1. Phospho-mTOR (Ser2481) colocalizes with condensed chromosomes during metaphase.
- Author
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Kosach, V. R., Tykhonkova, I. O., Cherednyk, O. V., Filonenko, V. V., and Khoruzhenko, A. I.
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CHROMOSOMES , *METAPHASE (Mitosis) , *RAPAMYCIN - Abstract
Aim. To study the subcellular localization of phospho-mTOR (Ser2481) in cultured cells of human cancer cell lines. Methods. Immunofluorescent analysis of phospho-mTOR (Ser2481) subcellular localization in cultured MCF-7 (human breast adenocarcinoma) and HepG2 (human liver carcinoma) cells. Results. For the first time phospho-mTOR (Ser2481) was detected as bright spots situated in the equatorial region of chromosomes and co-localized with condensed chromosomes during metaphase. Conclusions. In this study, we describe for the first time the co-localization of phospho-mTOR (Ser2481) and chromosomes of the metaphase plate in the MCF-7 and HepG2 cells. Our data support the hypothesis that phospho-mTOR (Ser2481) could act as a CPP (chromosomal passenger protein)-like kinase, which takes part in the regulation of mitosis progression. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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