1. Differential miRNA expression analysis of extracellular vesicles from brain microvascular pericytes in spontaneous hypertensive rats
- Author
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Jinna Yang, Honggang Zhang, Bingwei Li, Ruijuan Xiu, Qingbin Wu, Xiaochen Yuan, and Ruiqin Han
- Subjects
0106 biological sciences ,0301 basic medicine ,Bioengineering ,01 natural sciences ,Applied Microbiology and Biotechnology ,Rats, Inbred WKY ,Pathogenesis ,03 medical and health sciences ,Extracellular Vesicles ,Downregulation and upregulation ,010608 biotechnology ,Rats, Inbred SHR ,microRNA ,Animals ,Nucleotide ,Circulating MicroRNA ,KEGG ,Gene ,chemistry.chemical_classification ,Brain ,General Medicine ,Extracellular vesicle ,Cell biology ,Rats ,030104 developmental biology ,chemistry ,Gene Expression Regulation ,Hypertension ,Microvessels ,Signal transduction ,Pericytes ,Biotechnology - Abstract
This study is to explore the exact roles of extracellular vesicle (EVs) miRNAs from brain microvascular pericytes in the pathogenesis of hypertension. Forty-eight significantly differentially expressed miRNAs were identified, of which 17 were found to be upregulated and 31 were found to be downregulated in brain microvascular pericytes of spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The GO enrichment analysis verified that the target genes were enriched in signaling pathways and molecular functions, such as metal ion binding, nucleotide binding and ATP binding. The KEGG analysis indicated that the target genes were enriched in Linoleic acid, alpha-linolenic acid and sphingolipid metabolism pathways. Several EV derived miRNAs, such as miR-21-5p, let-7c-5p and let-7a-5p, were found to be abnormally expressed in brain microvascular pericytes obtained from spontaneous hypertensive rats, compared with that of normotension Wistar Kyoto rats. The results of our research provide more insights into the functional link between brain microvascular pericytes and the pathogenesis of hypertension.
- Published
- 2019