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Start Over Author "O. P. Kovalenko" Journal bioorganicheskaia khimiia
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1. [Comparative study of the reactability of phosphoric acid residues in tRNA(Ser) and tRNA(Leu) from Thermus thermophilus]

Author

O P, Kovalenko, Z M, Petrushenko, I A, Kriklivyĭ, A D, Iaremchuk, and M A, Tukalo

Subjects
RNA, Transfer, Leu, Base Sequence, Thermus thermophilus, Molecular Sequence Data, Anticodon, Nucleic Acid Conformation, Electrophoresis, Polyacrylamide Gel, Phosphoric Acids, RNA, Transfer, Ser
Abstract

The reactivity of phosphates in the Thermus thermophilus tRNA(Ser) (GCU) and tRNA(Leu) (CAG) was studied using the ethylnitrosourea modification. It was shown that phosphates of nucleotides 58-60 (T loop), 20-22 (D loop), and 48 (at the junction of the variable and T stems) were poorly modified in both tRNAs. The most pronounced differences in the reactivity were observed for phosphates at the junctions of the variable stem with T-stem (47q, 49) and anticodon stem (45). This indicates differences in orientations of the long variable arm relative to the backbone in the tRNAs studied.

Published
2000

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