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Your search keyword '"Holopainen, Juha M."' showing total 15 results
Start Over Author "Holopainen, Juha M." Journal biophysical journal
15 results on '"Holopainen, Juha M."'

1. Molecular Organization of the Tear Fluid Lipid Layer

Author

Kulovesi, Pipsa, primary, Telenius, Jelena, additional, Koivuniemi, Artturi, additional, Brezesinski, Gerald, additional, Rantamäki, Antti, additional, Viitala, Tapani, additional, Puukilainen, Esa, additional, Ritala, Mikko, additional, Wiedmer, Susanne K., additional, Vattulainen, Ilpo, additional, and Holopainen, Juha M., additional

Published
2010
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15. Macroscopic consequences of the action of phospholipase C on giant unilamellar liposomes.

Author

Holopainen JM, Angelova MI, Söderlund T, and Kinnunen PK

Subjects
Bacillus cereus enzymology, Biophysical Phenomena, Biophysics, Chromatography, Thin Layer, Membranes, Artificial, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Platinum chemistry, Temperature, Time Factors, Liposomes metabolism, Type C Phospholipases chemistry, Type C Phospholipases metabolism
Abstract

Macroscopic consequences of the formation of diacylglycerol by phospholipase C (PC-PLC) in giant 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC) unilamellar vesicles (GUVs, diameter 10-100 microm) were studied by phase contrast and fluorescence microscopy. PC-PLC caused a series of fast stepwise shrinkages of fluid SOPC GUVs, continuing until the vesicle disappeared beyond the optical resolution of the microscope. The presence of N-palmitoyl-sphingomyelin (mole fraction X = 0.25) in the GUVs did not affect the outcome of the PC-PLC reaction. In addition to hydrolysis, PC-PLC induced adhesion of vicinal vesicles. When multilamellar SOPC vesicles were used only a minor decrease in their diameter was evident suggesting that PC-PLC can exert its hydrolytic activity only in the outer monolayer. A series of stepwise shrinkages was observed also for 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) GUVs above their main phase transition temperature, T(m), i.e., when the bilayer is in the liquid crystalline state. However, this process was not observed for DMPC GUVs in the gel state, below T(m). These results are supported by the enhanced activity of PC-PLC upon exceeding T(m) of DMPC large unilamellar vesicles (diameter approximately 0.1 microm) used as a substrate. Studies on SOPC monolayers revealed that PC-PLC can exert its hydrolytic activity only at surface pressures below approximately 30 mN/m. Accordingly, the lack of changes in the gel state DMPC GUVs could be explained by the equilibrium lateral pressure in these vesicles exceeding this critical value.

Published
2002
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