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The pH control to promote short-chain fatty acids (SCFAs) production during anaerobic alkaline fermentation basically focused on constant pH control. In this study, a simple and consumption-reducing intermittent pH control strategy at moderate temperature (23 ± 2 °C) was investigated with adjusting pH to 10 when naturally reduced to 8. The intermittent pH control strategy could alleviate the inhibition of acid-producing bacteria by strong alkaline and high FA concentration. Meanwhile, microbial diversity promoted by 6% and 69% while the relative abundance of acid-producing bacteria increased by 36% and 61% compared to blank and constant pH fermenters. The relative genes abundance related to amino acid metabolism and fatty acid production were mostly promoted and led to enhanced SCFAs production. In the long-term fermenter, the intermittent pH control strategy could result in a 68% reduction in alkali consumption and a 37% increase in SCFAs production compared to that of the constant pH at 10.

In this study, a novel strategy for nitritation establishment through external fermented sludge addition is proposed. The nitrifying activities under various fermentation times were assessed; batch tests results revealed that the decay rate of nitrite oxidizing bacteria (0.53 ± 0.08 d−1) was much higher than that of ammonia oxidizing bacteria (0.15 ± 0.04 d−1), during alkaline fermentation with high endogenous free ammonia concentrations (0.57–138.79 mg·L−1). The long-term effects of fermented sludge addition were constantly monitored in an anaerobic/oxic/anoxic sequencing batch reactor. During 60 days of operation, the nitrite accumulation rate reached above 90% and could be maintained stably. Total inorganic nitrogen removal achieved 94.7%, with an average external sludge reduction rate of 61.3%, after incorporation of the sludge addition. Illumina MiSeq sequencing demonstrated that nitrite oxidizing bacteria were successfully eliminated and the relative abundance of ammonia oxidizing bacteria remained at 0.39%, resulting in nitrite accumulation.

A continuous-flow anaerobic/aerobic/anoxic reactor with complete suspended activated sludge using sludge alkaline fermentation products as carbon source was utilized to strengthen nitrogen removal performance for low C/N ratio (4) wastewater. Long-time performance indicated that the nitrite accumulation rate reached 60.40%, which strengthened the contribution of anammox. The average total inorganic nitrogen removal efficiency improved 19.40%. The abundance of ammonia oxidizing bacteria has not changed, but the abundance of nitrite oxidizing bacteria reduced from 5.79% to 0.69%. Quantitative PCR results demonstrated that the abundance of anammox bacteria has raised by 80.5 times. These results indicated that side-stream sludge alkaline fermentation promoted the mainstream partial nitrification, consequently accelerating the in-situ enrichment of anammox bacteria. No external carbon source dosing and short oxic hydraulic retention time (5.3 h) save energy and reduce consumption significantly in this system.

An anaerobic-aerobic-anoxic sequencing batch reactor (AOA-SBR) using sludge fermentation products as carbon source was developed to enhance nitrogen and phosphorus removal in municipal wastewater with low C/N ratio (

This study presents a novel strategy to improve the removal efficiency of nitrogen and phosphorus from municipal wastewater by feeding sequencing batch reactor (SBR) with sludge alkaline fermentation products as carbon sources. The performances of two SBRs treating municipal wastewater (one was fed with sludge fermentation products; F-SBR, and the other without sludge fermentation products; B-SBR) were compared. The removal efficiencies of total nitrogen (TN) and phosphorus (PO43−-P) were found to be 82.9% and 96.0% in F-SBR, while the corresponding values in B-SBR were 55.9% (TN) and −6.1% (PO43−-P). Illumina MiSeq sequencing indicated that ammonium-oxidizing bacteria (Nitrosomonadaceae and Nitrosomonas) and denitrifying polyphosphate accumulating organisms (Dechloromonas) were enriched in F-SBR, which resulted in NO2−-N accumulation and denitrifying phosphorus removal via nitrite (DPRN). Moreover, feeding of sludge fermentation products reduced 862.1 mg VSS/d of sludge in the F-SBR system (volume: 10 L).