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The article reports on the results of a study of the coexistence of Ser84 in renin and His13 in angiotensinogen which brings a pH profile of two separate peaks to the reaction of human renin and sheep angiotensionogen. A description of the experimental set-up and measurement methods is presented. The study indicated that sheep angiotensinogen has a factor other than His13 to enhance the activity of human renin at basic pH.

The article investigates the pH dependence of the reaction of various renins using sheep angiotensinogen as a substrate. The pH profile of the human renin reaction showed two separate peaks against sheep angiotensinogen. The article indicates that Ser84 of human renin contributes to the biphasic pH dependence of the renin-angiotensinogen reaction.

The article focuses on the production of human prorenin attached by a decahistidine tag at the C-terminus in Chinese hamster ovary cells. The tagged protein secreted into the culture medium was activated to mature renin by proteolytic removal of its prosegment by trypsin in the same manners a native prorenin. The tagged protein was also in the inactive prorenin form.

Examines the role of angiotensins II and III in regulating water flow through aquaporins in clam worms of Perinereis. Treatment of the clam worms with tetrachloroaurate (III) before and after angiotensin treatment; Function of tetrachloroaurate as blocker of aquaporins; Role of the angiotensins in increasing body weight in clam worms under a hypo-osmotic condition and decreasing body weight under a hyper-osmotic condition.