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[Display omitted] • ML-based model optimized operation parameters of immobilized microalgae for growth. • Continuous illumination achieved AW recycling and improved microalgal reutilization. • Symbiotic bacteria enhanced C&N assimilation of microalgae for lipid/starch synthesis. Immobilized microalgae biotechnologies can conserve water and space by low-carbon wastewater treatment and resource recovery in a recirculating aquaculture system (RAS). However, technical process parameters have been unoptimized considering the mutual interaction between factors. In this study, machine learning optimized the parameters of alginate-immobilized Chlorella vulgaris (C. vulgaris), that is, 474 μmol/(m2·s) of light intensity, 23 × 106 cells/mL for initial cell number, and 2.07 mm particle size. Importantly, under continuous illumination, the immobilized C. vulgaris and microalgal-bacterial consortium improved water purification and biomass reutilization. Transcriptomics of C. vulgaris showed enhanced nitrogen removal by increasing pyridine nucleotide and lipid accumulation via enhanced triacylglycerol synthesis. Symbiotic bacteria upregulated genes for nitrate reduction and organic matter degradation, which stimulated biomass accumulation through CO 2 fixation and starch synthesis. The recoverable microalgae (1.94 g/L biomass, 47 % protein, 26.23 % lipids), struvite (64.79 % phosphorus), and alginate (79.52 %) every two weeks demonstrates a low-carbon resource recovery in RAS. [ABSTRACT FROM AUTHOR]

[Display omitted] • Novel integrated recirculating aquaculture systems (RAS) using microalgae is suggested. • Microalgae decarbonate RAS by mitigating energy-demanding water treatment processes. • Microalgae provide O 2 and sequester CO 2 , boosting RAS efficiency and sustainability. • Phycoremediation mitigates the growth-inhibiting factors of fish in RAS. • Coupled hydrothermal liquefaction of fecal waste provides bioavailable nutrients. In recirculating aquaculture systems (RAS), waste management of nutrient-rich byproducts accounts for 30–50% of the whole production costs. Integrating microalgae into RAS offers complementary solutions for transforming waste streams into valuable co-products. This review aims to provide an overview of recent advances in microalgae application to enhance RAS performance and derive value from all waste streams by using RAS effluents as microalgal nutrient sources. Aquaculture solid waste can be converted by hydrothermal liquefaction (HTL), then the resultant aqueous phase of HTL can be used for microalgae cultivation. In addition, microalgae generate the required oxygen while sequestering carbon dioxide. The review suggests a novel integrated system focusing on oxygenation and carbon dioxide capture along with recent technological developments concerning efficient microalgae cultivation and nutrient recovery techniques. In such system, microalgae-based biorefineries provide environmentally-conscious and economically-viable pathways for enhanced RAS performance and conversion of effluents into high-value products. [ABSTRACT FROM AUTHOR]

[Display omitted] • Synthesis methods and removal mechanisms of pristine BC for phosphate are presented. • Various methods of synthetic metal-doped BC for enhancing adsorption are proposed. • Critical factors influencing adsorption performance of metal-doped BC are analyzed. • Mechanisms of metal-doped BC for selective phosphate adsorption are summarized. • Constructive suggestions for further applications of metal-doped BC are put forward. Phosphorus (P) plays a crucial role in plant growth, which can provide nutrients for plants. Nonetheless, excessive phosphate can cause eutrophication of water, deterioration of aquatic environment, and even harm for human health. Therefore, adopting feasible adsorption technology to remove phosphate from water is necessary. Biochar (BC) has received wide attention for its low cost and environment-friendly properties. However, undeveloped pore structure and limited surface groups of primary BC result in poor uptake performance. Consequently, this work introduced the synthesis of pristine BC, parameters influencing phosphate removal, and corresponding mechanisms. Moreover, multifarious metal-doped BCs were summarized with related design principles. Meanwhile, mechanisms of selective phosphate adsorption by metal-doped BC were investigated deeply, and the recovery of phosphate from water, and the utilization of phosphate-loaded adsorbents in soil were critically presented. Finally, challenges and prospects for widespread applications of selective phosphate adsorption were proposed in the future. [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel QQ fungus Vanrija sp. MS1 was isolated from activated sludge. • MS1 lowers the biofouling rate and EPS production in membrane bioreactor. • MS1 causes a shift in microbe populations linked to biofouling. • MS1 has greater potency under acidic conditions than Rhodococcus sp. BH4. This study addresses membrane biofouling in membrane bioreactors (MBRs) by exploring fungal-to-bacterial quorum quenching (QQ) strategies. While most research has been focused on bacterial-to-bacterial QQ tactics, this study identified fungal strain Vanrija sp. MS1, which is capable of degrading N -acyl-homoserine lactones (signaling molecules of Gram-negative bacteria). To determine the benefits of fungal over bacterial strains, after immobilization on fluidizing spherical beads in an MBR, MS1 significantly reduced the fouling rate by 1.8-fold compared to control MBR, decreased extracellular polymeric substance levels in the biofilm during MBR operation, and favorably changed microbial community and bacterial network, resulting in biofouling mitigation. It is noteworthy that, unlike Rhodococcus sp. BH4, MS1 enhanced QQ activity when switching from neutral to acidic conditions. These results suggest that MS1 has the potential for the effective treatment of acidic industrial wastewater sources such as semiconductor and secondary battery wastewater using MBRs. [ABSTRACT FROM AUTHOR]

[Display omitted] • Embedding baffles in CW-MFC increased water flowing path and contact opportunity. • Combined secondary and tertiary water treatment was achieved in baffled flow CW-MFC. • Baffled flow CW-MFC had higher ammonium and total nitrogen removal efficiency. • Embedding 1 or 2 baffles in the cathode chamber led to higher maximum power density. • CW-MFC with baffles embedded in the cathode unit had different microbial community. Baffled flow constructed wetland-microbial fuel cell (BFCW-MFC) coupling systems were constructed with baffles embedded in cathode chamber. The performance of BFCW-MFCs operated at different hydraulic retention times (HRTs) was evaluated. At the representative HRT of 48 h, embedding 1 or 2 baffles (i.e., BFCW-MFC1 and BFCW-MFC2) produced 32.9 % (29.5 mW/m3) and 53.2 % (34.0 mW/m3) more power density than control system (22.2 mW/m3), respectively. Comparable organics biodegradation efficiencies were observed in BFCW-MFCs at the same HRTs. BFCW-MFC1 and BFCW-MFC2 had higher ammonium and total nitrogen removal efficiency. All systems had decreased nitrogen removal performance as shortening HRT from 72 to 12 h. Multiple nitrogen removal processes were involved, including ammonium oxidation, anammox, and heterotrophic and autotrophic denitrification. The predominant bacteria on electrodes were identified for analyzing bioelectricity generation and wastewater treatment processes. Generally, simultaneous wastewater treatment and bioelectricity generation were obtained in BFCW-MFCs, and embedding 1 or 2 baffles was preferable. [ABSTRACT FROM AUTHOR]

• The EL model performs best when the dataset is amplified by a factor of two. • The XGBoost model shows the best performance for predicting effluent COD and TEC. • The XGBoost-NSGA-II model reduces effluent COD by 15% and TEC by 18%. [Display omitted] Pollution integration and carbon reduction has become a primary focus in wastewater treatment processes. In this study, water quality and control indicators were used as input features and the dataset was extended using the moving average method. Random Forest, eXtreme Gradient Boosting (XGBoost), and Light Gradient Boosting Machine algorithms were used to predict the effluent chemical oxygen demand (COD) and total energy consumption (TEC). The results indicated that the model prediction performance could be effectively improved when the data were amplified by two times and that the XGBoost model exhibited the best prediction performance for effluent COD and TEC. The Non-dominated Sorting Genetic Algorithm II model was employed for the multi-objective optimization of effluent COD and TEC, resulting in reductions of 15% and 18%, respectively. The ensemble learning model proposed in this study to achieve synergy between water quality improvement and energy saving is practical. [ABSTRACT FROM AUTHOR]

[Display omitted] • BCPC was fabricated by in-situ self-assembly and MOF-immobilized Lac strategies. • The amount of laccase immobilized on aerogel was 198.15 mg/g, showing high activity. • The designed BCPC has excellent use and storage stability and reusability. • The degradation rates of the BCPC for MB and RB reached 100 % and 96.1 % within 10 min. • The degradation rate of BCPC remained above 75% after repeated use for 10 times. To achieve efficient dye degradation, we reported a visible light-driven biomass photo-enzyme coupled system, which was constructed by assembling g-C 3 N 4 during in situ culture and immobilizing laccase via metal–organic framework (MOF). Benefited from the network and porous structure of bacterial cellulose (BC), the g-C 3 N 4 could be stably interspersed, and MOF grew g-C 3 N 4 /BC to encapsulate laccase. BC improves the reusability of the system, while combined with MOF encapsulation, avoiding direct contact between photo- and enzyme- catalysts. Importantly, thanks to the existence of electron transfer from photocatalysis to enzyme, the photogenerated electron hole recombination within the photocatalyst reduced, improving catalyzed reaction efficiency. The degradation efficiency of the catalysis system within 10 min for methylene blue and rhodamine B could reach 100 % and 96.1 %, respectively, which could rapidly degrade dye and recycle for more than 10 times. This research can shine new light on the development of advanced wastewater treatment. [ABSTRACT FROM AUTHOR]

[Display omitted] • Underwater light source obviously affected the microecosystem of V. spinulosa Yan. • Removal loads of TN and TP were enhanced by 61.5 and 8.5 mg m−2 d-1, respectively. • Growth of leaf and root of V. spinulosa Yan were obviously improved. • Abundance of Methylocystis , Dechloromonas was reduced but Clostridium was increased. • Metabolism of carbohydrate, lipid, and energy of V. spinulosa Yan were promoted. Submerged macrophytes are effective in ecological restoration of water bodies polluted by nitrogen and phosphorus, and its restoration capacity depends on underwater illumination condition. This study explored the influencing mechanism of illumination on Vallisneria spinulosa Yan (V. spinulosa Yan) for water restoration. Addition of underwater light source increased the total nitrogen, ammonia nitrogen, total phosphorus, and phosphate removal loads of the V. spinulosa Yan growth system by 61.5, 39.2, 8.5, and 5.0 mg m−2 d-1, respectively. Meanwhile, the growth of V. spinulosa Yan was obviously promoted, even with high water turbidity. Although the biological nitrogen removal processes were inhibited by adding underwater light source, the growth of V. spinulosa Yan can be significantly improved, thus enhancing the efficiency of water purification via the absorption of nitrogen and phosphorus by V. spinulosa Yan. This study provides a theoretical foundation and technical support for application of submerged macrophytes in ecological water restoration. [ABSTRACT FROM AUTHOR]

The two-sludge anoxic dephosphation (DEPHANOX) process frequently encounters the challenge of elevated effluent ammonia levels in practical applications. In this study, the anaerobic ammonium oxidation (anammox) biofilm was introduced into the DEPHANOX system, transforming it into a three-sludge system, enabling synchronous nitrogen and phosphorus elimination, particularly targeting ammonia. Despite a chemical oxygen demand/total nitrogen ratio of 4.3 ± 0.8 in the actual municipal wastewater and 4.5 h of aeration, the effluent total nitrogen was 13.7 mg/L, lower than the parallel wastewater treatment plant. Additionally, the effluent ammonia reduced to 5.1 ± 2.5 mg/L. Notably, denitrifying phosphorus removal and anammox were coupled in the anoxic zone, yielding 74.5 % nitrogen and 87.8 % phosphorus removal. 16S rRNA gene sequencing identified denitrifying phosphorus-accumulating organisms primarily in floc sludge (Saprospiraceae 7.07 %, Anaerolineaceae 1.95 %, Tetrasphaera 1.57 %), while anammox bacteria inhabited the biofilm (Candidatus Brocadia 4.00 %). This study presents a novel process for efficiently treating municipal wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

This study proposed an efficient framework for optimizing the design and operation of combined systems of wastewater treatment plants (WWTP) and constructed wetlands (CW). The framework coupled a WWTP model with a CW model and used a multi-objective evolutionary algorithm to identify trade-offs between energy consumption, effluent quality, and construction cost. Compared to traditional design and management approaches, the framework achieved a 27 % reduction in WWTP energy consumption or a 44 % reduction in CW cost while meeting strict effluent discharge limits for Chinese WWTP. The framework also identified feasible decision variable ranges and demonstrated the impact of different optimization strategies on system performance. Furthermore, the contributions of WWTP and CW in pollutant degradation were analyzed. Overall, the proposed framework offers a highly efficient and cost-effective solution for optimizing the design and operation of a combined WWTP and CW system., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Ltd.)

Sunlight illumination has the potential to control the stability and sustainability of dynamic membrane (DM) systems. In this study, an up-flow anaerobic sludge blanket (UASB) reactor was combined with DM under different illumination positions (direct, indirect and no illumination) to treat wastewater. Results indicated that the UASB achieved a COD removal up to 87.05 % with an average methane production of 0.28 L/d. Following treatment by the UASB, it was found that under illumination, the removal of organic substances by DM exhibited poor performance due to algal proliferation. However, the DM systems demonstrated efficient removal of ammonia nitrogen, ranging from 96.21 % to 97.67 % after stabilization. Total phosphorus removal was 45.72 %, and membrane flux remained stable when directly illuminated. Conversely, the DM system subjected to indirect illumination showed unstable membrane flux and severe fouling resistance. These findings offer valuable insights into optimizing illumination positions in DM systems under anaerobic conditions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Lactic acid-rich fermentation liquid (LAFL) of food waste is found to act as a promising alternative carbon source for nitrogen removal in wastewater treatment. Here, LAFL was employed to investigate its impacts on nitrogen removal during raw municipal wastewater treatment with a comparison to sodium acetate (NaAc). Results indicated that nitrogen removals were comparable when incorporated with LAFL and NaAc (92.89 % v.s. 91.23 %). Unlike the utilization of NaAc, using LAFL could avoid suppressing the relative abundance of the nitrification genes and thus pose a negative risk to nitrogen removal during prolonged operation. The introduction of LAFL increased the stability and robustness of the functional microbial community and effectively reduced excess activated sludge (AS) generation by 109 % compared to NaAc addition, consequently enhancing nitrogen removal but diminishing the treatment cost. In general, LAFL exhibits prospective engineering application potentials and economic advantages in improving nitrogen removal by AS process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

[Display omitted] • Integrated process with subcritical water and xylanolytic enzymes is developed. • Up to 75% of extracted feruloylated arabinoxylan from wheat bran. • Minimal enzyme cocktail with a xylanase and a feruloyl esterase is optimised. • Arabinoxylans with tailored molar mass, substitution and feruloylation are obtained. Wheat bran is an abundant and low valued agricultural feedstock rich in valuable biomolecules as arabinoxylans (AX) and ferulic acid with important functional and biological properties. An integrated bioprocess combining subcritical water extraction (SWE) and enzymatic treatments has been developed for maximised recovery of feruloylated arabinoxylans and oligosaccharides from wheat bran. A minimal enzymatic cocktail was developed combining one xylanase from different glycosyl hydrolase families and a feruloyl esterase. The incorporation of xylanolytic enzymes in the integrated SWE bioprocess increased the AX yields up to 75%, higher than traditional alkaline extraction, and SWE or enzymatic treatment alone. The process isolated AX with tailored molecular structures in terms of substitution, molar mass, and ferulic acid, which can be used for structural biomedical applications, food ingredients and prebiotics. This study demonstrates the use of hydrothermal and enzyme technologies for upcycling agricultural side streams into functional bioproducts, contributing to a circular food system. [ABSTRACT FROM AUTHOR]

[Display omitted] • SAHDBs were established and operated for 215 days. • Room temperature formation filler outperformed melt formation filler in SAHDBs. • The TNRE of Ra biofilter reached 97%, and the proportion of SAD was 78%. • Thiobacillus was the dominant functional genus in the SAHDBs. • Metabolic genes and pathways of C, N, and S compounds were analyzed. Landfill leachate treatment is a major challenge in wastewater treatment. In this study, two sulfur-based autotrophic-heterotrophic denitrification biofilters (Ra biofilter with room-temperature molded filler and Rb biofilter with melt molded filler) were used to treat kitchen-landfill leachate at low temperatures. The effects of reflux ratio, concentrations of NaHCO 3 , and Na 2 S 2 O 3 on the total nitrogen removal efficiency were analyzed, and based on response surface methodology, the optimum parameters were determined. After optimization, the total nitrogen removal efficiency for the Ra and Rb biofilters increased by 83% and 81%, respectively. Moreover, sulfur-based autotrophic denitrification accounted for more than 70% of the nitrogen removal in both biofilters. Based on high-throughput sequencing results, the functional bacteria exhibited high abundance in the Ra biofilter, indicating that the room-temperature molded filler favored the enrichment of functional bacteria. These findings were important for optimizing the operation of sulfur autotrophic-heterotrophic denitrification biofilters at low temperatures. [ABSTRACT FROM AUTHOR]

[Display omitted] • HCl co-hydrothermally treated hydrochar showed high adsorption ability for LVX. • The adsorption of LVX was a spontaneous endothermic process. • Yan model best described the breakthrough behavior of LVX in bioretention column. Hydrochars are promising adsorbents in pollutant removal for water treatment. Herein, hydrochloric acid (HCl) co-hydrothermally treated hydrochars were prepared from rice husk biomass at 180 °C via a one-step hydrothermal method. Adsorption behaviors of levofloxacin (LVX) on hydrochars were evaluated. The specific surface area and pore volume of the hydrochar synthesized in 5 mol/L HCl (5H-HC) were almost 17 and 8 times of untreated hydrochar, respectively. The 5H-HC sample exhibited the highest LVX adsorption capability at room temperature (107 mg/g). Thermodynamic experimental results revealed that adsorption was a spontaneous endothermic process. Yan model provided the best description of the breakthrough behavior of LVX in bioretention column, indicating that the adsorption on the samples involved several rate-limiting factors including diffusion and mass transfer. The results show that facile HCl co-hydrothermal carbonization of waste biomass can produce novel hydrochars with high LVX adsorption ability. [ABSTRACT FROM AUTHOR]

[Display omitted] • Pilot-scale CF-EFBs were constructed for the treatment of low-carbon tailwater. • The TIN purification ability of CF-EFBs for tailwater was 0.96 gm−2d−1. • With the coverage of 30%, CF-EFBs had better TIN removal efficiency. • The carbon fibers can enrich more kinds of nitrogen removal functional microorganisms. A pilot-scale carbon fibers enhanced ecological floating beds (CF-EFBs) was constructed. Compared to EFBs without carbon fibers enhancement, CF-EFBs have the better removal of total inorganic nitrogen (TIN), total phosphorus (TP), and chemical oxygen demand (COD), the removal efficiencies were 3.19, 3.49, and 2.74 times higher than EFBs. Throughout the pilot test (under three different coverage rates), the concentrations of COD, TIN and TP of effluent were 18.11 ± 4.52 mgL−1, 1.95 ± 0.92 mgL−1 and 0.13 ± 0.08 mgL−1. Meanwhile, the average removal of TIN, TP and COD from tailwater was 0.96 gm−2d−1, 0.07 gm−2d−1 and 2.37 gm−2d−1 respectively. When the coverage was 30 %, the CF-EFBs had better nitrogen removal effectiveness (TIN purification ability of 1.49 gm−2d−1). The enrichment of denitrifying bacteria, such as Aridibacter , Nitrospira , Povalibacter , and Phaeodactylibacter increased denitrification efficiency. These results verified the feasibility of CF-EFBs in tailwater treatment at pilot-scale, which was of great significance for the practical application of CF-EFBs. [ABSTRACT FROM AUTHOR]

[Display omitted] • MZVI@CS-added constructed wetland removes nitrate continuously under low C/N. • No passivation of iron particles or microbial cells occurred during operation. • The formation of Fe-CS chelates was the main reason of passivation eliminating. • MZVI@CS enriches iron-autotrophic denitrification microorganisms. • MZVI@CS promotes the high expression of nitrate removal related genes. Reducing nitrate in the secondary effluent from municipal wastewater treatment plants can prevent eutrophication, which can be achieved by constructed wetlands. Zero-valent iron has been used as electron donors for nitrate removal in constructed wetlands to deal with the low carbon-to-nitrogen ratio (C/N) problem, but the effects are often limited by passivation. In this study, micron zero-valent iron chitosan hydrogel balls were prepared as part of the substrate. The total nitrogen removal efficiency maintained at 85 %-96 % in 70 days. The chelating ability of chitosan could reduce the formation of iron oxides on the surface of iron particles and microbial cells, thus eliminating the passivation. Denitrification microorganisms were enriched and the expressions of denitrification genes were increased. The study provides new understandings of further improving the nitrate removal efficiency of constructed wetlands under low C/N and efficient use of iron materials. [ABSTRACT FROM AUTHOR]

[Display omitted] • Anammox and ammonia stripping can provide environmental and economic sustainability. • N 2 O control is key to limiting the environmental impacts of biological processes. • Dirammox, DAMO & electrochemical systems are promising targets for future research. • Stoichiometry & lab-scale data insufficiently describe the environmental impacts. This review critically assesses nitrogen removal technologies applied in the reject water treatment, across different stages of technological development, with a focus on their economic and environmental impacts. The prevalent use of biological processes raises concerns due to potential environmental impacts caused by N 2 O emissions. However, partial nitritation-anaerobic ammonium oxidation demonstrated economic benefits and the potential for positive environmental outcomes when properly operated and controlled. Furthermore, reject water, in many cases, provides sufficient nitrogen concentrations for nitrogen recovery processes, such as ammonia stripping, substituting production of industrial fertilizers and contributing to a circular economy. Nonetheless, their financial competitiveness is subject to various conditions, including the nitrogen concentration or reject water flow. As the environmental benefits of bioprocesses and economic benefits of nitrogen recovery processes may vary, it is crucial to further optimize both and investigate novel promising technologies such as electrochemical systems, denitrifying anaerobic methane oxidation or direct ammonia oxidation. [ABSTRACT FROM AUTHOR]

With advancements in research and the necessity of improving the performance of bioelectrochemical system (BES), coupling anaerobic digestion (AD) with BES is crucial for energy gain from wastewater and bioremediation. Hybridization of BES-AD concept opens new avenues for pollutant degradation, carbon capture and nutrient-resource recovery from wastewater. The strength of merging BES-AD lies in synergy, and this approach was employed to differentiate fads from strategies with the potential for full-scale implementation and making it an energy-positive system. The integration of BES and AD system increases the overall performance and complexity of combined system and the cost of operation. From a technical standpoint, the primary determinants of BES-AD feasibility for field applications are the scalability and economic viability. High potential market for such integrated system attract industrial partners for more industrial trials and investment before commercialization. However, BES-AD with high energy efficacy and negative economics demands performance boost., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The accumulation of phosphorus in activated sludge in wastewater treatment plants (WWTPs) provides potential for phosphorus recovery from sewage. This study delves into the potential for releasing phosphorus from waste activated sludge through two distinct treatment methods-thermal hydrolysis and pH adjustment. The investigation was conducted with activated sludge sourced from four WWTPs, each employing distinct phosphorus removal strategies. The findings underscore the notably superior efficacy of pH adjustment in solubilizing sludge phosphorus compared to the prevailing practice of thermal hydrolysis, widely adopted to enhance sludge digestion. The reversibility of phosphorus release within pH fluctuations spanning 2 to 12 implies that the release of sludge phosphorus can be attributed to the dissolution of phosphate precipitates. Alkaline sludge treatment induced the concurrent liberation of COD, nitrogen, and phosphorus through alkaline hydrolysis of sludge biomass and the dissolution of iron or aluminium phosphates, offering potential gains in resource recovery and energy efficiency., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Xiang Cheng reports financial support was provided by Ministry of Science and Technology of the People’s Republic of China. Xiang Cheng reports financial support was provided by National Natural Science Foundation of China. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The direct integration of anammox process into municipal wastewater treatment has caused widespread concern, but the lack of anammox seeds limited its real application. This study successfully cultivated anammox granules (322.0 μm) from conventional activated sludge treating municipal wastewater. Through ultra-low floc sludge retention times of 8d, nitrifiers on flocs were eliminated and partial nitrification was realized. Furthermore, highly bacteria-enriched granules were initially formed, with Nitrosomonas and Ca. Competibacter 4-fold higher than that of flocs. Specific staining results revealed the microbial interaction with Ca. Brocadia, considering that Ca. Competibacter and Nitrosomonas correspondingly identified in the inner and outer layers of granules. The percentage of Ca. Brocadia present on the granules increased substantially from 0.0 % to 3.0 %, accompanied by a nitrogen removal rate of 0.3 kg·m -3 ·d -1 . Our findings revealed a valuable reference for the anammox bacteria in-situ enrichment under mainstream conditions, which provides theoretical guidance for anammox-based processes practical application., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Large quantities of greenhouse gases (GHGs) are emitted into the atmosphere during wastewater treatment. In this study, GHG and microbial samples were collected from four wastewater treatment plants (WWTPs), and their differences and relationships were assessed. The study showed that, compared with conventionally constructed WWTPs, well-established gas collection systems in underground WWTPs facilitate comprehensive collection and accurate accounting of GHGs. In aboveground WWTPs, capped anoxic ponds promote methane production releasing it at 2-8 times the rate of uncapped emissions, in contrast to nitrous oxide emissions. Moreover, a stable subsurface environment allows for smaller fluctuations in daily GHG emissions and higher microbial diversity and abundance. This study highlights differences in GHG emission fluxes and microbial communities in differently constructed WWTPs, which are useful for control and accurate accounting of GHG emissions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Aerobic digestion remains the preferred choice for small-scale wastewater treatment plants (WWTPs) in some developing countries, largely due to economic viability and operational simplicity. The escalating production of waste activated sludge (WAS) has prompted small-scale WWTPs to improve efficiency. To address this issue, this study employed an in-situ sulfite treatment as a non-intrusive method to augment aerobic digestion. With sulfite-enhanced solubilization and hydrolysis, a 3.6-fold increase in degradation was achieved. Both sludge dewatering properties and pathogens inactivation were improved. Microbial community analysis revealed a preferential enrichment of Actinobacteriota and Firmicutes during sulfite treatment. The desktop scaling-up estimation suggests that implementing this treatment yielded operational cost savings exceeding 40 %. In summary, in-situ sulfite treatment offers a cost-effective strategy for WAS management in small-scale WWTPs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

In this study, the effect of α-Fe 2 O 3 nanoparticles spiking in urban wastewater (UWW) on growth rate, wastewater treatment ability and bioproducts generation of C. vulgaris and Spirulina was investigated and compared with pure cultivation system. The biomass concentration of C. vulgaris and Spirulina improved by 20 % and 39 % at 10 and 15 mg/L α-Fe 2 O 3 , respectively while the both microalgae growth pattern fitted better with Gompertz simulation after treatment with α-Fe 2 O 3 . The nutrients mass balance revealed that 1 g of treated C. vulgaris and Spirulina could uptake more COD, TN and TP in comparison to the untreated cells. The lipid generation increased remarkably (C. vulgaris: 45 % and Spirulina: 72 %) after α-Fe 2 O 3 treatment. While, the addition of α-Fe 2 O 3 showed no significant impact on the protein and carbohydrate productivity. Overall, this study evangelize the role of nanoparticles on promoting microalgae applications as a sustainable approach for UWW treatment and promising feedstock for biofuel production., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

The implementation of the anaerobic ammonium oxidation (anammox) process in treating low-strength wastewater is limited by the difficulty in enriching anammox bacteria (AnAOB). Here, the first enrichment of AnAOB at a high nitrogen (N) loading rate (NLR) as a strategy was proposed to achieve the rapid start-up of the anammox biofilm process treating low-strength wastewater. The long-term stability of the anammox biofilm process after start-up operating at a low NLR of 0.2-0.4 kg N/(m 3 ⋅d) was evaluated. Results showed that the N removal efficiency was up to 75 % under a low NLR of 0.2 kg N/(m 3 ⋅d) condition. Low-strength organic matter promoted the metabolic coupling between partial denitrifying bacteria (PDB) and AnAOB. The genus Candidatus Brocadia as AnAOB (18 %-27 %) can coexist with Limnobacter (PDB, 9 %-12 %) for efficient N removal. This study offers a rapid start-up strategy of anammox biofilm process in treating low-strength wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Applications of post-denitrification processes are subjected to low reaction rates caused by a lack of carbon resources. To offer a solution for reaction rate promotion, this research found a pilot-scale anaerobic/aerobic/anoxic bioreactor treating 55-120 m 3 /d low-strength municipal wastewater for 273 days. A short hydraulic retention time (HRT, 5-6 h) and a high nitrogen removal rate (63.2 ± 9.3 g-N/m 3 ·d) were achieved using HRT optimization. The effluent total nitrogen concentration was maintained at 5.8 ± 1.4 mg/L while operating at a high nitrogen loading rate of 86.2 ± 12.8 g-N/m 3 ·d. The short aeration (1.25-1.5 h) minimized the Glycogen loss. The endogenous denitrification rate increased to above 1.0 mg/(g-VSS·h). The functional genus Ca. Competibacter enriched to 2.3 %, guaranteeing the efficient post-denitrification process. Dechloromonas rose to 1.1 %, aiding in the synchronous phosphorus removal. These findings offered fresh insights into AOA processes to achieve energy/cost-saving wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

The present study evaluated the growth, self-flocculation, lipid content, and pollutants removal by Limnothrix sp. BASMWW-9 isolated from municipal wastewater treatment system and cultivated in municipal wastewater. The biomass yield and lipid content after 6 days of cultivation were 1.07 g dw/L and 27.34 %dw, respectively. In addition, its self-flocculating ability reached up to 90 % after harvesting time of 180 min. Moreover, COD,NH 3 -N, TN, and TP removalefficiencies were 71.65 %, 81.89 %, 74.64 %, and 80.16 %, respectively. The self-flocculation performance of Limnothrix sp. was greatly associated to its morphology and production of extracellular polymeric substances (EPS), with significant positive impact of the high calcium and magnesium content in municipal wastewater. Interestingly, blue light irradiation during harvest enhanced the aggregation and floc formation as a floating biomat, which was attributed to enhanced polysaccharides production. This study provides innovative harvest method for Limnothrix sp. BASMWW-9 cultivated in wastewater using blue light for enhanced lipid recovery., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

In this study, an anaerobic/intermittently-aerated moving bed biofilm reactor (AnIA-MBBR) was proposed to realize simultaneous nitrification and endogenous denitrifying phosphorus removal (SNEDPR) in treating low carbon-to-nitrogen (C/N) ratio wastewater. The effect of different intermittent aeration modes (short and long aeration) on nutrients' removal was investigated. With the C/N ratio around 3, the removal efficiencies of total nitrogen and phosphorus were 90% and 74%, 88% and 59%, respectively, for short aeration and long aeration. The different aeration time also altered the nutrients' degradation pathway, biofilm characteristics, microbial community, and functional metabolic pathways. The results confirmed the occurrence of aerobic denitrifiers, anoxic denitrifiers, phosphorus accumulating organisms, glycogen accumulating organisms in AnIA-MBBR systems and their synergistic performance induced the SNEDPR. These results indicated that the application of AnIA in MBBR systems was an effective strategy to achieve SNEDPR, providing better simultaneous removal performance of nitrogen and phosphorus from low C/N ratio wastewater., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Precisely predicting the concentration of nitrogen-based pollutants from the wastewater treatment plants (WWTPs) remains a challenging yet crucial task for optimizing operational adjustments in WWTPs. In this study, an integrated approach using factor analysis (FA) and machine learning (ML) models was employed to accurately predict effluent total nitrogen (Ntot eff ) and nitrate nitrogen (NO 3 -N eff ) concentrations of the WWTP. The input values for the ML models were honed through FA to optimize factors, thereby significantly enhancing the ML prediction accuracy. The prediction model achieved a highest coefficient of determination (R 2 ) of 97.43 % (Ntot eff ) and 99.38 % (NO 3 -N eff ), demonstrating satisfactory generalization ability for predictions up to three days ahead (R 2  >80 %). Moreover, the interpretability analysis identified that the denitrification factor, the pollutant load factor, and the meteorological factor were significant. The model framework proposed in this study provides a valuable reference for optimizing the operation and management of wastewater treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The circular bioeconomy (CBE) presents a sustainable solution for the pig farming system, delivering economic and environmental benefits. This shift from a linear to a CBE model is anticipated to result in substantial economic, environmental, and social transformations. In this study, the CBE outcomes are evaluated with Scenarios (1 to 3): (1) pig farming and anaerobic digestion (AD) only, (2) pig farming, AD, and microalgae system (MS) with partial microalgae-based biomass (MB) recycle, and (3) pig farming, AD, and MS without MB recycle. Through economic and life cycle analyses, the internal rate of return for Scenarios (1 to 3) are 13.3%, 15.0%, and 12.3%, respectively, but the corresponding endpoint indicators are 483pt, 363pt, and 398pt. To address the best CBE, Scenario 2 by using MB product as a pig feed supplement could achieve higher revenue as well as lower environmental impact., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The use of microalgae-bacteria consortia (MBC) for wastewater treatment has garnered attention as their interactions impart greater environmental adaptability and stability compared with that obtained by only microalgae or bacteria use, thereby improving the efficiency of pollutant removal and bio-product productivity. Additionally, the value-added bio-products produced via biorefineries can improve economic competitiveness and environmental sustainability. Therefore, this review focuses on the interaction between microalgae and bacteria that leads to nutrient exchange, gene transfer and signal transduction to comprehensively understand the interaction mechanisms underlying their strong adaptability. In addition, it includes recent research in which MBC has been efficiently used to treat various wastewater. Moreover, the review summarizes the use of MBC-produced biomass in a biorefining context to produce biofuel, biomaterial, high-value bio-products and bio-fertilizer. Overall, more effort is needed to identify the symbiotic mechanism in MBC to provide a foundation for circular bio-economy and environmentally friendly development programmes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This work investigated the impact of a waste-derived carbon source, crude glycerol (CG), on Anammox. Batch bioassays were conducted to identify inhibitory component(s) in CG, and the relationship between Anammox activity and the concentration of CG, pure glycerol, and methanol were assessed. The results showed that the half-maximal inhibitory concentration of CG and methanol are 434.5 ± 51.8 and 143.0 ± 19.6 mg chemical oxygen demand (COD) L -1 , respectively, while pure glycerol at 0-2283 mg COD L -1 had no significant adverse effect on Anammox. The results suggested methanol is the major inhibitor in CG via a non-competitive inhibition mechanism. COD/total inorganic nitrogen ratio of > 1.3 was observed to cause a significant Anammox inhibition (>20 %), especially at low substrate level. These results are valuable for evaluating the feasibility of using CG for nitrogen removal in water resource recovery facilities, promoting sustainable development., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Biochar is a stable carbonaceous material derived from various biomass and can be utilized as adsorbents, catalysts and precursors in various environmental applications. This review discusses various feedstock materials and methods of biochar production via traditional as well as modern approaches. Additionally, the biochar characteristics, HTC process, and its modification by employing steam and gas purging, acidic, basic / alkaline and organo-solvent, electro- and magnetic fields have been discussed. The recent biochar applications for real water, wastewater and industrial wastewater for the abstraction of environmental contaminants also reviewed. Moreover, applications in machine learning and microbial sensors were discussed. In the meantime, analyses on commercial and environmental profit, current ecological concerns and the future directions of biochar application have been well presented., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The proliferation of novel wastewater treatment processes has marked recent years, becoming particularly pertinent in light of the strive for carbon neutrality. One area of growing attention within this context is nitrous oxide (N 2 O) production and emission. This review provides a comprehensive overview of recent research progress on N 2 O emissions associated with novel wastewater treatment processes, including Anammox, Partial Nitrification, Partial Denitrification, Comammox, Denitrifying Phosphorus Removal, Sulfur-driven Autotrophic Denitrification and n-DAMO. The advantages and challenges of these processes are thoroughly examined, and various mitigation strategies are proposed. An interesting angle that delve into is the potential of endogenous denitrification to act as an N 2 O sink. Furthermore, the review discusses the potential applications and rationale for novel Anammox-based processes to reduce N 2 O emissions. The aim is to inform future technology research in this area. Overall, this review aims to shed light on these emerging technologies while encouraging further research and development., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

[Display omitted] • Efficient and sustainable PiNe–SO 3 H adsorbent for methylene blue removal from wastewater. • Kinetics, thermodynamics and isotherms aimed at the identification of methylene blue adsorption mechanism on PiNe–SO 3 H. • Identification of the key role of surface functional groups on PiNe–SO 3 H in methylene blue up take. • Definition of rapid and waste-minimized protocol for the PiNe–SO 3 -methylene blue regeneration. • Achievement of optimal balance between maximum adsorption efficiency and minimum waste generation. In pursuit of sustainable water management, the preparation of adsorbent materials via waste upcycling for water purification practices plays a decisive role. The sulphonated biochar, PiNe–SO 3 H, employed to target the methylene blue dye adsorption, was successfully synthesized via a mild, step-economical chemical carbonization-functionalization reaction. The presence of SO 3 H groups on the PiNe–SO 3 H surface played a critical role in significantly enhancing the adsorption capacity. The observed MB dye uptake was predominantly attributed to chemisorption processes as evidenced by the results from kinetics, thermodynamics, and isotherms. To further confirm the role of -SO 3 H in the adsorption mechanism, a comparison was made with other PiNe materials lacking sulphonic groups, highlighting the superior adsorption capacity of PiNe–SO 3 H. Additionally, a fast and efficient regeneration process was proposed to develop a truly waste minimized protocol, enabling the recovery of up to 94 % of the ethanolic mixture used during this step. [ABSTRACT FROM AUTHOR]

[Display omitted] • An MBR system was optimized to decrease operational costs and GHG emissions. • Effluent quality in all trials complied with the EU water reuse legislation. • The airflow rate had a substantial impact on the operational costs. • Increased airflow rate decreased N 2 O concentrations in the liquid phase. • The lowest airflow rate and highest backwash frequency was selected as the optimum. A trade-off between greenhouse emissions (direct and indirect) and operational costs in the water treatment sector is of great importance, although only few literature studies exist. The paper presents a comprehensive experimental study on a Membrane Bioreactor (MBR) pilot plant at the Water Resource Recovery Facility of Palermo University (Italy). The MBR pilot plant was aimed at reducing carbon footprint while producing water suitable for water reuse in agriculture. Multiple scenarios were assessed to unveil the best operational variables including the assessment of the reclaimed water quality index for water reuse. Results showed the lowest operational costs for the MBR of 5.05 € cent/m3 with Class B according to 741/2020 European legislation. Results revealed optimised values, in terms of airflow rate and backwash frequency, of 0.8 m3/m2/h and 12 times/h, respectively. The highest N 2 O emission was measured in correspondence of scenario S5 (airflow rate of 1.6 m3/m2/h) with 0.40 mg N 2 O-N/m2/h in agreement with previous literature studies. The obtained results could effectively address the operators to find a trade-off between operational costs and water quality. [ABSTRACT FROM AUTHOR]

[Display omitted] • Modification protocols of sodium alginate are reviewed. • Physical modification including blending is easy but has strength concerns. • Chemical modification can yield stable matrix with functions. • Novel modification protocols and applications are outlined. Sodium alginate is a natural macromolecule widely used because of its abundance, low cost of acquisition, and rich hydroxyl and carboxyl groups in the matrix. The physical modification of sodium alginate can be made by blending it with polymer materials. The so-yielded alginate complex is commonly unstable in an aqueous environment due to alginate backbones' high hydrophilicity. The chemical modification can remove its hydrophilic groups and introduce special functional groups or polymers onto the alginate backbones to provide excess reaction sites for specific reactions and effective complexation sites for accommodating antibiotics, dyes, heavy metal ions, and radioactive elements. Sodium alginate has been used in water treatment engineering under revised modification protocols. This article also reviews the latest modification protocols for sodium alginate and outlines the novel application of the modified materials. The limitations of modified sodium alginate materials are described, and research prospects are put forward. [ABSTRACT FROM AUTHOR]

[Display omitted] • Global optimisation of industrial effluent treatment by Diplosphaera mucosa. • Hybridisation of RSM and ANN models with GA for multi-input optimisation. • Application of both MATLAB and Python for ANN model construction. • Two-way interaction of pH and N/P ratio has significant effect on algae growth. • ANN models generated by Python showed better accuracy. The wastewater treatment efficiency of Diplosphaera mucosa VSPA was enhanced by optimising five input parameters and increasing the biomass yield. pH, temperature, light intensity, wastewater percentage (pollutant concentration), and N/P ratio were optimised, and their effects were studied. Two competitive techniques, response surface methodology (RSM) and artificial neural network (ANN), were applied for constructing predictive models using experimental data generated according to central composite design. Both MATLAB and Python were used for constructing ANN models. ANN models predicted the experimental data with high accuracy and less error than RSM models. Generated models were hybridised with a genetic algorithm (GA) to determine the optimised values of input parameters leading to high biomass productivity. ANN-GA hybridisation approach performed in Python presented optimisation results with less error (0.45%), which were 7.8 pH, 28.8 °C temperature, 105.20 μmol m−2 s−1 light intensity, 93.10 wastewater % (COD) and 23.5 N/P ratio. [ABSTRACT FROM AUTHOR]

[Display omitted] • MoS 2 nanosheets were coated on biochar under the mediation of PDA. • The biochar-based composite exhibits highly efficient removal of RhB and Pb(II). • It was proven to be reusable and practicable for water treatment. • The mechanism of RhB and Pb(II) adsorption is elucidated through multiple approaches. Mediated by polydopamine, MoS 2 nanosheets were immobilized on the porous biochar derived from fungus residue, forming a novel biochar-based nanocomposite (MoS 2 –PDA@FRC) for the removal of Rhodamine B(RhB) and Pb(II) from water. Utilizing MoS 2 nanosheets with abundant active adsorption sites, MoS 2 –PDA@FRC showed higher adsorption capacities than raw biochar, with 2.76 and 1.78 times higher capacities for RhB and Pb(II) respectively. MoS 2 –PDA@FRC also exhibited fast adsorption kinetics for RhB (120 min) and Pb (180 min) removal, as well as satisfactory adsorption selectivity in the presence of coexisting substances. The underlying removal mechanism was explored via Fourier transform infrared and X-ray photoelectron spectroscopies. Furthermore, during cyclic adsorption–regeneration and the fixed-bed adsorption experiments, the nanocomposite removed RhB and Pb(II) with high effectiveness and stability. Collectively, the results demonstrated the bright prospects of MoS 2 –PDA@FRC as a highly efficient decontamination agent of RhB and Pb(II) from water. [ABSTRACT FROM AUTHOR]

[Display omitted] • Three antibiotics had different removal trends in a concentration-dependent manner. • The three antibiotics inhibited the growth of microalgae differently. • Enzymes activity levels of antioxidants changed in response to antibiotics exposure. • The cytochrome p450 can play an important role in the removal of antibiotics. • The operation costs of microalgae were estimated to be 4.93€/m3. Microalgae have attracted increasing attention as an environmentally friendly treatment for antibiotics. However, the effect of antibiotic concentration on the removal ability of microalgae with the underlying mechanisms remains unclear. Thus, this work investigates the removal of tetracycline (TET), sulfathiazole (STZ), and ciprofloxacin (CIP) at different concentrations using Chlorella sorokiniana. The results indicate that microalgae have a concentration-dependent effect on antibiotic removal; however, the removal trends for the three antibiotics differed significantly. Specifically, TET showed nearly 100% removal efficiency at any concentration. The high concentration of STZ inhibited microalgal photosynthesis and induced the production of ROS, leading to antioxidant damage and inhibiting removal efficiency. Conversely, CIP enhanced the ability of microalgae to remove CIP by inducing a dual peroxidase and cytochrome p450 enzyme response. Furthermore, the economic analysis demonstrated that microalgae treatment antibiotics were calculated to be 4.93€/m3, which becomes cheaper than the other microalgae water treatment process. [ABSTRACT FROM AUTHOR]

Effective separation of volatile fatty acids (VFAs), ammonia (NH 4 + -N) and reactive phosphorous (RP) generated from anaerobic fermentation liquid is critically important for efficient resource recovery. Flow-electrode capacitive deionization (FCDI) is proven to be capable of efficient removal of ions, environmentally friendly and cost-effective in operation. The performances of FCDI system in the separation of NH 4 + -N, RP, and acetate and mechanism of pHs and activated carbon on their performances were investigated. Results showed that a pH of 5.0 promoted the removal of NH 4 + -N (53.1 %) and RP (39.5 %), and 72.0 % of acetate was retained in the solution, which revealed that removal of NH 4 + -N and RP, and retention of acetate were evidently affected by speciation of ions. Furthermore, the recovery of NH 4 + -N and RP was undermined by the adsorption of ions on activated carbon. This study provides a novel insight of ion selective mechanism during the operation of the FCDI system., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The effective control of total nitrogen (ETN) and total phosphorus (ETP) in effluent is challenging for wastewater treatment plants (WWTPs). In this work, automated machine learning (AutoML) (mean square error = 0.4200 ∼ 3.8245, R 2  = 0.5699 ∼ 0.6219) and back propagation artificial neural network (BPANN) model (mean square error = 0.0012 ∼ 6.9067, R 2  = 0.4326 ∼ 0.8908) were used to predict and analyze biological nutrients removal in full-scale WWTPs. Interestingly, BPANN model presented high prediction performance and general applicability for WWTPs with different biological treatment units. However, the AutoML candidate models were more interpretable, and the results showed that electricity carbon emission dominated the prediction. Meanwhile, increasing data volume and types of WWTP hardly affected the interpretable results, demonstrating its wide applicability. This study demonstrated the validity and the specific advantages of predicting ETN and ETP using H 2 O AutoML and BPANN model, which provided guidance on the prediction and improvement of biological nutrients removal in WWTPs., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Magnetic biochar (MBC) is a novel bio-carbon material with both desired properties as adsorbent and magnetic characteristics. This review provides an up-to-date summary and discussion on the latest development of MBC, which covers the progress on its synthesis, application, and techno-economic analysis. The review indicates that the direct hydrothermal synthesis has been catching more research attention to produce MBC due to its mild reaction conditions. Instead of the Fe-loaded MBC, there is a trend of using Mn for the magnetization. For the MBC application, how to improve its adsorption performance for water decontamination, ideally to match that of the biochar (BC) or activated carbon, is important. In addition, more studies on the environmental impacts of MBC and life-cycle assessment decoding the process optimization options are necessary. This review will provide valuable references for the development of MBC and MBC-based materials for wastewater treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

Advanced sustainable bioremediation is gaining importance with rising global pollution. This review examines microalgae's potential for sustainable bioremediation and process enhancement using multi-omics approaches. Recently, microalgae-bacterial consortia have emerged for synergistic nutrient removal, allowing complex metabolite exchanges. Advanced bioremediation requires effective consortium design or pure culture based on the treatment stage and specific roles. The strain potential must be screened using modern omics approaches aligning wastewater composition. The review highlights crucial research gaps in microalgal bioremediation. It discusses multi-omics advantages for understanding microalgal fitness concerning wastewater composition and facilitating the design of microalgal consortia based on bioremediation skills. Metagenomics enables strain identification, thereby monitoring microbial dynamics during the treatment process. Transcriptomics and metabolomics encourage the algal cell response toward nutrients and pollutants in wastewater. Multi-omics role is also summarized for product enhancement to make algal treatment sustainable and fit for sustainable development goals and growing circular bioeconomy scenario., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Waste molasses, the abundant byproducts of the sugar industry, is a cost-efficient carbon source for advanced denitrification. However, the efficiency of waste molasses-driven denitrification is limited by its complex carbon content, hindering its practical application. Weak magnetic field (WMF) is reported to enhance biological nitrogen removal, but its effects on molasses-driven denitrification remains unknown. This study investigated whether the WMF can enhance waste molasses-driven nitrogen removal and explore the underlying mechanisms. It was found that WMF significantly facilitated waste molasses-driven denitrification, with total nitrogen removal efficiency increased by 1.25 times (from 77% to 96%). WMF stimulated the nitrate reductase's activity by 7-18%, and the enhancement was improved as WMF intensified. Quantitative qPCR analysis indicated that the abundances of denitrifying enzymes increased under WMF, which was consistent with the proliferation of denitrifying bacteria Denitratisoma and Devosia. This study has demonstrated that WMF is promising for enhancing complex carbon-driven denitrification processes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Ltd.)

In this study, a novel fungal-algal coupling system was established for slaughterhouse wastewater treatment with Chlorella sp. DT025 and a new fungus, Penicillium sp. AHP141. With the optimization of cultivation conditions for the fungal-algal coupling system, the harvest efficiency of Chlorella sp. DT025 reached 99.79%. The mechanism of microalgae harvest of the fungal-algal system was revealed to be related to the morphological characteristics, surface charge, and the secretion of humic acid-like compounds and tryptophan on the surface of the fungi cells. For the original slaughterhouse wastewater treatment, the fungal-algal coupling system had a better removal efficiency of COD, TN, and TP than both monoculture systems. In the high-concentration artificial slaughterhouse wastewater, COD removal of the fungal-algal system reached more than 5350 mg/L. The lipid production of the fungal-algal coupling system in the high-concentration artificial slaughterhouse wastewater treatment was improved by 343.33% to 1.33 g/L compared to the microalgae monoculture treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

By regulating influent nitrogen loading rate (NLR) and solids retention time (SRT), the effect of specific biomass nitrogen load (BNL) on the start-up of mainstream partial nitrification (PN) was investigated in five parallel sequencing batch reactors inoculated with ordinary nitrification sludge. The results showed that increasing BNL by both methods could achieve nitrite accumulation. Moreover, a high initial activity of ammonium oxidizing bacteria (AOB) accelerated nitrite accumulation. Increasing influent NLR (ammonium: 55-70 mg N/L) achieved only 30%-40% of nitrite accumulation ratio (NAR) and gradually decreased with reactor operation. By increasing BNL via controlling SRT (30 days), desirable PN with an average NAR of 81.7 ± 4.4% (effluent nitrite: ∼10 mg N/L) was obtained. Nitrite oxidizing bacteria (NOB) were effectively inhibited, and the AOB to NOB activity ratio increased from 1.5 to 7.8, promoting efficient nitrite accumulation. Overall, increasing BNL by regulating SRT was a potential method for start-up of mainstream PN., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

The treatment and reuse of hygiene wastewater is crucial to "close the loop" in the controlled ecological life support system (CELSS), and to guarantee longer space missions or planetary habitation. In this work, anaerobic membrane bioreactor (AnMBR) was applied for hygiene wastewater treatment, focused on surfactant degradation and microbial community succession. The removal efficiency of COD and surfactants was 90%∼97% and 80% with a urine source-separation strategy. The microbial community gradually shifted from methanogens to sulfur-metabolizing and surfactant-degradation bacteria, such as Aeromonas. Sulfate was a surfactant degradation product, which triggered sulfate reduction and methane inhibition. The activated carbohydrate and sulfur metabolism were the key mechanism of the microbial process for the excellent performance of AnMBR. This study analyzed the degradation mechanism from the perspective of microbial mechanism, offers a solution for CELSS hygiene wastewater treatment, and supports the future improvement and refinement of AnMBR technology., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Modern paradigm has upgraded wastewater treatment plants (WWTPs) to water resources recovery facilities (WRRFs), where aerobic granular sludge (AGS) is a sewage treatment technology with promising phosphorus recovery (PR) potential. Herein, the AGS-based simultaneous nitrification, denitrification, and phosphorus removal coupling side-stream PR process (AGS-SNDPRr) was developed with municipal wastewater. Results revealed that AGS always maintained good structural stability, and pollutant removal was unaffected and effective after 40 days of anaerobic phosphorus-rich liquid extraction (fixed rate of 30%). The AGS-SNDPRr achieved a stable phosphorus recovery efficiency of 63.40%, and the side-stream PR further exaggerated in situ sludge reduction by 7.7-10%. Apart from responses of extracellular polymeric substances (EPS), the Matthew effect of typical denitrifying glycogen accumulating organisms (DGAOs) Candidatus_Competibacter up to 67.40% mainly contributed to enhanced performance of this new process. This study demonstrated a new approach for simultaneous advanced wastewater treatment, phosphorus recovery, and excess sludge minimization., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Graphical abstract Highlights • Rape straw- and corn cob-derived porous carbons were prepared and characterized. • KHCO 3 activation of hydrochar was an efficient method to develop pore structure. • The porous carbons exhibited superior sorption capabilities to PAHs. • Possible sorption mechanisms were explained by kinetic and isotherm studies. Abstract In this study, rape straw- and corn cob-derived porous carbons (PCs) were fabricated by hydrothermal treatment (250 °C, 4 h) and subsequent activation (850 °C, 1 h) using a non-corrosive agent, potassium bicarbonate. The PCs exhibited a very large specific surface area (1069–1281 cm2 g−1), high pore volume (0.55–0.72 cm3 g−1), wide pore size distribution (from micropores to macropores), high hydrophobicity, and partly graphitized structure. These properties contributed to highly efficient performance for the sorption of polycyclic aromatic hydrocarbons (PAHs), with maximum sorption capacities of 592.97, 480.27, and 692.27 mg g−1 towards naphthalene, acenaphthene, and phenanthrene, respectively. A three-step sorption process with pore filling, hydrophobic effects, and π-π stacking interactions on the heterogeneous surface is a possible mechanism for the sorption of PAHs onto PCs. This study presents an environmentally friendly strategy for the reuse of crop residues in the field of organic micropollutant-contaminated water treatment. [ABSTRACT FROM AUTHOR]