1. Key extracellular enzymes triggered high-efficiency composting associated with bacterial community succession
- Author
-
Yannan Ou, Xu Xu, Rong Li, Chao Liu, Zhengyang Liu, Zongzhuan Shen, Qiao Cece, Qirong Shen, and C. Ryan Penton
- Subjects
0106 biological sciences ,Environmental Engineering ,Achromobacter ,Bioengineering ,Cellulase ,010501 environmental sciences ,complex mixtures ,01 natural sciences ,chemistry.chemical_compound ,Soil ,Clostridium ,010608 biotechnology ,Virgibacillus ,Hemicellulose ,Food science ,Cellulose ,Waste Management and Disposal ,0105 earth and related environmental sciences ,biology ,Bacteria ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Thermophile ,Composting ,beta-Glucosidase ,fungi ,General Medicine ,biology.organism_classification ,biology.protein ,Mesophile - Abstract
A consortium of key bacterial taxa plays critical roles in the composting process. In order to elucidate the identity and mechanisms by which specific bacterial species drive high-efficiency composting, the succession of key bacterial consortia and extracellular enzymes produced during the composting process were monitored in composting piles with varying initial C/N ratios. Results showed that C/N ratios of 25 and 35 enhanced composting efficiency through elevated temperatures, higher germination indices, enhanced cellulose and hemicellulose degradation, and higher cellulase and dehydrogenase activities. The activities of cellulase and β-glucosidase, cellulase and protease, and cellulase and β-glucosidase exhibited significant relationships with bacterial community composition within the mesophilic, thermophilic, and mature phases, respectively. Putative key taxa, linked to a higher composting efficiency, such as Nonomuraea, Desemzia, Cellulosimicrobium, Virgibacillus, Clostridium, and Achromobacter, exhibited significantly positive relationships with extracellular enzyme activities, suggesting a significant contribution to these taxa to the development of composting maturity.
- Published
- 2019