1. Detection of apoptosis by PET/CT with the diethyl ester of [¹⁸F]ML-10 and fluorescence imaging with a dansyl analogue
- Author
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Manikandan, Kadirvel, Michael, Fairclough, Christopher, Cawthorne, Emily J, Rowling, Muhammad, Babur, Adam, McMahon, Paul, Birkket, Alison, Smigova, Sally, Freeman, Kaye J, Williams, and Gavin, Brown
- Subjects
Male ,Fluorine Radioisotopes ,Mice ,Radiochemistry ,Halogenation ,Positron-Emission Tomography ,Animals ,Humans ,Apoptosis ,Esters ,Radiopharmaceuticals ,Tomography, X-Ray Computed - Abstract
The diethyl ester of [(18)F]ML-10 is a small molecule apoptotic PET probe for cancer studies. Here we report a novel multi-step synthesis of the diethyl ester of ML-10 in excellent yields via fluorination using Xtal-Fluor-E. In addition, a one-pot radiosynthesis of the diethyl ester of [(18)F]ML-10 from nucleophilic [(18)F]fluoride was completed in 23% radiochemical yield (decay corrected). The radiochemical purity of the product was ≥99%. The diethyl ester of [(18)F]ML-10 was used in vivo to detect apoptosis in the testes of mice. In parallel studies, the dansyl-ML-10 diethyl ester was prepared and used to detect apoptotic cells in an in vitro cell based assay.
- Published
- 2013