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Your search keyword '"Moscone D"' showing total 14 results
Start Over Author "Moscone D" Journal biosensors and bioelectronics
14 results on '"Moscone D"'

14. Novel bio-lab-on-a-tip for electrochemical glucose sensing in commercial beverages

Author

Danila Moscone, Fabiana Arduini, Vincenzo Mazzaracchio, Stefano Cinti, Roberta Marrone, Cinti, S., Marrone, R., Mazzaracchio, V., Moscone, D., and Arduini, F.

Subjects
Materials science, Biomedical Engineering, Biophysics, Lab-on-a-tip, Nanotechnology, Biosensing Techniques, 02 engineering and technology, 01 natural sciences, Beverages, Matrix (chemical analysis), Glucose Oxidase, Electroanalysi, Settore CHIM/01, Electrochemistry, Miniaturization, Glucose oxidase, Beverage, Electrodes, Detection limit, biology, 010401 analytical chemistry, Pipette, Electrochemical Techniques, General Medicine, Chronoamperometry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Glucose, Filter (video), biology.protein, 0210 nano-technology, Biosensor, Biotechnology
Abstract

The development of portable and user-friendly sensing platforms is a hot topic in the field of analytical chemistry. Among others, electroanalytical approaches exhibit a high amenability for reaching this purpose, i.e. the commercial strips for diabetes care are an obvious success. However, providing fully-integrated and reagent-free methods is always a leitmotiv. In this work, we evaluated the use of a disposable pipette tip, opportunely configured to demonstrate the first example of an electrochemical biosystem in a pipette tip, namely bio-lab-on-a-tip. The combination of a pipette tip, wire electrodes, enzyme, and cotton wool filter, allows the fabrication of a novel electroanalytical platform that does not need expertise-required tasks. To demonstrate the feasibility of this novel method, glucose is detected in beverages by means of chronoamperometry. The experimental setup, entirely built inside the pipette tip, is able to 1) block impurities/interferences from matrix, 2) load/release reagents for the bio-assay, 3) reduce the operating task to zero, and 4) perform electrochemical detection. With optimized experimental parameters, the bio-lab-on-a-tip is able to detect glucose linearly up to 10 mM with a detection limit of 170 μM. The effectiveness of the platform was confirmed by testing commercial beverages, e.g. Coca-Cola and Coca-Cola Zero, with high accuracy. In addition, the shelf-life of the novel device was evaluated, highlighting the role of cotton wool filter for providing a suitable environment for glucose oxidase stability. The novel concept can be easily generalized for further applications in the field of non-invasive clinical diagnostics and in-situ environmental monitoring.

Published
2020

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