in the time to platelet count > or = 20,000/microL without further transfusions with a median of 22 days (range, 15 to 150+) for those receiving Epo and G-CSF compared with a median of 20 days (range, 11 to 54) for those patients receiving placebo and G-CSF (P = .28). The combination of G-CSF and Epo as administered in this study appears to be safe but does not result in an improvement in the total number of red blood cell transfusions or total number of single donor platelet units transfused.
- Published
- 1994
39. Fractionated total body irradiation and high-dose etoposide as a preparatory regimen for bone marrow transplantation for 99 patients with acute leukemia in first complete remission
- Author
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David S. Snyder, O'Donnell, J Taguchi, Anthony S. Stein, Gwynn D. Long, Amylon, Nelson J. Chao, A P Nademanee, Robert S. Negrin, and G M Schmidt
- Subjects
Acute leukemia ,medicine.medical_specialty ,Chemotherapy ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Total body irradiation ,medicine.disease ,Biochemistry ,Gastroenterology ,Surgery ,Leukemia ,Myelogenous ,medicine.anatomical_structure ,hemic and lymphatic diseases ,Internal medicine ,medicine ,Acute Undifferentiated Leukemia ,Bone marrow ,business ,Etoposide ,medicine.drug - Abstract
Ninety-nine consecutive patients with acute leukemia in first complete remission under age 50 (median age 27 years; age range 1 to 47 years) with a histocompatible sibling donor were treated with fractionated total body irradiation (1,320 cGy) and high-dose etoposide (60 mg/kg) followed by allogeneic bone marrow transplantation. Sixty-one patients were diagnosed with acute myelogenous leukemia (AML), 34 patients with acute lymphoblastic leukemia (ALL), 3 patients with biphenotypic acute leukemia, and 1 patient with acute undifferentiated leukemia. Thirty of the 34 patients with ALL had at least one of the following high-risk factors: age greater than 30, white blood cell count at presentation > 25,000/microL, extramedullary disease, certain chromosomal translocations, or the need for greater than 4 weeks of induction chemotherapy to achieve first complete remission. Cumulative probabilities of disease-free survival and relapse at 3 years were 61% and 12%, respectively, for the 61 patients with AML and 64% and 12%, respectively, for the 34 patients with ALL. By stepwise Cox regression analysis, significant prognostic variables for patients with acute myelogenous leukemia were the presence of acute graft-versus-host disease and increasing age, whereas for patients with acute lymphoblastic leukemia, significant variables were age and the development of cytomegalovirus-associated interstitial pneumonia. Complications related to graft-versus-host disease and relapse of leukemia were the major causes of death.
- Published
- 1993
40. Granulocyte colony-stimulating factor 'mobilized' peripheral blood progenitor cells accelerate granulocyte and platelet recovery after high-dose chemotherapy
- Author
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Robert S. Negrin, Nelson J. Chao, Cathleen M. Raimondi, Gwynn D. Long, Sandra J. Horning, Sherri L. Brown, Jeffrey Schriber, Karl G. Blume, Langdon L. Miller, and Kevin Grimes
- Subjects
medicine.medical_specialty ,Chemotherapy ,Platelet Engraftment ,business.industry ,medicine.medical_treatment ,Immunology ,Cell Biology ,Hematology ,Granulocyte ,Biochemistry ,Granulocyte colony-stimulating factor ,Haematopoiesis ,Endocrinology ,medicine.anatomical_structure ,Internal medicine ,medicine ,Platelet ,Progenitor cell ,Stem cell ,business - Abstract
Hematopoietic growth factors have been used to accelerate engraftment after bone marrow transplantation and to “mobilize” peripheral blood progenitor cells (PBPC). We report on the data in 85 consecutive patients with Hodgkin's disease who were treated in a single institution using different methods to obtain PB progenitor cells. Use of granulocyte colony-stimulating factor for mobilization resulted in a significantly accelerated time to recovery of granulocytes (10 days v 12 days, P < .01) when compared with “nonmobilized” PBPC recipients. Similarly, use of mobilized PBPC resulted in a significantly accelerated time to platelet engraftment (13 days v 30 days, P < .001) when compared with “nonmobilized” recipients. Moreover, there was a statistically significant difference in total costs in favor of the group receiving “mobilized” PBPC.
- Published
- 1993
41. Busulfan/etoposide--initial experience with a new preparatory regimen for autologous bone marrow transplantation in patients with acute nonlymphoblastic leukemia
- Author
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Ruby M. Wong, Robert S. Negrin, Gwynn D. Long, Michael D. Amylon, Nelson J. Chao, Karl G. Blume, Anthony S. Stein, and Stephen J. Forman
- Subjects
medicine.medical_specialty ,business.industry ,Immunology ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Bone marrow purging ,Surgery ,Transplantation ,Regimen ,Leukemia ,medicine ,Absolute neutrophil count ,business ,Etoposide ,Busulfan ,medicine.drug ,Preparative Regimen - Abstract
Current intensive chemotherapy for acute nonlymphoblastic leukemia (ANLL) results in a complete remission in the majority of patients. Unfortunately, the duration of remission is short and most of the patients will experience a relapse of their underlying disease. Autologous bone marrow (BM) transplantation is being explored as a treatment modality designed to improve relapse-free survival. We have conducted a phase II trial exploring the combination of busulfan (16 mg/kg) and etoposide (60 mg/kg) in an attempt to improve antitumor efficacy using this novel preparative regimen. To date, 50 patients (48 with ANLL and 2 patients with biphenotypic acute leukemia) have been treated. The first 20 patients received unmanipulated BM; 28 patients subsequently received 4-hydroperoxycyclophosphamide (4–HC) (60 micrograms/mL)-purged bone marrow, and 2 patients with biphenotypic acute leukemia received both 4–HC (60 micrograms/mL) and etoposide (5 micrograms/mL)-purged BM. Thirty-four patients were in first complete remission (CR1), 12 patients in second complete remission (CR2), and 4 patients in relapse. The median time from first complete remission to BM harvest was 3 months (range, 0.8 to 4) compared with median time of 2 months (range, 1.5 to 5.0) for patients in second complete remission. The median time from harvest to transplant was 1 month for both groups (range, 0.4 to 36). A median of 0.7 x 10(8) (range, 0.2 to 1.4) mononuclear cells were infused. Patients achieved an absolute neutrophil count of > or = 500/microL at a median of 26 days (range, 13 to 96), an untransfused platelet count > or = 20,000/microL at a median of 56 days (range, 15 to 278) and a sustained hematocrit > or = 30% at a median of 50 days (range, 19 to 116). Twenty-six patients are alive and in continued CR. Follow-up of the surviving patients ranged from 6 months to 66 months with a median follow-up of 31 months. Patients receiving purged BM have an actuarial disease-free survival of 57% with a relapse rate of 28% compared with patients receiving unpurged BM whose actuarial disease-free survival is 32% with a relapse rate of 62% (P = .06 for relapse rate). The most significant extramedullary toxicities for this regimen are hepatic and cutaneous (including mucositis). The BU/VP-16 regimen is associated with a significant proportion of patients surviving disease free, especially in the group receiving purged BM. Whether this regimen offers a substantial improvement in disease-free survival over currently used regimens will require a prospective randomized study.
- Published
- 1993
42. Increased life span and correction of metabolic defects in murine mucopolysaccharidosis type VII after syngeneic bone marrow transplantation
- Author
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J W Kyle, Carole Vogler, Babette Gwynn, Edward H. Birkenmeier, Jane E. Barker, Beth Levy, William S. Sly, and Catherine Pegors
- Subjects
Pathology ,medicine.medical_specialty ,Ratón ,Mucopolysaccharidosis ,Glomerular Mesangial Cell ,Immunology ,Sly syndrome ,Spleen ,Mucopolysaccharidosis VII ,Cell Biology ,Hematology ,Biology ,Syngeneic Bone Marrow Transplantation ,medicine.disease ,Biochemistry ,medicine.anatomical_structure ,medicine ,Bone marrow - Abstract
The gusmps/gusmps mouse has no beta-glucuronidase activity and develops murine mucopolysaccharidosis type VII (MPS VII). The clinical and pathologic abnormalities are similar to those found in humans with severe MPS VII. Mutant mice are dysmorphic, dwarfed, and have a shortened life span. Pathologic findings include widespread lysosomal storage. To determine whether bone marrow transplantation (BMT) corrects these abnormalities, genetically identical mutant animals were given syngeneic bone marrow transplants using cells from +/+ mice. Initial experiments showed that levels of beta-glucuronidase activity in recipient tissues correlated with the amount of radiation administered before BMT. Two groups of mice given BMT therapy were observed for periods of 1 and 2 years, respectively. These mice were evaluated using a combination of clinical, biochemical, histochemical, and pathologic analyses. Spleen, liver, cornea, and glomerular mesangial cells showed essentially complete correction at all radiation doses. Storage was partially corrected in meninges and perivascular cells in brain, and in renal tubular epithelial cells at the higher radiation doses. Life span in BMT-treated animals was increased approximately three-fold, approaching that seen in normal mice after BMT. These results support the position that BMT has a place in the therapeutic regimen for MPS VII.
- Published
- 1991
43. Increased life span and correction of metabolic defects in murine mucopolysaccharidosis type VII after syngeneic bone marrow transplantation
- Author
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E H, Birkenmeier, J E, Barker, C A, Vogler, J W, Kyle, W S, Sly, B, Gwynn, B, Levy, and C, Pegors
- Subjects
Immunology ,Dose-Response Relationship, Radiation ,Cell Biology ,Hematology ,Mucopolysaccharidoses ,Survival Analysis ,Biochemistry ,Mice, Mutant Strains ,Mice ,Microscopy, Electron ,surgical procedures, operative ,Animals ,Lysosomes ,Whole-Body Irradiation ,Bone Marrow Transplantation ,Glucuronidase ,Glycosaminoglycans ,Granulocytes - Abstract
The gusmps/gusmps mouse has no beta-glucuronidase activity and develops murine mucopolysaccharidosis type VII (MPS VII). The clinical and pathologic abnormalities are similar to those found in humans with severe MPS VII. Mutant mice are dysmorphic, dwarfed, and have a shortened life span. Pathologic findings include widespread lysosomal storage. To determine whether bone marrow transplantation (BMT) corrects these abnormalities, genetically identical mutant animals were given syngeneic bone marrow transplants using cells from +/+ mice. Initial experiments showed that levels of beta-glucuronidase activity in recipient tissues correlated with the amount of radiation administered before BMT. Two groups of mice given BMT therapy were observed for periods of 1 and 2 years, respectively. These mice were evaluated using a combination of clinical, biochemical, histochemical, and pathologic analyses. Spleen, liver, cornea, and glomerular mesangial cells showed essentially complete correction at all radiation doses. Storage was partially corrected in meninges and perivascular cells in brain, and in renal tubular epithelial cells at the higher radiation doses. Life span in BMT-treated animals was increased approximately three-fold, approaching that seen in normal mice after BMT. These results support the position that BMT has a place in the therapeutic regimen for MPS VII.
- Published
- 1991
44. Targeted deletion of alpha-adducin results in absent beta- and gamma-adducin, compensated hemolytic anemia, and lethal hydrocephalus in mice
- Author
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Kenneth E. Sahr, Babette Gwynn, Diana M. Gilligan, Raymond F. Robledo, Narla Mohandas, Luanne L. Peters, and Steven L. Ciciotte
- Subjects
Hemolytic anemia ,Blood Platelets ,Immunology ,Spherocytosis ,Red Cells ,macromolecular substances ,Spherocytosis, Hereditary ,Biology ,Microfilament ,Anemia, Hemolytic, Congenital ,Biochemistry ,Hereditary spherocytosis ,Mice ,Spherocytes ,medicine ,Animals ,Spectrin ,Cytoskeleton ,education ,Protein Structure, Quaternary ,Mice, Knockout ,education.field_of_study ,Elliptocytes ,Cell Biology ,Hematology ,medicine.disease ,Molecular biology ,Actins ,Red blood cell ,Cytoskeletal Proteins ,Osmotic Fragility ,medicine.anatomical_structure ,Gene Deletion ,Hydrocephalus - Abstract
In the red blood cell (RBC), adducin is present primarily as tetramers of alpha- and beta-subunits at spectrin-actin junctions, or junctional complexes. Mouse RBCs also contain small amounts of gamma-adducin. Platelets contain alpha- and gamma-adducin only. Adducin functions as a barbed-end actin capping protein to regulate actin filament length and recruits spectrin to the ends of actin filaments. To further define adducin's role in vivo, we generated alpha-adducin knockout mice. alpha-Adducin is absent in all tissues examined in homozygous null mice. In RBCs, beta- and gamma-adducin are also absent, indicating that alpha-adducin is the limiting subunit in tetramer formation at the spectrin-actin junction. Similarly, gamma-adducin is absent in alpha-null platelets. alpha-Adducin-null mice display compensated hemolytic anemia with features characteristic of RBCs in hereditary spherocytosis (HS), including spherocytes with significant loss of surface area, decreased mean corpuscular volume (MCV), cell dehydration, and increased osmotic fragility. Platelets maintain their normal discoid shape, and bleeding times are normal. alpha-Adducin-null mice show growth retardation at birth and throughout adulthood. Approximately 50% develop lethal communicating hydrocephalus with striking dilation of the lateral, third, and fourth ventricles. These data indicate that adducin plays a role in RBC membrane stability and in cerebrospinal fluid homeostasis.
- Published
- 2008
45. Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex
- Author
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Juan S. Bonifacino, M. Lynn Lamoreux, Jose A. Martina, William A. Gahl, Lisa S. Webb, Marjan Huizing, Kengo Moriyama, Amanda Helip-Wooley, Elena V. Sviderskaya, Amy J. Lambert, Luanne L. Peters, Dorothy C. Bennett, and Babette Gwynn
- Subjects
Male ,Adaptor Protein Complex 3 ,Vesicle docking ,Immunology ,Molecular Sequence Data ,HPS5 ,Nerve Tissue Proteins ,Biology ,medicine.disease_cause ,Biochemistry ,Mice ,hemic and lymphatic diseases ,Cell Line, Tumor ,Organelle ,medicine ,Animals ,Humans ,Adaptor Protein Complex beta Subunits ,Amino Acid Sequence ,Cloning, Molecular ,Melanoma ,Mutation ,Pigmentation ,Chromosome Mapping ,Membrane Transport Proteins ,Cell Biology ,Hematology ,Fibroblasts ,medicine.disease ,Molecular biology ,eye diseases ,Mice, Mutant Strains ,Mice, Inbred C57BL ,Disease Models, Animal ,Phenotype ,Hermanski-Pudlak Syndrome ,Melanocytes ,Female ,sense organs ,Organelle biogenesis ,Hermansky–Pudlak syndrome ,Carrier Proteins ,Lysosomes ,Biogenesis ,Transcription Factors - Abstract
Hermansky-Pudlak syndrome (HPS), a disorder of organelle biogenesis, affects lysosomes, melanosomes, and platelet dense bodies. Seven genes cause HPS in humans (HPS1-HPS7) and at least 15 nonallelic mutations cause HPS in mice. Where their function is known, the HPS proteins participate in protein trafficking and vesicle docking/fusion events during organelle biogenesis. HPS-associated genes participate in at least 4 distinct protein complexes: the adaptor complex AP-3; biogenesis of lysosome-related organelles complex 1 (BLOC-1), consisting of 4 HPS proteins (pallidin, muted, cappuccino, HPS7/sandy); BLOC-2, consisting of HPS6/ruby-eye, HPS5/ruby-eye-2, and HPS3/cocoa; and BLOC-3, consisting of HPS1/pale ear and HPS4/light ear. Here, we report the cloning of the mouse HPS mutation reduced pigmentation (rp). We show that the wild-type rp gene encodes a novel, widely expressed 195-amino acid protein that shares 87% amino acid identity with its human orthologue and localizes to punctate cytoplasmic structures. Further, we show that phosphorylated RP is part of the BLOC-1 complex. In mutant rp/rp mice, a premature stop codon truncates the protein after 79 amino acids. Defects in all the 5 known components of BLOC-1, including RP, cause severe HPS in mice, suggesting that the subunits are nonredundant and that BLOC-1 plays a key role in organelle biogenesis.
- Published
- 2004
46. Cappuccino, a mouse model of Hermansky-Pudlak syndrome, encodes a novel protein that is part of the pallidin-muted complex (BLOC-1)
- Author
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Steven L. Ciciotte, Babette Gwynn, Luanne L. Peters, Juan S. Bonifacino, William A. Gahl, Marjan Huizing, and Kengo Moriyama
- Subjects
Biogenesis of lysosome-related organelles complex 1 ,Vesicle docking ,Immunology ,Mutant ,DNA Mutational Analysis ,Molecular Sequence Data ,Vesicular Transport Proteins ,Biology ,medicine.disease_cause ,Biochemistry ,Frameshift mutation ,Mice ,hemic and lymphatic diseases ,Lectins ,Protein targeting ,medicine ,Animals ,Humans ,Tissue Distribution ,Amino Acid Sequence ,Genetics ,Mutation ,Intracellular Signaling Peptides and Proteins ,Cell Biology ,Hematology ,Fibroblasts ,medicine.disease ,eye diseases ,Hermanski-Pudlak Syndrome ,Organelle biogenesis ,Hermansky–Pudlak syndrome ,Carrier Proteins ,Protein Binding - Abstract
Hermansky-Pudlak syndrome (HPS) is a disorder of organelle biogenesis affecting 3 related organelles—melanosomes, platelet dense bodies, and lysosomes. Four genes causing HPS in humans (HPS1-HPS4) are known, and at least 15 nonallelic mutations cause HPS in the mouse. Where their functions are known, the HPS-associated proteins are involved in some aspect of intracellular vesicular trafficking, that is, protein sorting and vesicle docking and fusion. Biochemical and genetic evidence indicates that the HPS-associated genes encode components of at least 3 distinct protein complexes: the adaptor complex AP-3; the HPS1/HPS4 complex; and BLOC-1 (biogenesis of lysosome-related organelles complex-1), consisting of the proteins encoded at 2 mouse HPS loci, pallid (pa) and muted (mu), and at least 3 other unidentified proteins. Here, we report the cloning of the mouse HPS mutation cappuccino (cno). We show that the wild-type cno gene encodes a novel, ubiquitously expressed cytoplasmic protein that coassembles with pallidin and the muted protein in the BLOC-1 complex. Further, we identify a frameshift mutation in mutant cno/cno mice. The C-terminal 81 amino acids are replaced with 72 different amino acids in the mutant CNO protein, and its ability to interact in BLOC-1 is abolished. We performed mutation screening of patients with HPS and failed to identify any CNO defects. Notably, although defects in components of the HPS1/HPS4 and the AP-3 complexes are associated with HPS in humans, no defects in the known components of BLOC-1 have been identified in 142 patients with HPS screened to date, suggesting that BLOC-1 function may be critical in humans.
- Published
- 2003
47. Tmem14c Plays An Essential Role In Mitochondrial Heme Metabolism
- Author
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Paw, Barry H., primary, Yien, Yvette Y., additional, Robledo, Raymond F, additional, Schultz, Iman J., additional, Takahashi-Makise, Naoko, additional, Philllips, John, additional, Miyata, Non, additional, Gwynn, Babette, additional, Dailey, Tamara A., additional, Pierce, Eric, additional, Hildick-Smith, Gordon J., additional, Cooney, Jeffrey D., additional, Chen, Wen, additional, Shah, Dhvanit I., additional, Hattangadi, Shilpa M, additional, Kingsley, Paul D, additional, Palis, James, additional, Cantor, Alan B., additional, Koehler, Carla M., additional, Lodish, Harvey F., additional, Kaplan, Jerry, additional, Dailey, Harry A., additional, McVey Ward, Diane, additional, and Peters, Luanne L., additional
- Published
- 2013
- Full Text
- View/download PDF
48. Successful allogeneic engraftment of mismatched unrelated cord blood following a nonmyeloablative preparative regimen
- Author
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Timothy T. Stenzel, Patti Davis, David A. Rizzieri, C. Gasparetto, James J. Vredenburgh, Ashley Morris, Gwynn D. Long, and Nelson J. Chao
- Subjects
Adult ,Male ,medicine.medical_specialty ,Allogeneic transplantation ,Transplantation Conditioning ,medicine.medical_treatment ,Immunology ,Hematopoietic stem cell transplantation ,Lymphoma, Mantle-Cell ,Biochemistry ,Umbilical cord ,medicine ,Humans ,Transplantation, Homologous ,Preparative Regimen ,business.industry ,Graft Survival ,Remission Induction ,Hematopoietic Stem Cell Transplantation ,Cell Biology ,Hematology ,Middle Aged ,Fetal Blood ,Tissue Donors ,Surgery ,Histocompatibility ,Transplantation ,medicine.anatomical_structure ,Cord blood ,Lymphoma, Large B-Cell, Diffuse ,business - Abstract
Reduction in the toxicity of allogeneic transplantation with nonmyeloablative induction regimens has expanded the scope of practice to older and more debilitated patients. However, the limited availability of matched sibling donors requires that alternative donor sources be investigated. Reported here are 2 cases of patients with advanced hematologic malignancies without matched siblings, partially matched family members, or matched unrelated donors who successfully underwent nonmyeloablative conditioning therapy followed by infusion of partially matched, unrelated-donor cord blood cells. The patients are in remission and remain 100% donor as assessed by short tandem repeat analysis of the marrow 6 and 12 months following transplantation.
- Published
- 2001
49. High-dose therapy and autologous bone marrow transplantation for follicular lymphoma in first complete or partial remission: results of a phase II clinical trial
- Author
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Richard T. Hoppe, Saul A. Rosenberg, B. William Brown, Karl G. Blume, Sandra J. Horning, Robert S. Negrin, Nelson J. Chao, and Gwynn D. Long
- Subjects
Oncology ,Adult ,Male ,medicine.medical_specialty ,Neoplasm, Residual ,Cyclophosphamide ,medicine.medical_treatment ,Immunology ,Follicular lymphoma ,Biochemistry ,Transplantation, Autologous ,Disease-Free Survival ,Cohort Studies ,Actuarial Analysis ,Recurrence ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Prospective Studies ,Lymphoma, Follicular ,Etoposide ,Bone Marrow Transplantation ,Chemotherapy ,business.industry ,Remission Induction ,Cell Biology ,Hematology ,Total body irradiation ,Middle Aged ,medicine.disease ,Chemotherapy regimen ,Combined Modality Therapy ,Surgery ,Transplantation ,Survival Rate ,medicine.anatomical_structure ,Vincristine ,Prednisone ,Female ,Radiotherapy, Adjuvant ,Bone marrow ,business ,medicine.drug ,Follow-Up Studies - Abstract
Advanced stage follicular small cleaved and mixed cell lymphoma is characterized by relapse from remission and survival ranging from 6 to 12 years. Because young patients have the greatest compromise in longevity, the efficacy and toxicity of high-dose radiochemotherapy and bone marrow transplantation after conventional chemotherapy was evaluated in a prospective phase II clinical trial. Thirty-seven patients in a minimal disease state after conventional chemotherapy received fractionated total body irradiation and high-dose etoposide and cyclophosphamide, followed by purged autologous bone marrow. A reference sample of 188 patients of similar age, stage, and histology managed at this institution before 1988 was identified for comparison of patient characteristics and outcomes. Compared with reference patients, transplant recipients had a higher tumor burden at diagnosis. With a median follow-up of 6.5 years, the estimated 10-year survival after transplantation was 86%. There was a single lymphoma death yielding a 10-year disease-specific survival of 97%. There were 2 early transplant-related deaths and 2 late acute leukemia deaths. Ten patients relapsed, one with microscopic disease only. High tumor burden at diagnosis and incomplete response to chemotherapy adversely influenced survival in the reference but not in the transplanted patients. The estimated risk of death of 14% and relapse of 30% at 10 years in our transplanted follicular lymphoma patients, the majority of whom had high tumor burdens, compares favorably with our observations in appropriately matched reference patients.
- Published
- 2001
50. Tmem14c Plays An Essential Role In Mitochondrial Heme Metabolism
- Author
-
Barry H. Paw, Yvette Y. Yien, Raymond F Robledo, Iman J. Schultz, Naoko Takahashi-Makise, John Philllips, Non Miyata, Babette Gwynn, Tamara A. Dailey, Eric Pierce, Gordon J. Hildick-Smith, Jeffrey D. Cooney, Wen Chen, Dhvanit I. Shah, Shilpa M Hattangadi, Paul D Kingsley, James Palis, Alan B. Cantor, Carla M. Koehler, Harvey F. Lodish, Jerry Kaplan, Harry A. Dailey, Diane McVey Ward, and Luanne L. Peters
- Subjects
Protoporphyrin IX ,Membrane transport protein ,Immunology ,Cell Biology ,Hematology ,Biology ,medicine.disease ,Biochemistry ,Coproporphyrinogen III ,Protoporphyrinogen IX ,Coproporphyrinogen Oxidase ,chemistry.chemical_compound ,Porphyria ,chemistry ,medicine ,biology.protein ,Protoporphyrinogen oxidase ,Heme - Abstract
Red cells synthesize large amounts of heme during terminal differentiation. Central to this process is the transport and trafficking of heme synthesis intermediates within the cell. Despite the importance of transport during heme synthesis, the molecules involved in this process are largely unknown. In a screen for genes that are upregulated during erythroid terminal differentiation, we identified Tmem14c, a predicted multi-pass transmembrane protein as an essential component of the porphyrin metabolism pathway. Here, we report that Tmem14c facilitates the synthesis of mitochondrial protoporphyrin IX from coproporphyrinogen III and is thus required for heme synthesis. Tmem14c is a mitochondrial inner-membrane protein enriched in vertebrate hematopoietic tissues and is required for terminal erythropoiesis. Tmem14c gene-trap mouse embryos are severely anemic and mostly die by E13.5 (Fig. A). Fetal liver erythroid cells derived from gene-trap embryos experience maturation arrest. shRNA silencing of Tmem14c in Friend murine erythroleukemia (MEL) cells results in a significant decrease in de-novo heme synthesis. The biochemical defect is due to a decrease in mitochondrial protoporphyrin IX synthesis, while cytoplasmic porphyrin levels remain normal (Fig. B). The heme synthesis defect in Tmem14c-silenced MEL cells is complemented with a protoporphyrin IX analog. These data show the role of Tmem14c in regulating the terminal steps in mitochondrial porphyrin trafficking. Our findings collectively demonstrate that Tmem14c is required for the transport of mitochondrial porphyrins in developing erythroid cells. Due to its inner-mitochondrial localization and its relative proximity to heme synthetic enzymes coproporphyrinogen oxidase and protoporphyrinogen oxidase (Rhee et al., 2013 Science), Tmem14c can function as a molecular adaptor that facilitates the interaction of proteins involved in porphyrin transport, or as a protoporphyrinogen IX transporter (Fig. C). The identification of Tmem14c as an essential regulator of porphyrin transport and heme synthesis provides a novel genetic tool for exploring erythropoiesis and disorders of heme synthesis such as porphyria and anemia. Disclosures: No relevant conflicts of interest to declare.
- Published
- 2013
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