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213 results on '"CHEN, CHUN"'

46. Optimization of lutein production with a two-stage mixotrophic cultivation system with Chlorella sorokiniana MB-1

Author

Chun Yen Chen and Chen Chun Liu

Subjects
0106 biological sciences, Lutein, Environmental Engineering, Biomass, Bioengineering, Chlorella, 010501 environmental sciences, 01 natural sciences, chemistry.chemical_compound, 010608 biotechnology, Microalgae, Cultivation System, Food science, Bioprocess, Waste Management and Disposal, 0105 earth and related environmental sciences, Chlorella sorokiniana, Renewable Energy, Sustainability and the Environment, Chemistry, General Medicine, Lipids, Productivity (ecology), Batch Cell Culture Techniques, Aeration, Mixotroph
Abstract

The major purpose of this study was to investigate the effects of operational factors and bioprocess strategies on the mixotrophic cultivation of a microalgae Chlorella sorokiniana MB-1 for lutein production. Aeration with CO2 showed the highest biomass productivity and lutein productivity of 0.89 g/L/d and 3.49 mg/L/d, respectively. Semi-batch operation performed with 80% medium replacement ratio resulted in the highest biomass productivity and lutein productivity of 1.55 g/L/d and 5.51 mg/L/d, respectively. A two-stage strategy was developed to enhance the biomass production of the MB-1 strain in stage 1 with semi-batch mixotrophic culture and to optimize lutein accumulation in stage 2 under photoautotrophic conditions. The maximum biomass productivity and lutein productivity was 1.98 g/L/d and 7.62 mg/L/d, respectively, with a medium replacement ratio of 80% in stage 1. Compared with batch cultivation, the lutein productivity was enhanced by 32.7% for semi-batch operation alone and 85.9% for the semi-batch-integrated two-stage process.

Published
2018

49. Effects of nitrogen source availability and bioreactor operating strategies on lutein production with Scenedesmus obliquus FSP-3

Author

Ming Chang Chan, Chieh-Chen Huang, Jo Shu Chang, Duu-Jong Lee, Shih-Hsin Ho, Chen Chun Liu, Youping Xie, and Chun Yen Chen

Subjects
Lutein, Time Factors, Environmental Engineering, Light, Nitrogen, chemistry.chemical_element, Biomass, Bioengineering, Calcium nitrate, chemistry.chemical_compound, Bioreactors, Botany, Bioreactor, Food science, Nitrogen source, Waste Management and Disposal, Renewable Energy, Sustainability and the Environment, Chemistry, food and beverages, General Medicine, eye diseases, Productivity (ecology), Batch Cell Culture Techniques, Scenedesmus obliquus, Scenedesmus
Abstract

In this study, the effects of the type and concentration of nitrogen sources on the cell growth and lutein content of an isolated microalga Scenedesmus obliquus FSP-3 were investigated. With batch culture, the highest lutein content (4.61 mg/g) and lutein productivity (4.35 mg/L/day) were obtained when using 8.0 mM calcium nitrate as the nitrogen source. With this best nitrogen source condition, the microalgae cultivation was performed using two bioreactor strategies (namely, semi-continuous and two-stage operations) to further enhance the lutein content and productivity. Using semi-continuous operation with a 10% medium replacement ratio could obtain the highest biomass productivity (1304.8 mg/L/day) and lutein productivity (6.01 mg/L/day). This performance is better than most related studies.

Published
2015

50. Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies

Author

Jo Shu Chang, Duu-Jong Lee, Chen Chun Liu, Shih-Hsin Ho, Chun Yen Chen, Ming Chang Chan, and Wen Lung Lee

Subjects
endocrine system, Lutein, Time Factors, Environmental Engineering, Light, Nitrogen, Bioengineering, Photosynthesis, Photobioreactors, chemistry.chemical_compound, Pigment, Light source, Botany, Microalgae, Biomass, Food science, Waste Management and Disposal, Renewable Energy, Sustainability and the Environment, Chemistry, food and beverages, General Medicine, Carbon Dioxide, Production efficiency, eye diseases, Light intensity, Productivity (ecology), Batch Cell Culture Techniques, Scenedesmus obliquus, visual_art, visual_art.visual_art_medium, sense organs, Scenedesmus
Abstract

Lutein, one of the main photosynthetic pigments, is a promising natural product with both nutritional and pharmaceutical applications. In this study, light-related strategies were applied to enhance the cell growth and lutein production of a lutein-rich microalga Scenedesmus obliquus FSP-3. The results demonstrate that using white LED resulted in better lutein production efficiency when compared to the other three monochromatic LEDs (red, blue, and green). The lutein productivity of S. obliquus FSP-3 was further improved by adjusting the type of light source and light intensity. The optimal lutein productivity of 4.08 mg/L/d was obtained when using a TL5 fluorescent lamp at a light intensity of 300 μmol/m(2)/s, and this performance is better than that reported in most related studies. Moreover, the time-course profile of lutein accumulation in the microalga shows that the maximal lutein content and productivity were obtained at the onset of nitrogen depletion.

Published
2014

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