1. Yarrowia lipolytica dehydrogenase/reductase: An enzyme tolerant for lipophilic compounds and carbohydrate substrates.
- Author
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Napora, Kamila, Wrodnigg, Tanja M., Kosmus, Patrick, Thonhofer, Martin, Robins, Karen, and Winkler, Margit
- Subjects
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DIPODASCACEAE , *DEHYDROGENASES , *CARBOHYDRATES , *REDUCTASES , *GENE expression , *OXIDATION , *MANNITOL - Abstract
Abstract: Yarrowia lipolytica short chain dehydrogenase/reductase (YlSDR) was expressed in Escherichia coli, purified and characterized in vitro. The substrate scope for YlSDR mediated oxidation was investigated with alcohols and unprotected carbohydrates spectrophotometrically, revealing a preference for secondary compared to primary alcohols. In reduction direction, YlSDR was highly active on ribulose and fructose, suggesting that the enzyme is a mannitol-2-dehydrogenase. In order to explore substrate tolerance especially for space-demanding, lipophilic protecting groups, 5-O-trityl-d-ribitol and 5-O-trityl-α,β-d-ribose were investigated as substrates: YlSDR oxidized 5-O-trityl-d-ribitol and 5-O-trityl-α,β-d-ribose and reduced the latter at the expense of NADP(H). [Copyright &y& Elsevier]
- Published
- 2013
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