1. Effects of rhamnolipid on the cellulase and xylanase in hydrolysis of wheat straw.
- Author
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Wang HY, Fan BQ, Li CH, Liu S, and Li M
- Subjects
- Carbohydrates analysis, Cellulase drug effects, Fermentation drug effects, Hydrolysis, Lignin analysis, Penicillium enzymology, Sodium Hydroxide pharmacology, Sulfuric Acids pharmacology, Triticum drug effects, Cellulase metabolism, Glycolipids pharmacology, Triticum metabolism, Waste Products analysis
- Abstract
The effects of biosurfactant rhamnolipid (RL) and chemical surfactant Triton X-100 on the production of cellulases and xylanase from Penicillium expansum (P. expansum) in untreated, acid- and alkali-pretreated wheat straw submerged fermentations were studied, and the influences on the activity and stability of Cellulase R-10 were also investigated. The results showed that RL and Triton X-100 enhanced the activities of cellulases and xylanase to different extents and the stimulatory effects of RL were superior to those of Triton X-100. During the peak enzyme production phase, RL (60 RE mg/l) increased cellulases activities by 25.5-102.9%, in which the raise of the same enzyme in acid-pretreated straw broths was the most. It was found that the reducing sugars by hydrolyzing wheat straw with Cellulase R-100 were not visibly increased after adding RL. However, it distinctly protected Cellulase R-10 from degradation or inactivation, keeping the reducing sugars yield at about 17%., (Copyright © 2011. Published by Elsevier Ltd.)
- Published
- 2011
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