1. Chromosome aberrations detected by conventional karyotyping using novel mitogens in chronic lymphocytic leukemia with 'normal' FISH: correlations with clinicobiologic parameters
- Author
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Massimo Negrini, Alessia Dalsass, Luca Formigaro, Piero Galieni, Francesca Mestichelli, Elisa Tammiso, Francesco Zaja, Lara Rizzotto, Antonella Bardi, Sara Martinelli, Antonio Cuneo, Renato Fanin, Maria Ciccone, Nicoletta Testa, Ilaria Nichele, Francesca Cibien, Giovanni Pizzolo, Francesco Cavazzini, Elena Saccenti, Gian Matteo Rigolin, Rigolin, Gm, Cibien, F, Martinelli, S, Formigaro, L, Rizzotto, L, Tammiso, E, Saccenti, E, Bardi, A, Cavazzini, F, Ciccone, M, Nichele, I, Pizzolo, G, Zaja, Francesco, Fanin, Renato, Galieni, P, Dalsass, A, Mestichelli, F, Testa, N, Negrini, M, and Cuneo, A.
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Chronic lymphocytic leukemia ,Immunology ,Karyotype ,Oligonucleotides ,Biology ,Biochemistry ,cytogenetics ,FISH ,CLL ,karyotyping ,Cohort Studies ,medicine ,Humans ,Metaphase ,Survival analysis ,In Situ Hybridization, Fluorescence ,Aged ,Aged, 80 and over ,Chromosome Aberrations ,medicine.diagnostic_test ,Chromosome ,Cell Biology ,Hematology ,Middle Aged ,medicine.disease ,Prognosis ,Leukemia, Lymphocytic, Chronic, B-Cell ,Survival Analysis ,Leukemia ,Karyotyping ,Chromosome abnormality ,Interleukin-2 ,Female ,Mitogens ,Fluorescence in situ hybridization - Abstract
It is unclear whether karyotype aberrations that occur in regions uncovered by the standard fluorescence in situ hybridization (FISH) panel have prognostic relevance in chronic lymphocytic leukemia (CLL). We evaluated the significance of karyotypic aberrations in a learning cohort (LC; n = 64) and a validation cohort (VC; n = 84) of patients with chronic lymphocytic leukemia with “normal” FISH. An abnormal karyotype was found in 21.5% and 35.7% of cases in the LC and VC, respectively, and was associated with a lower immunophenotypic score (P = .030 in the LC, P = .035 in the VC), advanced stage (P = .040 in the VC), and need for treatment (P = .002 in the LC, P = < .0001 in the VC). The abnormal karyotype correlated with shorter time to first treatment and shorter survival in both the LC and the VC, representing the strongest prognostic parameter. In patients with chronic lymphocytic leukemia with normal FISH, karyotypic aberrations by conventional cytogenetics with novel mitogens identify a subset of cases with adverse prognostic features.
- Published
- 2012