1. Deciphering styrene oxide tolerance mechanisms in Gluconobacter oxydans mutant strain.
- Author
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Chen, Yan, Liu, Fei, Sha, Aobo, Xu, Meijuan, Rao, Zhiming, and Zhang, Xian
- Subjects
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STYRENE oxide , *POLLUTANTS , *BIOLOGICAL evolution , *GENETIC engineering , *ORGANIC solvents - Abstract
[Display omitted] • A broad-spectrum organic solvents tolerant strain STO was obtained by ALE. • Multi-omics integrated physiology of styrene oxide tolerant strain was analyzed. • GOX_RS04415 and GOX_RS04700 were considered as key genes for tolerance in G. oxydans. • The first report on the organic solvent tolerance mechanisms in G. oxydans. Chemical production wastewater contains large amounts of organic solvents (OSs), which pose a significant threat to the environment. In this study, a 10 g·L−1 styrene oxide tolerant strain with broad-spectrum OSs tolerance was obtained via adaptive laboratory evolution. The mechanisms underlying the high OS tolerance of tolerant strain were investigated by integrating physiological, multi-omics, and genetic engineering analyses. Physiological changes are one of the main factors responsible for the high OS tolerance in mutant strains. Moreover, the P-type ATPase GOX_RS04415 and the LysR family transcriptional regulator GOX_RS04700 were also verified as critical genes for styrene oxide tolerance. The tolerance mechanisms of OSs can be used in biocatalytic chassis cell factories to synthesize compounds and degrade environmental pollutants. This study provides new insights into the mechanisms underlying the toxicological response to OS stress and offers potential targets for enhancing the solvent tolerance of G. oxydans. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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