1. Sensitive reagentless electrochemical immunosensor based on an ormosil sol–gel membrane for human chorionic gonadotrophin
- Author
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Tan, Fang, Yan, Feng, and Ju, Huangxian
- Subjects
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GONADOTROPIN , *ENZYMES , *METALLOENZYMES , *BLOOD plasma - Abstract
Abstract: A new organically modified silicate (ormosil) material was synthesized as a matrix to encapsulate enzyme labeled antibody for preparation of immunosensors. The ormosil matrix was prepared by hydrolyzing tetraethyoxysilane and (3-aminopropyl)triethoxysilane in weak alkali solution. It possessed three-dimensional ordered nanoporous structure with high electrical conductivity and good mechanical stability. Its hydrophilicity provided a microenvironment for retaining the biological activity of the immobilized protein. Particularly, using horseradish peroxidase-labeled human chorionic gonadotrophin antibody (HRP-anti-hCG) as a model, the immobilized HRP showed direct electron transfer at about −35mV with a rate constant of 15.8±3.8s−1. By a simple one-step immunoreaction between human serum chorionic gonadotrophin (hCG) in sample solution and the immobilized HRP-anti-hCG, the differential pulse voltammetric peak current of HRP decreased linearly with an increasing hCG concentration from 0.5 to 50mIU/ml with a relatively low limit of detection of 0.3mIU/ml at 3σ. Excellent analytical performance, fabrication reproducibility and operational stability of the proposed biosensor indicated its promising application in clinical diagnostics. [Copyright &y& Elsevier]
- Published
- 2007
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