1. Single-molecule fluorescence observed with mercury lamp illumination.
- Author
-
Unger M, Kartalov E, Chiu CS, Lester HA, and Quake SR
- Subjects
- Green Fluorescent Proteins, Image Processing, Computer-Assisted, Light, Luminescent Proteins ultrastructure, Microscopy, Fluorescence instrumentation, Peptides chemistry, Plasmids genetics, Rhodamines chemistry, Streptavidin ultrastructure, Histidine, Mercury, Microscopy, Fluorescence methods
- Abstract
We demonstrate that it is possible to observe single fluorescent molecules using a standard fluorescence microscope with mercury lamp excitation and an inexpensive cooled charge-coupled device (CCD) camera. With this equipment, we have been able to observe single molecules of tetramethyl-rhodamine, rhodamine 6G, fluorescein isothiocyanate and green fluorescent protein. Immobilized molecules were observed both in air and in aqueous solution.
- Published
- 1999
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