1. Production of cellulosic organic acids via synthetic fungal consortia.
- Author
-
Scholz SA, Graves I, Minty JJ, and Lin XN
- Subjects
- Cellulose metabolism, Coculture Techniques, Fermentation, Glucans metabolism, Glucose metabolism, Rhizopus growth & development, Trichoderma growth & development, Xylans metabolism, Zea mays metabolism, Fumarates metabolism, Lactic Acid metabolism, Microbial Consortia physiology, Rhizopus physiology, Trichoderma physiology
- Abstract
Consolidated bioprocessing (CBP) is a potential breakthrough technology for reducing costs of biochemical production from lignocellulosic biomass. Production of cellulase enzymes, saccharification of lignocellulose, and conversion of the resulting sugars into a chemical of interest occur simultaneously within a single bioreactor. In this study, synthetic fungal consortia composed of the cellulolytic fungus Trichoderma reesei and the production specialist Rhizopus delemar demonstrated conversion of microcrystalline cellulose (MCC) and alkaline pre-treated corn stover (CS) to fumaric acid in a fully consolidated manner without addition of cellulase enzymes or expensive supplements such as yeast extract. A titer of 6.87 g/L of fumaric acid, representing 0.17 w/w yield, were produced from 40 g/L MCC with a productivity of 31.8 mg/L/hr. In addition, lactic acid was produced from MCC using a fungal consortium with Rhizopus oryzae as the production specialist. These results are proof-of-concept demonstration of engineering synthetic microbial consortia for CBP production of naturally occurring biomolecules., (© 2017 Wiley Periodicals, Inc.)
- Published
- 2018
- Full Text
- View/download PDF