1. Proximity labeling-assisted click conjugation for electrochemical analysis of specific subpopulations in circulating extracellular vesicles.
- Author
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Cao, Yue, Zhou, Liang, Zhou, Guozhang, Liu, Wensheng, Cui, Haiyan, Cao, Ya, Zuo, Xiaolei, and Zhao, Jing
- Subjects
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EXTRACELLULAR vesicles , *ELECTROCHEMICAL analysis , *EPIDERMAL growth factor , *SILVER nanoparticles , *CLICK chemistry , *EPIDERMAL growth factor receptors , *CANCER diagnosis - Abstract
Sensitive and accurate analysis of specific subpopulations in circulating extracellular vesicles (EVs) can provide a wealth of information for cancer diagnosis and management. Thus, we propose herein a new electrochemical biosensing method based on a proximity labeling-assisted click conjugation strategy. The method's core design is use of antibody-guided proximity labeling to equip target EVs with a large amount of alkyne groups, so that azide-tagged silver nanoparticles can be accurately loaded onto target EV surfaces, via click conjugation, to generate significant electrochemical responses. Adopting CD44-positive EVs as the model, the electrochemical method was demonstrated by analyzing target EVs across a wide linear range (103–109 particles/mL) with acceptable sensitivity and specificity. Satisfactory utility in clinical blood samples, and versatility with human epidermal growth factor receptor-2-positive EVs as alternative targets, were also shown. This method may thus provide a novel approach to specific subgroup analyses of circulating EVs, and is expected to offer reliable guidance for cancer diagnoses and management strategies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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