Search

Your search keyword '"Kozlov, Michael M"' showing total 33 results
Start Over Author "Kozlov, Michael M" Journal biophysical journal
33 results on '"Kozlov, Michael M"'

15. Correction

Author

Elia, Natalie, primary, Fabrikant, Gur, additional, Kozlov, Michael M., additional, and Lippincott-Schwartz, Jennifer, additional

Published
2012
Full Text
View/download PDF

33. Role of focal adhesions and mechanical stresses in the formation and progression of the lamellipodium-lamellum interface [corrected].

Author

Shemesh T, Verkhovsky AB, Svitkina TM, Bershadsky AD, and Kozlov MM

Subjects
Animals, Cell Line, Computer Simulation, Cornea cytology, Cornea physiology, Elasticity, Fibroblasts cytology, Fibroblasts physiology, Fibroblasts ultrastructure, Fishes, Friction physiology, Mice, Rats, Stromal Cells cytology, Stromal Cells physiology, Swiss 3T3 Cells, Video Recording, Xenopus, Actins metabolism, Cell Movement physiology, Focal Adhesions physiology, Models, Biological, Pseudopodia physiology, Stress, Mechanical
Abstract

Actin network in the front part of a moving cell is organized into a lamellipodium and a lamellum. A distinct lamellipodium-lamellum interface is associated with focal adhesions and consists of a series of arclike segments linking neighboring focal adhesions in the front row. The interface advances by leaping onto new rows of focal adhesions maturating underneath the lamellipodium. We propose a mechanism of the lamellipodium-lamellum boundary generation, shape formation, and progression based on the elastic stresses generated in the lamellipodial actin gel by its friction against the focal adhesions. The crucial assumption of the model is that stretching stresses trigger actin gel disintegration. We compute the stress distribution throughout the actin gel and show that the gel-disintegrating stresses drive formation of a gel boundary passing through the row of focal adhesions. Our computations recover the lamellipodium-lamellum boundary shapes detected in cells and predict the mode of the boundary transition to the row of the newly maturing focal adhesions in agreement with the experimental observations. The model fully accounts for the current phenomenology of the lamellipodium-lamellum interface formation and advancing, and makes experimentally testable predictions on the dependence of these phenomena on the sizes of the focal adhesions, the character of the focal adhesion distribution on the substrate, and the velocity of the actin retrograde flow with respect to the focal adhesions. The phase diagram resulting from the model provides a background for quantitative classification of different cell types with respect to their ability to form a lamellipodium-lamellum interface. In addition, the model suggests a mechanism of nucleation of the dorsal and arclike actin bundles found in the lamellum.

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results