Search

Your search keyword '"Cristiane Uchima"' showing total 1 results
Start Over Author "Cristiane Uchima" Journal bioscience, biotechnology, and biochemistry
1 results on '"Cristiane Uchima"'

1. Expression and one-step purification of recombinant proteins using an alternative episomal vector for the expression of N-tagged heterologous proteins in Pichia pastoris

Author

Manabu Arioka and Cristiane Uchima

Subjects
Blotting, Western, Genetic Vectors, Heterologous, Isoptera, Applied Microbiology and Biotechnology, Biochemistry, Chromatography, Affinity, Pichia, Analytical Chemistry, Pichia pastoris, law.invention, Proto-Oncogene Proteins c-myc, Plasmid, Western blot, law, medicine, Methods, Animals, Cellulases, Histidine, Molecular Biology, biology, medicine.diagnostic_test, Chemistry, Organic Chemistry, General Medicine, biology.organism_classification, Molecular biology, Recombinant Proteins, Recombinant DNA, Heterologous expression, Oligopeptides, Biotechnology, Plasmids
Abstract

Here we report the construction of an alternative episomal vector, pBGP3, which allows the expression of heterologous proteins with N-terminal hexahistidine and myc-epitope tags in Pichia pastoris. To test the usefulness of pBGP3, four cellulases from termites were expressed. Production was confirmed by activity assays and Western blot using anti-c-Myc antibody. Purification was performed by single-step Ni(2+)-affinity chromatography, which confirmed the efficiency of pBGP3.

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results