Search

[Display omitted] • A potential lutein-producing isolate identified as Chlorella sorokiniana Kh12. • A 2X-HT-9 k condition offered the maximum lutein yield (47.36 mg l−1) under mixotrophy. • It has yielded maximum lutein 13.69 mg g−1, one of the best yields reported so far. • Bead milling was most effective to disrupt algal cell wall and lutein extraction. • Seven minutes bead milling was most efficient for maximum lutein extraction. A bioprospecting study was conducted from Seawater samples collected at Kaohsiung Seacoast, Taiwan. The current research was aimed to isolate potential lutein-producing strain, evaluate and optimize the best cultivation mode, lutein accumulation stage, lutein-extraction method, and condition to recover maximum lutein (main product) and lipid (byproduct). Biorefinery is the latest approach worldwide to extract multi-products for cost-effectiveness. Selected isolate among several isolates, identified as Chlorella sorokiniana Kh12 and exploited under biorefinery concept for lutein and lipid extraction. Kh12 cultivated under mixotrophy: 2X-(HT)-9k yielded maximum biomass (3.46 g L−1) and lutein (13.69 mg g−1) which is among the higher yields reported so far. Among various tested solvents, methanol was the best extractor. Bead milling was most effective to disrupt algal cell walls, seven minutes of milling was best for maximum lutein (7.56 mg g−1) extraction. Kh12 could be a promising candidate for commercial lutein and lipid co-production based on the outcome. [ABSTRACT FROM AUTHOR]