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Start Over Descriptor "Thiazoles standards" Journal bioorganic medicinal chemistry

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1. Thiazole orange as the fluorescent intercalator in a high resolution fid assay for determining DNA binding affinity and sequence selectivity of small molecules.

Author

Boger DL and Tse WC

Subjects
Anti-Bacterial Agents metabolism, Benzothiazoles, Binding, Competitive, Ethidium chemistry, Ethidium standards, Fluorescent Dyes chemistry, Gene Library, Intercalating Agents standards, Ligands, Microchemistry, Netropsin metabolism, Oligodeoxyribonucleotides metabolism, Quinolines, Spectrometry, Fluorescence, Thiazoles standards, Titrimetry, DNA metabolism, Fluorescent Dyes standards, Intercalating Agents chemistry, Thiazoles chemistry
Abstract

The viability of using thiazole orange as an alternative to ethidium bromide in a fluorescent intercalator displacement (FID) assay is explored by profiling the DNA binding affinity and sequence selectivity of netropsin. Utilizing a library of hairpin deoxyoligonucleotides containing all possible four base-pair sequences, the method provides a high resolution profile of the DNA binding properties of small molecules in a high throughput format.

Published
2001
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