1. [Changes in the antigenic properties of proteins of laboratory mice during oxidative stress].
- Author
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Rasskazova EA and Sadovnikov VB
- Subjects
- Aging blood, Aging immunology, Aging metabolism, Aging radiation effects, Animals, Brain metabolism, Brain radiation effects, Gamma Rays adverse effects, Liver metabolism, Liver radiation effects, Male, Mice, Mice, Inbred Strains, Myocardium metabolism, Nuclear Proteins chemistry, Nuclear Proteins immunology, Nuclear Proteins radiation effects, Oxidative Stress radiation effects, Protein Carbonylation immunology, Protein Carbonylation radiation effects, Proteins radiation effects, Spleen metabolism, Spleen radiation effects, Subcellular Fractions metabolism, Subcellular Fractions radiation effects, Antigens blood, Oxidative Stress immunology, Proteins chemistry, Proteins immunology
- Abstract
Changes in the level of oxidative damage to proteins in CD1 outbred mice gamma irradiated with a dose of 3 Gy have been studied. The changes were estimated from the amount of carbonyl groups (CG) in the proteins. It was found that two hours after exposure to gamma radiation, the amount of CG in the cytoplasmic and nuclear fractions of the liver, heart, brain, and spleen sharply increased. Two months after irradiation, the level of CG in the cytoplasmic and nuclear subcellular fractions of the liver and brain decreased to the level of CG in the control animals, which were not exposed to radiation. In the subcellular fractions of the heart and spleen, the increase in the degree of damage was more significant and a high level of damage was observed even two months after irradiation. An enhancement of the antigenic properties of proteins from the liver, heart, and spleen in the postirradiation period was found. Spleen proteins were most immunogenic. A comparison of the antigenic properties of proteins isolated from the tissues 60 days after irradiation revealed a correlation between the level of oxidative damage and the immunogenicity of the total protein fraction.
- Published
- 2009
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