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Start Over Author "Na Young Kang" Journal biotechnology letters
3 results on '"Na Young Kang"'

1. [Untitled]

Author

Jong-Eon Chin, Suhn-Young Im, Hwanghee Blaise Lee, Na-Young Kang, Jeong-Nam Park, and Suk Bai

Subjects
Genetics, chemistry.chemical_classification, biology, Strain (chemistry), Saccharomyces cerevisiae, Bioengineering, Promoter, General Medicine, biology.organism_classification, Applied Microbiology and Biotechnology, Yeast, Enzyme, Biochemistry, chemistry, biology.protein, Amylase, Gene, Biotechnology, Alcohol dehydrogenase
Abstract

The gene encoding Schwanniomyces occidentalis α-amylase (AMY) was introduced into the chromosomal δ sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an δ-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AMY gene and aureobasidin A resistance gene (AUR1-C) as the selection marker. The AMY gene was expressed under the control of the alcohol dehydrogenase gene promoter (ADC1p). The α-amylase activity of Sacc. cerevisiae transformed with this integrative cassette was 6 times higher than that of Sch. occidentalis. The transformants (integrants) were mitotically stable after 100 generations in nonselective medium.

Published
2003
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2. Construction of an amylolytic industrial strain of Saccharomyces cerevisiae containing the Schwanniomyces occidentalis alpha-amylase gene

Author

Na-Young, Kang, Jeong-Nam, Park, Jong-Eon, Chin, Hwanghee Blaise, Lee, Suhn-Young, Im, and Suk, Bai

Subjects
Enzyme Activation, Industrial Microbiology, Gene Expression Regulation, Fungal, Saccharomycetales, Saccharomyces cerevisiae, alpha-Amylases, Protein Engineering, Gene Expression Regulation, Enzymologic, Recombinant Proteins
Abstract

The gene encoding Schwanniomyces occidentalis alpha-amylase (AMY) was introduced into the chromosomal delta sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an delta-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AMY gene and aureobasidin A resistance gene (AUR1-C) as the selection marker. The AMY gene was expressed under the control of the alcohol dehydrogenase gene promoter (ADC1p). The alpha-amylase activity of Sacc. cerevisiae transformed with this integrative cassette was 6 times higher than that of Sch. occidentalis. The transformants (integrants) were mitotically stable after 100 generations in nonselective medium.

Published
2003

3. Construction of an amylolytic industrial strain of Saccharomyces cerevisiae containing the Schwanniomyces occidentalis α-amylase gene.

Author

Na-Young Kang, Jeong-Nam Park, Jong-Eon Chin, Hwanghee Blaise Lee, Suhn-Young Im, and Suk Bai

Subjects
SACCHAROMYCES cerevisiae, ALCOHOL, DEHYDROGENASES, NUCLEIC acids, NUCLEOTIDE sequence, GENETIC code
Abstract

The gene encoding Schwanniomyces occidentalis α-amylase (AMY) was introduced into the chromosomal δ sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an δ-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AMY gene and aureobasidin A resistance gene (AUR1-C) as the selection marker. The AMY gene was expressed under the control of the alcohol dehydrogenase gene promoter (ADC1p). The α-amylase activity of Sacc. cerevisiae transformed with this integrative cassette was 6 times higher than that of Sch. occidentalis. The transformants (integrants) were mitotically stable after 100 generations in nonselective medium. [ABSTRACT FROM AUTHOR]

Published
2003
Full Text
View/download PDF

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