1. A multi-channel electroporation microchip for gene transfection in mammalian cells
- Author
-
Chanil Chung, Neon-Cheol Jung, Young Shik Shin, Jun Keun Chang, Jeong Ah Kim, and Keunchang Cho
- Subjects
Materials science ,Green Fluorescent Proteins ,Biomedical Engineering ,Biophysics ,Nanotechnology ,CHO Cells ,Transfection ,chemistry.chemical_compound ,Cricetulus ,Cricetinae ,Lab-On-A-Chip Devices ,Electric field ,Electrochemistry ,Animals ,Humans ,Multi channel ,Polydimethylsiloxane ,Electroporation ,Chinese hamster ovary cell ,General Medicine ,Chip ,chemistry ,Plasmids ,Biotechnology ,Voltage - Abstract
We developed a multi-channel electroporation microchip made of polydimethylsiloxane (PDMS) and glass for gene transfer in mammalian cells. This chip produces multiple electric field gradients in a single microchip by varying the lengths of the microchannels from 2 to 4 cm. Electric fields of 0.65, 0.57, 0.49, 0.41, and 0.33 kV/cm were simultaneously produced in a single chip when the voltage of 1.3 kV was applied. We transferred enhanced green fluorescent protein genes (pEGFP) into HEK-293 and CHO cells, which were cultured within the microchannels. The feasibility of our device was demonstrated because it was able to produce five different transfection rates and survival rates at different electric fields produced in a single microchip. This system is expected to optimize the experimental conditions in gene transfection research more easily and faster than conventional electroporation methods.
- Published
- 2007