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Your search keyword '"Interferon-Induced Helicase"' showing total 2 results
Start Over Descriptor "Interferon-Induced Helicase" Journal biomolecules
2 results on '"Interferon-Induced Helicase"'

1. Vitamin C Enhances Antiviral Functions of Lung Epithelial Cells.

Author

Teafatiller, Trevor, Agrawal, Sudhanshu, De Robles, Gabriela, Rahmatpanah, Farah, Subramanian, Veedamali S, and Agrawal, Anshu

Subjects
Bronchi, Cell Line, Transformed, Epithelial Cells, Animals, Mice, Knockout, Humans, Mice, Ascorbic Acid, Interferon-alpha, Receptors, Immunologic, Receptors, Retinoic Acid, Poly I-C, Interleukin-8, Interleukin-6, Antiviral Agents, Gene Expression Regulation, Biological Transport, L-Gulonolactone Oxidase, Interferon Regulatory Factors, Sodium-Coupled Vitamin C Transporters, Transcriptome, Myxovirus Resistance Proteins, Interferon-Induced Helicase, IFIH1, DEAD Box Protein 58, GULO-KO mice, ISGs, airway epithelial cells, antiviral responses, vitamin C, vitamin C transporters, Lung, Nutrition, Genetics, Prevention, Complementary and Integrative Health, 2.1 Biological and endogenous factors, Underpinning research, Aetiology, 1.1 Normal biological development and functioning, Inflammatory and immune system, Respiratory, Biochemistry and Cell Biology
Abstract

Vitamin C is well documented to have antiviral functions; however, there is limited information about its effect on airway epithelial cells-the first cells to encounter infections. Here, we examined the effect of vitamin C on human bronchial epithelium transformed with Ad12-SV40 2B (BEAS-2B) cells, and observed that sodium-dependent vitamin C transporter 2 (SVCT2) was the primary vitamin C transporter. Transcriptomic analysis revealed that treating BEAS-2B cells with vitamin C led to a significant upregulation of several metabolic pathways and interferon-stimulated genes (ISGs) along with a downregulation of pathways involved in lung injury and inflammation. Remarkably, vitamin C also enhanced the expression of the viral-sensing receptors retinoic acid-inducible gene 1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA-5), which was confirmed at the protein and functional levels. In addition, the lungs of l-gulono-γ-lactone oxidase knockout (GULO-KO) mice also displayed a marked decrease in these genes compared to wild-type controls. Collectively, our findings indicate that vitamin C acts at multiple levels to exert its antiviral and protective functions in the lungs.

Published
2021

2. Vitamin C Enhances Antiviral Functions of Lung Epithelial Cells

Author

Anshu Agrawal, Farah Rahmatpanah, Gabriela De Robles, Veedamali S. Subramanian, Sudhanshu Agrawal, and Trevor Teafatiller

Subjects
Myxovirus Resistance Proteins, 0301 basic medicine, Interferon-Induced Helicase, IFIH1, Receptors, Retinoic Acid, Retinoic Acid, vitamin C, Ascorbic Acid, ISGs, Biochemistry, Transcriptome, Mice, 0302 clinical medicine, Immunologic, Receptors, 2.1 Biological and endogenous factors, GULO-KO mice, Receptors, Immunologic, Aetiology, Interferon-Induced Helicase, Receptor, Lung, Cell Line, Transformed, IFIH1, Mice, Knockout, Oxidase test, Chemistry, QR1-502, antiviral responses, 030220 oncology & carcinogenesis, Interferon Regulatory Factors, Respiratory, DEAD Box Protein 58, medicine.symptom, L-Gulonolactone Oxidase, Knockout, 1.1 Normal biological development and functioning, Bronchi, Inflammation, Lung injury, Antiviral Agents, Microbiology, Article, Cell Line, 03 medical and health sciences, Downregulation and upregulation, Underpinning research, vitamin C transporters, Complementary and Integrative Health, Genetics, medicine, Animals, Humans, Sodium-Coupled Vitamin C Transporters, Molecular Biology, Nutrition, Vitamin C, Interleukin-6, Prevention, Inflammatory and immune system, Interleukin-8, Interferon-alpha, Epithelial Cells, Biological Transport, Transporter, Molecular biology, stomatognathic diseases, Poly I-C, 030104 developmental biology, Transformed, Gene Expression Regulation, Biochemistry and Cell Biology, airway epithelial cells
Abstract

Vitamin C is well documented to have antiviral functions, however, there is limited information about its effect on airway epithelial cells—the first cells to encounter infections. Here, we examined the effect of vitamin C on human bronchial epithelium transformed with Ad12-SV40 2B (BEAS-2B) cells, and observed that sodium-dependent vitamin C transporter 2 (SVCT2) was the primary vitamin C transporter. Transcriptomic analysis revealed that treating BEAS-2B cells with vitamin C led to a significant upregulation of several metabolic pathways and interferon-stimulated genes (ISGs) along with a downregulation of pathways involved in lung injury and inflammation. Remarkably, vitamin C also enhanced the expression of the viral-sensing receptors retinoic acid-inducible gene 1 (RIG-1) and melanoma differentiation-associated protein 5 (MDA-5), which was confirmed at the protein and functional levels. In addition, the lungs of l-gulono-γ-lactone oxidase knockout (GULO-KO) mice also displayed a marked decrease in these genes compared to wild-type controls. Collectively, our findings indicate that vitamin C acts at multiple levels to exert its antiviral and protective functions in the lungs.

Published
2021

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