1. Portable amperometric immunosensor for histamine detection using Prussian blue-chitosan-gold nanoparticle nanocomposite films.
- Author
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Dong, Xiu-Xiu, Yang, Jin-Yi, Luo, Lin, Zhang, Yi-Feng, Mao, Chuanbin, Sun, Yuan-Ming, Lei, Hong-Tao, Shen, Yu-Dong, Beier, Ross C., and Xu, Zhen-Lin
- Subjects
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HISTAMINE , *BIOSENSORS , *IMMUNE system , *NANOPARTICLES , *NANOCOMPOSITE materials - Abstract
Histamine (HA) is a biogenic amine that can accumulate to high concentration levels in food as a result of microbial activity and can cause toxic effects in consumers. In this work, a portable electrochemical immunosensor capable of detecting HA with high sensitivity and selectivity was developed. Prussian blue-chitosan-gold nanoparticle (PB-CS-AuNP) nanocomposite films with excellent biocompatibility were synthesized and characterized by scanning electron microscopy and energy dispersive X-ray analysis. The PB-CS-AuNP were coated onto a screen-printed electrode by one-step electrodeposition and used to conjugate the HA ovalbumin conjugate (HA-Ag). HA was determined by a competition between the coating HA-Ag and the HRP labeled HA antibody (HRP-HA-Ab). After careful optimization of assay conditions and Box-Behnken analysis, the developed immunosensor showed a linear range from 0.01 to 100 μg/mL for HA in fish samples. The average recoveries from spiked samples ranged from 97.25% to 105%. The biosensor also showed good specificity, reproducibility, and stability, indicating its potential application in monitoring HA in a simple and low cost manner. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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