Search

The solid-state fermentation (SSF) of ammoniated corn straw (ACS) by Pleurotus ostreatus Pl-5 was investigated. The SSF experiments were carried out for 20 d using ACS and corn straw (CS) as the substrates of the experimental group (EP) and control group (CP), respectively. The effects of the ammoniation pretreatment on the CS lignocellulose structure, fungal growth, enzyme production, and components of CS during the SSF process were analyzed. The ammoniation pretreatment effectively degraded the lignin and hemicellulose contents in the CS, by 15.3% and 7.7%, respectively. Thus, the in vitro digestibility (IVD) of the EP was higher than for the CP, and even higher than the ligninase activities (laccase: 661 U/g; MnP: 56.8 U/g) found in the CP. The higher cellulase activities (CMCase: 152.3 U/g; FPA: 224.7 U/g) in the EP improved the cellulose degradation, which also promoted the P. ostreatus Pl-5 growth, and the high total N content significantly increased the EP fungal biomass and amino acid contents. A shorter processing time and a higher level of nutrients were achieved by the SSF of ACS, which showed its potential for use in animal feed production.

This study investigated the effect of ammoniation pretreatment of corn stalk (CS) with different temperatures, ammonia proportions, and processing times on lignocellulolytic enzyme production in solid-state fermentation (SSF) by Lentinus edodes L-8. The total N content and lignocellulose contents of ammoniated corn stalk were determined for analysis of the effect of ammoniation pretreatment on lignocellulose structure. The variation patterns of enzyme activity were analyzed according to the enzyme data determined every 2 days during the fermentation. A 4% w/w high-temperature ammoniation pretreatment had a significant effect on cellulase production, and the highest enzyme activity reached almost triple that of the control group. The results also showed that ammoniation pretreatment inhibited the generation of ligninases, such that ligninases appeared later and at lower activities in experimental groups compared to the control group.