1. 13C-NMR of ribosyl A-A-A, A-A-G, AND A-U-G Synthesis and Assignment
- Author
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Stephen A. Winkle, Philip N. Borer, Gloria D. McFarland, Michael P. Stone, and Min C. Yoo
- Subjects
Magnetic Resonance Spectroscopy ,Oligoribonucleotides ,HPLC - High pressure liquid chromatography ,Base Sequence ,Adenine Nucleotides ,Chemistry ,Stereochemistry ,Organic Chemistry ,Biophysics ,RNA ,Nuclear magnetic resonance spectroscopy ,Carbon-13 NMR ,Biochemistry ,Chemical synthesis ,Structure-Activity Relationship ,Nucleic Acid Conformation - Abstract
The three RNA trinucleotides; ApApA, ApApG, and ApUpG, have been synthesized in sufficient quantity to obtain natural abundance 13 C{ 1 H}-NMR spectra at strand concentrations between 4 and 100 mM. Comparisons between 70°C spectra of the three trimers and their consistuent dimers ApA, ApG, ApU, and UpG allow secure assignments to be made for most of the resonances. This paper describes the syntheses and 13 C assignments of the oligomers.
- Published
- 1985
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