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Start Over Author "Bocanegra J" Journal biotechniques
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3. Expression of the highly toxic centromere binding protein CENP-B in E. coli using the pET system in the absence of the inducer IPTG.

Author

Bocanegra JA, Bejarano LA, and Valdivia MM

Subjects
Animals, Blotting, Western, Centromere Protein B, Cricetinae, Deoxyribonuclease BamHI metabolism, Deoxyribonucleases, Type II Site-Specific metabolism, Electrophoresis, Polyacrylamide Gel, Isopropyl Thiogalactoside pharmacology, Polymerase Chain Reaction, Autoantigens, Centromere metabolism, Chromosomal Proteins, Non-Histone genetics, DNA-Binding Proteins, Escherichia coli genetics, Gene Expression
Published
1997
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