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Disc-electrophoretic investigation of proteases of the gastric juice of dogs under normal conditions and in experimental atophan ulcer of the stomach demonstrated that in fasting dogs ulceration was accompanied by marked quantitative changes in the protease spectrum. Fraction No. 6 showed a significant elevation against the background of total reduction of proteolytic fraction of the gastric juice in sham feeding of ulcerated dogs. Sham feeding replete ulcerated dogs indicated a marked increase of fractions No.1 and No.2. The noted changes of the gastric juice protease spectrum may be of ulcerogenic significance.

A comparative analysis was made of the aggressive properties of gastric juice secreted by normal people and those suffering from peptic ulcer in response to histamine and pentagastrin. Ulcerogenic activity of gastric juice obtained in response to histamine was shown to increase in peptic ulcer. The "pentagastrin" juice did not exhibit such an effect. Experimental study of the occurrence of gastric juice proteases in dogs revealed an increased occurrence in most proteases and their elevated number of the "histamine" juice in ulcer development and a decreased occurrence of proteases in the "pentagastrin" juice. Low aggressiveness of gastric juice from peptic ulcer patients during pentagastrin-induced stimulation of secretion appears to be related to the listed changes in the spectrum and occurrence of proteases.

In 20 healthy patients the cholesterol esterase activity in duodenal content was examined. The cholesterol ether of o-coumaric acid was used as a substrate. Increase of the cholesterol esterase activity was noted after stimulation of pancreozymin and secretin. The cholesterol esterase concentration in duodenal content changes in more wide range than the index of the output. The cholesterol esterase output is the most significant informative index. There is not any cholesterol esterase activity in bile, gastric juice and saliva. The results obtained have shown that the main part of the estimated cholesterol esterase activity has a pancreatic base. The investigation of the cholesterol esterase activity in duodenal content may be used in the study of the exocrine function of pancreas.

Chronic experiments on dogs have shown that the damage of extra-secretory pancreatic function by duct ligation caused marked compensatory changes of stomach function. The increase in gastric juice secretion and gastric juice proteolytic activity was accompanied by the reduction in its acidity. In addition to quantitative changes, qualitative shifts were revealed (amylolytic activity in strongly acid pH-reaction), never observed in the gastric juice of intact animals. Partial pancreas resection (up to 75%) both in control and test animals 10-14 months after pancreatic duct ligation was not accompanied by significant changes in gastric juice secretion. Total pancreas resection in dogs with previous pancreatic duct ligation caused neither prompt animal death, as in the control, nor the inhibition of compensatory reactions of gastric juice secretion.