1. Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance
- Author
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Hui Li, Ji-Dong Gu, Xiao Ming Du, Qiang Lu, Ping Wang, Shu Guang Lu, Xiaoli Wang, Yong Di Liu, Kuang Fei Lin, and Ting Ting Shen
- Subjects
Tetrachloroethylene ,Environmental Engineering ,Firmicutes ,Population ,Molecular Sequence Data ,Bioengineering ,Actinobacteria ,Microbiology ,chemistry.chemical_compound ,RNA, Ribosomal, 16S ,Food science ,Cloning, Molecular ,education ,Waste Management and Disposal ,Phylogeny ,Chlorophenol ,education.field_of_study ,biology ,Sewage ,Renewable Energy, Sustainability and the Environment ,General Medicine ,Biodegradation ,biology.organism_classification ,Phosphoric Monoester Hydrolases ,Activated sludge ,Biodegradation, Environmental ,chemistry ,Microbial population biology ,Synergistetes ,Oxidoreductases ,Chlorophenols - Abstract
This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5–150 mg/L) was mixed with 2-CP (in the range of 25–150 mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150 mg/L. PCR–DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes , Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.
- Published
- 2012