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Your search keyword '"Exteins"' showing total 2 results
Start Over Descriptor "Exteins" Journal biomolecular nmr assignments
2 results on '"Exteins"'

1. Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine

Author

Marlene Belfort, C. Seth Pearson, Alexander Shekhtman, and Georges Belfort

Subjects
Stereochemistry, Proton Magnetic Resonance Spectroscopy, Biology, Protein Engineering, Biochemistry, Article, Inteins, Catalysis, Rec A Recombinases, Protein structure, Structural Biology, Protein splicing, Exteins, RNA splicing, Biocatalysis, Triple-resonance nuclear magnetic resonance spectroscopy, Cysteine, Intein, Nuclear Magnetic Resonance, Biomolecular
Abstract

The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.

Published
2014

2. Backbone assignments of mini-RecA intein with short native exteins and an active N-terminal catalytic cysteine.

Author

Pearson CS, Belfort G, Belfort M, and Shekhtman A

Subjects
Biocatalysis, Protein Engineering, Proton Magnetic Resonance Spectroscopy, Cysteine chemistry, Exteins, Inteins, Nuclear Magnetic Resonance, Biomolecular, Rec A Recombinases chemistry
Abstract

The backbone resonance assignments of an engineered splicing-inactive mini-RecA intein based on triple resonance experiments with [(13)C,(15)N]-labeled protein are reported. The construct contains inactivating mutations specifically designed to retain most catalytic residues, especially those that are potentially metal-coordinating. The assignments are essential for protein structure determination of a precursor with an active N-terminal catalytic cysteine and for investigation of the atomic details of splicing.

Published
2015
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