1. Equisetum arvense standardized dried extract hinders age-related osteosarcopenia.
- Author
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Salvadori L, Paiella M, Castiglioni B, Belladonna ML, Manenti T, Ercolani C, Cornioli L, Clemente N, Scircoli A, Sardella R, Tensi L, Astolfi A, Barreca ML, Chiappalupi S, Gentili G, Bosetti M, Sorci G, Filigheddu N, and Riuzzi F
- Subjects
- Animals, Mice, RAW 264.7 Cells, Muscle Fibers, Skeletal drug effects, Muscle Fibers, Skeletal pathology, Muscle Fibers, Skeletal metabolism, Aging drug effects, Aging pathology, Muscular Atrophy drug therapy, Muscular Atrophy pathology, Osteoclasts drug effects, Osteoclasts metabolism, Osteoclasts pathology, RANK Ligand metabolism, NF-kappa B metabolism, Osteogenesis drug effects, Anti-Inflammatory Agents pharmacology, Plant Extracts pharmacology, Plant Extracts therapeutic use, Sarcopenia drug therapy, Sarcopenia pathology, Equisetum chemistry
- Abstract
Age-associated osteosarcopenia is an unresolved syndrome characterized by the concomitant loss of bone (osteopenia) and skeletal muscle (sarcopenia) tissues increasing falls, immobility, morbidity, and mortality. Unbalanced resorption of bone in the remodeling process and excessive protein breakdown, especially fast type II myosin heavy chain (MyHC-II) isoform and myofiber metabolic shift, are the leading causes of bone and muscle deterioration in the elderly, respectively. Equisetum arvense (EQ) is a plant traditionally recommended for many pathological conditions due to its anti-inflammatory properties. Thus, considering that a chronic low-grade inflammatory state predisposes to both osteoporosis and sarcopenia, we tested a standardized hydroalcoholic extract of EQ in in vitro models of muscle atrophy [C2C12 myotubes treated with proinflammatory cytokines (TNFα/IFNγ), excess glucocorticoids (dexamethasone), or the osteokine, receptor activator of nuclear factor kappa-B ligand (RANKL)] and osteoclastogenesis (RAW 264.7 cells treated with RANKL). We found that EQ counteracted myotube atrophy, blunting the activity of several pathways depending on the applied stimulus, and reduced osteoclast formation and activity. By in silico target fishing, IKKB-dependent nuclear factor kappa-B (NF-κB) inhibition emerges as a potential common mechanism underlying EQ's anti-atrophic effects. Consumption of EQ (500 mg/kg/day) by pre-geriatric C57BL/6 mice for 3 months translated into: i) maintenance of muscle mass and performance; ii) restrained myofiber oxidative shift; iii) slowed down age-related modifications in osteoporotic bone, significantly preserving trabecular connectivity density; iv) reduced muscle- and spleen-related inflammation. EQ can preserve muscle functionality and bone remodeling during aging, potentially valuable as a natural treatment for osteosarcopenia., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.)
- Published
- 2024
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