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The coronavirus pandemic affects the lifestyle, mental and physical health of people, especially those at direct risk of becoming infected with SARS-CoV-2. In the period from November 2020 to February 2021, data were collected from 296 healthcare workers through the GAD-7 generalized anxiety questionnaire, as well as through self-assessment of their physical and mental health status, and the degree of perceiving the coronavirus as a threat. The results show that about one-third of the participating healthcare workers experienced mild generalized anxiety, and about one-fifth of them, moderate or severe symptoms of anxiety. Health professionals with the highest generalized anxiety were younger, with deteriorated physical and mental health (not only subjectively estimated by the participants but also objectively diagnosed health workers with coronavirus), less religious, having an intimate partner. More perceived threat of coronavirus was associated with increased generalized anxiety in healthcare professionals. Healthcare workers with a higher level of generalized anxiety were more likely to have difficulties doing their jobs, taking care of things at home, or getting along with other people. The anxiety experienced is of immense importance for the functioning of the individual. It is recommendable for healthcare specialists to feel that they receive social support to reduce their anxiety levels and to better balance their work and personal life, having more free time to take care of themselves and their close relationships.