1. Temperature dependent (37-15°C) anaerobic digestion of a trichloroethylene-contaminated wastewater
- Author
-
Anne-Marie Enright, Vincent O'Flaherty, and Alma Siggins
- Subjects
Environmental Engineering ,Trichloroethylene ,Biomass ,Bioengineering ,Biology ,Polymerase Chain Reaction ,chemistry.chemical_compound ,Bioreactors ,RNA, Ribosomal, 16S ,Bioreactor ,Anaerobiosis ,Waste Management and Disposal ,Phylogeny ,DNA Primers ,Base Sequence ,Renewable Energy, Sustainability and the Environment ,Environmental engineering ,Temperature ,General Medicine ,Contamination ,Archaea ,Anaerobic digestion ,Microbial population biology ,Wastewater ,chemistry ,Environmental chemistry ,Electrophoresis, Polyacrylamide Gel ,Temperature gradient gel electrophoresis ,Water Pollutants, Chemical - Abstract
The impact of a trichloroethylene (TCE) contaminated wastewater on the microbial community structure of an anaerobic granular biomass at 15 °C compared to 37 °C was investigated. Four expanded granular sludge bed (EGSB) bioreactors (R1–R4) were employed in pairs at 37 and 15 °C. The influents of one of each pair were supplemented with increasing concentrations of TCE (max. 60 mg l−1). At 37 °C, stable operation was maintained with 88% COD removal and >99% TCE removal at maximum influent TCE concentrations. R3 performance decreased at influent TCE concentration of 60 mg l−1, although TCE removal rates of >97% were recorded. Archaeal community analysis via clone library and quantitative polymerase chain reaction (qPCR) analysis, and bacterial community analysis via denaturing gradient gel electrophoresis (DGGE), indicated that temperature resulted in a greater change in community structure than the presence of TCE, and clones related to cold adaptation of biomass were identified at 15 °C.
- Published
- 2011