1. PCI-24781 can improve in vitro and in vivo developmental capacity of pig somatic cell nuclear transfer embryos.
- Author
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Jin, Long, Zhu, Hai-Ying, Guo, Qing, Li, Xiao-Chen, Zhang, Yu-Chen, Zhang, Guang-Lei, Xing, Xiao-Xu, Xuan, Mei-Fu, Luo, Qi-Rong, Yin, Xi-Jun, and Kang, Jin-Dan
- Subjects
SOMATIC cell nuclear transfer ,BLASTOCYST ,ACETYLATION ,LYSINE ,LABORATORY swine - Abstract
Objective: To examine the effect of PCI-24781 (abexinostat) on the blastocyst formation rate in pig somatic cell nuclear transferred (SCNT) embryos and acetylation levels of the histone H3 lysine 9 and histone H4 lysine 12. Results: Treatment with 0.5 nM PCI-24781 for 6 h significantly improved the development of cloned embryos, in comparison to the control group (25.3 vs. 10.5 %, P < 0.05). Furthermore, PCI-24781 treatment led to elevated acetylation of H3K9 and H4K12. TUNEL assay and Hoechst 33342 staining revealed that the percentage of apoptotic cells in blastocysts was significantly lower in PCI-24781-treated SCNT embryos than in untreated embryos. Also, PCI-24781-treated embryos were transferred into three surrogate sows, one of whom became pregnant and two fetuses developed. Conclusion: PCI-24781 improves nuclear reprogramming and the developmental potential of pig SCNT embryos. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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