1. Synthesis and transfection activity of novel galactosylated polycationic lipid.
- Author
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Maslov MA, Medvedeva DA, Rapoport DA, Serikov RN, Morozova NG, Serebrennikova GA, Vlassov VV, and Zenkova MA
- Subjects
- Animals, Cell Line, Cell Survival drug effects, Cricetinae, Flow Cytometry, Gene Transfer Techniques, Humans, Lipids chemistry, Lipids toxicity, Liposomes chemistry, Microscopy, Atomic Force, Molecular Structure, Polyelectrolytes, Transfection, Galactose chemistry, Lipids chemical synthesis, Polyamines chemistry
- Abstract
In this study, we synthesized a new galactosylated cationic lipid and investigated its biological activity. The structure of lipid combines both spermine residue for DNA compaction and galactose moiety for the improvement of aggregation behavior of lipoplexes. Lipid was low toxic for different mammalian cells, and was able both to compact plasmid DNA and to mediate cellular accumulation of various nucleic acids (ODN, pDNA and siRNA) exhibiting biological activity (transgene expression, gene silencing)., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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