1. Glu–Phe from onion (Allium Cepa L.) attenuates lipogenesis in hepatocytes
- Author
-
Yu Geon Lee, Tae–Il Jeon, Cho Jeong Yong, Eom Ji Hwang, and Jae–Hak Moon
- Subjects
0301 basic medicine ,Cell Survival ,Chemical structure ,AMP-Activated Protein Kinases ,Applied Microbiology and Biotechnology ,Biochemistry ,Cell Line ,Analytical Chemistry ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Onions ,Animals ,Protein kinase A ,Molecular Biology ,Gene ,Hypolipidemic Agents ,biology ,Plant Extracts ,Lipogenesis ,Organic Chemistry ,food and beverages ,AMPK ,Dipeptides ,General Medicine ,biology.organism_classification ,Sterol ,Fatty Acid Synthase, Type I ,030104 developmental biology ,Gene Expression Regulation ,Hepatocytes ,Allium ,lipids (amino acids, peptides, and proteins) ,Sterol Regulatory Element Binding Protein 1 ,Stearoyl-CoA Desaturase ,030217 neurology & neurosurgery ,Homeostasis ,Acetyl-CoA Carboxylase ,Signal Transduction ,Biotechnology - Abstract
A Glu–Phe (EF) was isolated from onion (Allium cepa L. cv. Sunpower). The chemical structure of EF was determined by nuclear magnetic resonance and electrospray ionization–mass (ESI−MS) spectroscopy. We showed that EF reduced lipid accumulation in mouse hepatocytes by inhibiting the expression of sterol regulatory element-binding protein-1c (SREBP–1c) and its lipogenic target genes. We also found that AMP-activated protein kinase (AMPK) was required for the inhibitory effect of EF on lipid accumulation in mouse hepatocytes. Furthermore, EF was qualified in nine onion cultivars by selective multiple reaction-monitoring detection of liquid chromatography–ESI−MS. These results suggest that EF could contribute to the beneficial effect of onion supplement in maintaining hepatic lipid homeostasis.
- Published
- 2017